![Western Blot: NEK11 Antibody (4E1-1F4) [H00079858-M01] - NEK11 monoclonal antibody (M01), clone 4E1-1F4. Analysis of NEK11 expression in A-431.](http://images.novusbio.com/fullsize/NEK11-Antibody-4E1-1F4-Western-Blot-H00079858-M01-img0012.jpg "Western Blot: NEK11 Antibody (4E1-1F4) [H00079858-M01] - NEK11 monoclonal antibody (M01), clone 4E1-1F4. Analysis of NEK11 expression in A-431.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clone | 4E1-1F4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | NEK11 (AAH28587, 1 a.a. ~ 482 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MLKFQEAAKCVSGSTAISTYPKTLIARRYVLQQKLGSGSFGTVYLVSDKKAKRGEELKVLKEISVGELNPNETVQANLEAQLLSKLDHPAIVKFHASFVEQDNFCIITEYCEGRDLDDKIQEYKQAGKIFPENQIIEWFIQLLLGVDYMHERRILHRDLKSKNVFLKNNLLKIGDFGVSRLLMGSCDLATTLTGTPHYMSPEALKHQGYDTKSDIWSLACILYEMCCMNHAFAGSNFLSIVLKIVEGDTPSLPERYPKELNAIMESMLNKNPSLRPSAIEILKIPYLDEQLQNLMCRYSEMTLEDKNLDCQKEAAHIINAMQKRIHLQTLRALSEVQKMTPRERMRLRKLQAADEKARKLKKIVEEKYEENSKRMQELRSRNFQQLSVDVLHEKTHLKGMEEKEEQPEGRLSCSPQDEDEERWQGREEESDEPTLENLPESQPIPSMDLHELESIVEDATSDLGYHGDCNLISLDEYWKNEK |
| Specificity | NEK11 - NIMA (never in mitosis gene a)- related kinase 11 |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NEK11 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: NEK11 Antibody (4E1-1F4) [H00079858-M01] - Analysis of monoclonal antibody to NEK11 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/NEK11-Antibody-4E1-1F4-Immunocytochemistry-Immunofluorescence-H00079858-M01-img0007.jpg "Immunocytochemistry/Immunofluorescence: NEK11 Antibody (4E1-1F4) [H00079858-M01] - Analysis of monoclonal antibody to NEK11 on HeLa cell. Antibody concentration 10 ug/ml.")
![Immunohistochemistry-Paraffin: NEK11 Antibody (4E1-1F4) [H00079858-M01] - Analysis of monoclonal antibody to NEK11 on formalin-fixed paraffin-embedded human endometrium tissue. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/NEK11-Antibody-4E1-1F4-Immunohistochemistry-Paraffin-H00079858-M01-img0009.jpg "Immunohistochemistry-Paraffin: NEK11 Antibody (4E1-1F4) [H00079858-M01] - Analysis of monoclonal antibody to NEK11 on formalin-fixed paraffin-embedded human endometrium tissue. Antibody concentration 3 ug/ml.")
![Western Blot: NEK11 Antibody (4E1-1F4) [H00079858-M01] - NEK11 monoclonal antibody (M01), clone 4E1-1F4 Analysis of NEK11 expression in HL-60.](http://images.novusbio.com/fullsize/NEK11-Antibody-4E1-1F4-Western-Blot-H00079858-M01-img0011.jpg "Western Blot: NEK11 Antibody (4E1-1F4) [H00079858-M01] - NEK11 monoclonal antibody (M01), clone 4E1-1F4 Analysis of NEK11 expression in HL-60.")
![Western Blot: NEK11 Antibody (4E1-1F4) [H00079858-M01] - Analysis of NEK11 expression in transfected 293T cell line by NEK11 monoclonal antibody (M01), clone 4E1-1F4.Lane 1: NEK11 transfected lysate(55.5 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NEK11-Antibody-4E1-1F4-Western-Blot-H00079858-M01-img0013.jpg "Western Blot: NEK11 Antibody (4E1-1F4) [H00079858-M01] - Analysis of NEK11 expression in transfected 293T cell line by NEK11 monoclonal antibody (M01), clone 4E1-1F4.Lane 1: NEK11 transfected lysate(55.5 KDa).Lane 2: Non-transfected lysate.")
![Sandwich ELISA: NEK11 Antibody (4E1-1F4) [H00079858-M01] - Detection limit for recombinant GST tagged NEK11 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NEK11-Antibody-4E1-1F4-Sandwich-ELISA-H00079858-M01-img0010.jpg "Sandwich ELISA: NEK11 Antibody (4E1-1F4) [H00079858-M01] - Detection limit for recombinant GST tagged NEK11 is 0.1 ng/ml as a capture antibody.")
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")