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NDUFA7 Antibody

Images

 
Western Blot: NDUFA7 Antibody [NBP2-26161] - Human (A), Mouse (B) and Rat (C) Heart lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: NDUFA7 Antibody [NBP2-26161] - Analysis of Human Heart shows mitochondrial staining in cardial fibres. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: NDUFA7 Antibody [NBP2-26161] - Pig Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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NDUFA7 Antibody Summary

Immunogen
Peptide with sequence C-PKLPVGPSHKLSNN corresponding to internal region according to NP_004992.2.
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NDUFA7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 4 - 8 ug/ml
  • Immunohistochemistry-Paraffin 4 - 8 ug/ml
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.03 - 0.1 ug/ml
Application Notes
WB: Approx. 15 kDa band observed in human, mouse, rat and porcine heart lysates (calculated MW of 12.6 kDa band according to NP_004992.2). IHC-P: Human heart shows mitochondrial staining in cardial fibres.
Publications
Read Publication using NBP2-26161.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NDUFA7 Antibody

  • B14.5a
  • CI-B14.5a
  • complex I B14.5a subunit
  • Complex I-B14.5a
  • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 7 (14.5kD, B14.5a)
  • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 7, 14.5kDa
  • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7
  • NADH-ubiquinone oxidoreductase subunit B14.5a

Background

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NDUFA7 Antibody (NBP2-26161)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NDUFA7
Entrez