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Myosin 1B Antibody

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Western Blot: Myosin 1B Antibody [NBP1-97778] - (1ug/ml) Analysis of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Myosin 1B Antibody Summary

Immunogen
Peptide with sequence C-RTVEAKQEKVSTT corresponding to internal region according to NP_036355.2.
Specificity
This antibody is expected to recognize both reported isoforms (NP_001123630.1; NP_036355.2). Reported variants represent identical protein: NP_001123630.1, NP_001155291.1.
Predicted Species
Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MYO1B
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5 - 10 ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
Application Notes
WB: Approx. 120 kDa band observed in NIH3T3 lysates (calculated MW of 125 kDa band according to human NP_036355.2 and 128 kDa band according to mouse NP_034993.2). IHC-P: Human cerebral cortex shows cytoplasm staining in the neuronal bodies.
Publications
Read Publication using NBP1-97778.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Myosin 1B Antibody

  • MMIa
  • MMI-alpha
  • MYH-1c
  • MYO1B variant protein
  • Myosin I alpha
  • myosin IB
  • myosin-I alpha
  • myosin-Ib
  • myr1

Background

Myosin 1B is a motor protein which is involved in neuronal development, membrane trafficking/dynamics and functions including cell migration, vesicular transport and neurite outgrowth. Myosin 1B is known to have interactions with other proteins such as MAP3K3, RIPK3, BRF2, EIF4A2 and CIAO1. Myosin 1B has been studied in relation to neuronitis, amebiasis and dysentery.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Myosin 1B Antibody (NBP1-97778)(1)


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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MYO1B
Entrez
Uniprot