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MMP-9 Antibody (4A3) [Janelia Fluor® 669]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC
Clone
4A3
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 669

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-13173AF350
Alexa Fluor 405
NBP2-13173AF405
Alexa Fluor 488
NBP2-13173AF488
Alexa Fluor 532
NBP2-13173AF532
Alexa Fluor 594
NBP2-13173AF594
Alexa Fluor 647
NBP2-13173AF647
Alexa Fluor 700
NBP2-13173AF700
Alexa Fluor 750
NBP2-13173AF750
Allophycocyanin
NBP2-13173APC
Biotin
NBP2-13173B
CoraFluor 1
NBP2-13173CL1
DyLight 350
NBP2-13173UV
DyLight 405
NBP2-13173V
DyLight 488
NBP2-13173G
DyLight 550
NBP2-13173R
DyLight 594
NBP2-13173DL594
DyLight 650
NBP2-13173C
DyLight 680
NBP2-13173FR
DyLight 755
NBP2-13173IR
FITC
NBP2-13173F
HRP
NBP2-13173H
Janelia Fluor 525
NBP2-13173JF525
Janelia Fluor 549
NBP2-13173JF549
Janelia Fluor 646
NBP2-13173JF646
PE
NBP2-13173PE
PerCP
NBP2-13173PCP
Unconjugated
NBP2-13173
mFluor Violet 450 SE
NBP2-13173MFV450
mFluor Violet 500 SE
NBP2-13173MFV500
mFluor Violet 610 SE
NBP2-13173MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-13173
Azide and BSA Free
NBP2-80855
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MMP-9 Antibody (4A3) [Janelia Fluor® 669] Summary

Immunogen
Synthetic peptide from the N-terminus of human MMP9 [Swiss-Prot# P14780]
Specificity
Active MMP9. It does not react with the MMP9 proenzyme or the active and proenzyme forms of MMP2.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MMP9
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID:32725911). Mouse reactivity reported in scientific literature (PMID: 30772382). .

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for MMP-9 Antibody (4A3) [Janelia Fluor® 669]

  • 92 kDa gelatinase
  • 92 kDa type IV collagenase
  • CLG4B
  • EC 3.4.24
  • EC 3.4.24.35
  • Gelatinase B
  • GELB
  • macrophage gelatinase
  • MANDP2
  • matrix metallopeptidase 9
  • matrix metalloproteinase 9
  • matrix metalloproteinase-9
  • MMP9
  • MMP-9
  • type V collagenase

Background

Matrix metalloproteinases (MMPs) are zinc-dependent endopeptidases that degrade substances within the extracellular matrix. The MMP family includes six different groups of enzymes: collagenases, gelatinases, stromelysins, transmembrane MMPs, matrilysins and others. MMPs are secreted as proenzymes that have to be cleaved in order to be activated. Other MMPs, plasmins as well as other factors activate MMPs. MMPs are thought to play an important role in tissue remodeling associated with various physiological and pathological processes. MMP9 degrades of proteins in the extracellular matrix and activates growth factors like proTGF beta and proTNF alpha. MMP9 contributes to the invasion and metastasis of various human malignancies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for MMP-9 Antibody (NBP2-13173JF669). (Showing 1 - 1 of 1 FAQ).

  1.  I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any? 
    • We have 10 primary antibodies for MMP-9 that have been tested in ELISA, seen here.It looks like 2 have been tested for capture (please note the tested species for each of these), seen here.6 have been tested for detection, seen here.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MMP9