MED7 Antibody (PCRP-MED7-1B8) - Azide and BSA Free Summary
Description |
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13816).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
Immunogen |
Recombinant full-length human MED7 protein (Uniprot: O43513) |
Localization |
Nucleus, Nuclear bodies |
Isotype |
IgG2b |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
MED7 |
Purity |
Protein A or G purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- ELISA
- Flow Cytometry 1-2 ug/million cells
- Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
- Immunoprecipitation 1-2 ug per 100-500 ug protein
- Protein Array
|
Application Notes |
ELISA: use assay dependent concentration
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate) |
Theoretical MW |
27.2 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at -20 to -80C. Avoid freeze-thaw cycles. |
Buffer |
10mM PBS |
Preservative |
No Preservative |
Concentration |
1 mg/ml |
Purity |
Protein A or G purified |
Alternate Names for MED7 Antibody (PCRP-MED7-1B8) - Azide and BSA Free
Background
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Two transcript variants encoding the same protein have been found for this gene.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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