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mCherry Antibody (2783C) [Unconjugated]

Images

 
Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with mVISTA. PVDF membrane was probed with 0.25 µg/mL of Rabbit Anti-mCherry Monoclonal Antibody (Catalog # ...read more
mCherry was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with mCherry using Rabbit Anti-mCherry Monoclonal Antibody (Catalog # MAB11041) at 3 µg/mL for 3 hours at room ...read more
HEK293 human cell line transfected with mCherry (filled histogram) or EGFP (open histogram) was stained with Rabbit Anti-mCherry Monoclonal Antibody (Catalog # MAB11041), followed by Allophycocyanin-conjugated ...read more
Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with mVISTA, loaded at 0.2 mg/mL. Specific bands were detected for mCherry at approximately 36 and ...read more

Product Details

Summary
Reactivity All-MultiSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF
Clone
2783C
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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mCherry Antibody (2783C) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Human mCherry synthetic peptide
Specificity
Detects human mCherry in direct ELISAs.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 3-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Simple Western 25 ug/mL
  • Western Blot 0.25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for mCherry Antibody (2783C) [Unconjugated]

  • DSRED
  • mCherry
  • red fluorescent protein mCherry
  • Red Fluoroscent Protein

Background

mCherry is a member of the mFruits family of monomeric red fluorescent proteins. It was derived from DsRed of Discoscoma sea anemones. mFruit are second-generation monomeric red fluorescent proteins that have improved brightness and photostability compared to first-generation proteins. The gene for mCherry is 711bp long and the protein is made up of 236 residues with a mass of 76.722 kDa. mCherry is used in fluorescence microscopy as an intracellular probe where constitutive gene expression is desired and other experimental approaches require coordinated control of multiple genes.
  1. Shaner, N.C. Campbell, R.E. Steinbach, P.A. Giepmans B.N.G. Palmer A.E. Tsien, R Y. "Improved Monomeric Red, Orange and Yellow Fluorescent Proteins Derived from Discomsoma Sp. Red Fluorescent Protein" Nature Biotechnology. 22(12):1567. 2004 Nov. 21.
  2. Shu, X. Shaner N C. Yarbrough, C.A. Tsien, R Y. Remington S.J. "Novel Chromophores and Buried Charges Control Color in mFruits" Biochemistry. 45(32):9639. 2006 Aug.
  3. Gebhardt, M.J. Jacobson, R.K. Shuman, H. "Seeing Red; The Development of pON mCherry, a Broad-host Range Constitutive Expression Plasmid for Gram-negative Bacteria" POS ONE. 12(3):e173116. 2017 Mar. 3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for mCherry Antibody (MAB11041) (0)

There are no publications for mCherry Antibody (MAB11041).
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for mCherry Antibody (MAB11041). (Showing 1 - 1 of 1 FAQ).

  1. Does this antibody cross-react with GFP epitopes? As I would like to use both GFP and mCherry antibodies during histochemistry I would not like them to cross-react.
    • mCherry and GFP share just 29% sequence similarity, so this antibody is not predicted to cross-react to GFP and has never shown any ability to detect GFP in testing.

Secondary Antibodies

 

Isotype Controls

Additional mCherry Products

Array MAB11041

Blogs on mCherry.

Successful Transplantation of Friedreich Ataxia Induced Pluripotent Stem Cell (iPSC)-Derived Sensory Neurons in Dorsal Root Ganglia of Adult Rodents
Jamshed Arslan, Pharm D, PhD The dorsal root ganglia (DRG) are a collection of cell bodies of sensory nerves carrying sensory information – including nociception, mechanoreception and proprioception – from periphera...  Read full blog post.

Autophagy and RAS signaling: Clinical implications
By Christina Towers, PhD The cellular recycling process known as autophagy is currently being targeted in over 60 clinical trials focused on treating different types of cancer1. To date, the only autophagy-targeted ...  Read full blog post.


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How to visualize autophagy by microscopy
By Christina Towers, PhD Autophagy is a recycling process that relies on the formation of a unique organelle termed an autophagosome. An elegant way to monitor autophagy is through various microscopy techniques to...  Read full blog post.


  Read full blog post.

Animal Models to Study Autophagy
By Christina Towers, PhD What is autophagy?Autophagy is the catabolic process that degrades cytoplasmic material via the lysosome. The process of macroautophagy was originally characterized in yeast, where the...  Read full blog post.

Application Focus: I see an increase in LC3, now what?
 By Christina Towers, PhD.  Autophagy is highly conserved and tightly regulated process that all cell types use to recycle nutrients, particularly in the instance of stress1. As a result, even sm...  Read full blog post.

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