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Map17 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: Map17 Antibody [NBP1-84290] - Analysis in human kidney and endometrium tissues. Corresponding Map17 RNA-seq data are presented for the same tissues.
Western Blot: Map17 Antibody [NBP1-84290] - Analysis in control (vector only transfected HEK293T lysate) and PDZK1IP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T ...read more
Immunocytochemistry/ Immunofluorescence: Map17 Antibody [NBP1-84290] - Staining of human cell line RPTEC TERT1 shows localization to nuclear speckles & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Map17 Antibody [NBP1-84290] - Staining of human skin shows weak membranous and cytoplasmic positivity in superficial layer of squamous epithelial cells.
Immunohistochemistry-Paraffin: Map17 Antibody [NBP1-84290] - Staining of human endometrium shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: Map17 Antibody [NBP1-84290] - Staining of human kidney shows strong positivity in apical membrane in cells in tubules.
Immunohistochemistry-Paraffin: Map17 Antibody [NBP1-84290] - Staining of human lymphoid tissues shows moderate cytoplasmic positivity in non-germinal center cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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Map17 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: QEEPEPAHMILTVGNKADGVLVGTDGRYSSMAASFRSSEHENAYENVPEEEGKVRST
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDZK1IP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Western Blot 0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Map17 Protein (NBP1-84290PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Map17 Antibody

  • 17 kDa membrane-associated protein
  • DD96
  • MAP17epithelial protein up-regulated in carcinoma, membrane associated protein 17
  • membrane-associated protein 17
  • PDZK1 interacting protein 1
  • PDZK1-interacting protein 1
  • Protein DD96
  • RP1-18D14.5
  • SPAP

Background

Map17, also known as small PDZK1-associated protein (SPAP) is an endogenous regulator of cellular PDZK1 levels through the regulation of PDZK1 turnover. MAP17 is only expressed at significant levels in the proximal tubular epithelial cells of the kidney, and is diffusely expressed in various carcinomas originating from kidney, colon, lung and breast. MAP17 is thought to be associates with tumor formation, and MAP17 antibodies are useful for cancer research and protein turnover studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Research Areas for Map17 Antibody (NBP1-84290)

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Blogs on Map17.

One MAP to Navigate the Oxidative Stress, Tumorigenesis and Apoptosis Pathways?
Reactive oxygen species, ROS, are beneficially involved in many signaling pathways that control development and maintain cellular homeostasis. In physiological conditions, a tightly regulated redox balance protects cells from injurious ROS activity, a...  Read full blog post.

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Bioinformatics

Gene Symbol PDZK1IP1