L3MBTL4 Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

L3MBTL4, also known as Lethal(3)malignant brain tumor-like protein 4, has a 623 amino acid isoform that is 71 kDa and a short 534 amino acid that is 61 kDa, nucleus located; belongs to Putative Polycomb group (PcG), and maintains the transcriptionally repressive state of genes, probably via a modification of chromatin, rendering it heritably changed in its expressibility. There has been research revolving around the L3MBTL4 protein involvement in breast cancer. This protein is involved in transcription, DNA-dependent; regulation of transcription, DNA-dependent; and chromatin modification pathways.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Array NBP2-05137

• L3MBTL4 Antibodies
• L3MBTL4 Lysate
• L3MBTL4 Proteins