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IRF2 Antibody (3B5)

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Western Blot: IRF2 Antibody (3B5) [H00003660-M02] - Analysis of IRF2 expression in transfected 293T cell line by IRF2 monoclonal antibody (M02), clone 3B5.Lane 1: IRF2 transfected lysate(39.4 KDa).Lane 2: Non-transfected lysate.
Western Blot: IRF2 Antibody (3B5) [H00003660-M02] - Analysis of IRF2 expression in transfected 293T cell line by IRF2 monoclonal antibody (M02), clone 3B5.Lane 1: IRF2 transfected lysate(39.4 KDa).Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: IRF2 Antibody (3B5) [H00003660-M02] - Analysis of monoclonal antibody to IRF2 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: IRF2 Antibody (3B5) [H00003660-M02] - Analysis of monoclonal antibody to IRF2 on formalin-fixed paraffin-embedded human leiomyosarcoma. Antibody concentration 3 ug/ml.
Western Blot: IRF2 Antibody (3B5) [H00003660-M02] - IRF2 monoclonal antibody (M02), clone 3B5 Analysis of IRF2 expression in Hela S3 NE.
Sandwich ELISA: IRF2 Antibody (3B5) [H00003660-M02] - Detection limit for recombinant GST tagged IRF2 is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
3B5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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IRF2 Antibody (3B5) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
IRF2 (NP_002190, 216 a.a. ~ 315 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSELYPLQISPVSSYAESETTDSVPSDEESAEGRPHWRKRNIEGKQYLSNMGTRGSYLLPGMASFVTSNKPDLQVTIKEESNPVPYNSSWPPFQDLPLSS
Localization
Nuclear
Specificity
IRF2 - interferon regulatory factor 2
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
IRF2
Purity
IgG purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for IRF2 Antibody (3B5)

  • DKFZp686F0244
  • interferon regulatory factor 2
  • IRF2
  • IRF-2

Background

IRF2 encodes interferon regulatory factor 2, a member of the interferon regulatory transcription factor (IRF) family. IRF2 competitively inhibits the IRF1-mediated transcriptional activation of interferons alpha and beta, and presumably other genes that employ IRF1 for transcription activation. However, IRF2 also functions as a transcriptional activator of histone H4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IRF2
Entrez
OMIM
Uniprot