Reactivity | HuSpecies Glossary |
Applications | WB, B/N |
Clonality | Polyclonal |
Host | Goat |
Conjugate | Unconjugated |
Concentration | LYOPH |
Immunogen | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑4 R alpha Gly24-His232 Accession # P24394 |
Specificity | Detects human IL‑4 R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) IL‑5 R alpha , rhIL-5 R beta , rhIL-9 R, rhIL-13 R alpha 1, rhIL-13 R alpha 2 and recombinant mouse IL-4 R is observed. |
Source | N/A |
Isotype | IgG |
Clonality | Polyclonal |
Host | Goat |
Gene | IL4R |
Purity Statement | Protein A or G purified |
Endotoxin Note | <0.10 EU per 1 μg of the antibody by the LAL method. |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
|
|
Publications |
|
Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Preservative | No Preservative |
Concentration | LYOPH |
Reconstitution Instructions | Reconstitute at 1 mg/mL in sterile PBS. |
Interleukin 4 (IL-4) is a pleiotropic cytokine produced by activated T cells, mast cells, and basophils. The biological functions of IL-4 are mediated by the binding of IL‑4 to high-affinity cell surface receptor complexes. Two types of IL-4 receptor complexes have been described. The type I IL-4 receptor complex is composed of a high-affinity IL-4-binding subunit (referred to as IL-4 R alpha ) and the common gamma chain that does not bind IL-4 by itself. The type II IL-4 receptor complex is composed of IL-4 R alpha and IL-13 R alpha 1. Besides IL-4 signals, the type II IL-4 receptor complex can also transduce IL-13 signals. In the type II complex, the IL-4 R alpha subunit binds only IL-4 and not IL-13. Similarly, the IL-13 R alpha 1 subunit binds only IL-13 and not IL-4. The cDNA clones for both the human and mouse IL-4 R alpha have been isolated and shown to encode an approximately 140 kDa type I transmembrane protein with a large cytoplasmic domain that is essential for signal transduction. In mouse cells, an alternatively spliced variant encoding a soluble secreted IL-4 R alpha isoform has also been identified. Naturally occurring soluble IL-4 R alpha that binds IL-4 with high-affinity has been found in mouse and human biological fluids.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.