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IL-12 p70/IL-12A Antibody [CoraFluor™ 1]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-98685AF350
Alexa Fluor 405
NBP2-98685AF405
Alexa Fluor 488
NBP2-98685AF488
Alexa Fluor 532
NBP2-98685AF532
Alexa Fluor 594
NBP2-98685AF594
Alexa Fluor 647
NBP2-98685AF647
Alexa Fluor 700
NBP2-98685AF700
Alexa Fluor 750
NBP2-98685AF750
Allophycocyanin
NBP2-98685APC
Biotin
NBP2-98685B
DyLight 350
NBP2-98685UV
DyLight 405
NBP2-98685V
DyLight 488
NBP2-98685G
DyLight 550
NBP2-98685R
DyLight 594
NBP2-98685DL594
DyLight 650
NBP2-98685C
DyLight 680
NBP2-98685FR
DyLight 755
NBP2-98685IR
FITC
NBP2-98685F
HRP
NBP2-98685H
Janelia Fluor 525
NBP2-98685JF525
Janelia Fluor 549
NBP2-98685JF549
Janelia Fluor 646
NBP2-98685JF646
Janelia Fluor 669
NBP2-98685JF669
PE
NBP2-98685PE
PerCP
NBP2-98685PCP
Unconjugated
NBP2-98685-100ul
mFluor Violet 450 SE
NBP2-98685MFV450
mFluor Violet 500 SE
NBP2-98685MFV500
mFluor Violet 610 SE
NBP2-98685MFV610
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IL-12 p70/IL-12A Antibody [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Produced in rabbits immunized with purified, a synthetic peptide corresponding to the N-terminus of the mouse IL-12 p70/IL-12A.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IL12A
Purity
Antigen and protein A Affinity-purified
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Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Antigen and protein A Affinity-purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for IL-12 p70/IL-12A Antibody [CoraFluor™ 1]

  • CLMF p35
  • Cytotoxic lymphocyte maturation factor 35 kDa subunit
  • IL12 p70
  • IL-12 p70
  • IL-12 subunit p35
  • IL-12, subunit p35
  • interleukin 12, p35
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35)
  • interleukin-12 alpha chain
  • interleukin-12 subunit alpha
  • natural killer cell stimulatory factor 1, 35 kD subunit
  • NF cell stimulatory factor chain 1
  • NK cell stimulatory factor chain 1
  • NKSF1
  • p35

Background

Interleukin-12, or IL-12, also known as natural killer cell stimulatory factor (NKSF) and cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine that affects both natural killer (NK) cells and T lymphocytes. IL-12 is a heterodimer of subunit IL-12A p35 and subunit IL-12B p40 that is secreted by a wide variety of antigen presenting cells (APCs), including phagocytes, B cells and Langerhans cells. It is involved in mediating many aspects of antiviral immunity. In addition to stimulating T helper cell development, IL-12 is a potent inducer of interferon-gamma (IFN-gamma) production by T cells and NK cells. Increased IL-12 production is a consequence of EBV, CMV, LCMV or HSV infection. While it is unclear what separate effects can be attributed to each IL-12 subunit, evidence suggests IL-12A p35 is the biologically active component responsible for signal transduction via the IL-12 receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IL12A