Home » IL-12/IL-35 p35 » IL-12/IL-35 p35 Antibodies » IL-12/IL-35 p35 Antibody (116219) [DyLight 350]
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IL-12/IL-35 p35 Antibody (116219) [DyLight 350]

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Product Details

Summary
Reactivity PoSpecies Glossary
Applications WB, CyTOF-ready, Flow
Clone
116219
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 350

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC1171U-100UG
Alexa Fluor 405
IC1171V-100UG
Alexa Fluor 488
IC1171G-100UG
Alexa Fluor 532
FAB1171X
Alexa Fluor 594
IC1171T-100UG
Alexa Fluor 647
IC1171R-100UG
Alexa Fluor 700
IC1171N-100UG
Alexa Fluor 750
IC1171S-100UG
Allophycocyanin
FAB1171A
Biotin
FAB1171B
DyLight 405
FAB1171E
DyLight 488
FAB1171K
DyLight 550
FAB1171L
DyLight 594
FAB1171M
DyLight 650
FAB1171W
DyLight 680
FAB1171Y
DyLight 755
FAB1171Z
FITC
FAB1171F
HRP
FAB1171H
Janelia Fluor 525
FAB1171JF525
Janelia Fluor 549
FAB1171I
Janelia Fluor 646
FAB1171J
Janelia Fluor 669
FAB1171JF669
PE
FAB1171P
PerCP
FAB1171C
Unconjugated
MAB1171
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB1171
Carrier Free
MAB1171
Carrier Free
MAB1171-SP
Carrier Free
IC1171U-100UG
Carrier Free
IC1171T-100UG
Carrier Free
IC1171S-100UG
Carrier Free
IC1171R-100UG
Carrier Free
IC1171N-100UG
Carrier Free
IC1171G-100UG
Carrier Free
IC1171V-100UG
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IL-12/IL-35 p35 Antibody (116219) [DyLight 350] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant porcine IL‑12 p35/p40 heterodimer
p40 Ile23-Asn324, p35 Arg26-Ser222
Accession # Q28938, Q29053
Specificity
Detects the heterodimeric form of porcine IL‑12 (p35/p40) in direct ELISAs. Western blotting data indicates recognition of the IL-12/IL-35 p35 subunit. In direct ELISAs and Western blots, 20‑100% cross-reactivity with the disulfide‑linked heterodomeric forms of recombinant mouse IL‑12 and recombinant human IL-12 is observed.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified from hybridoma culture supernatant

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for IL-12/IL-35 p35 Antibody (116219) [DyLight 350]

  • CLMF p35
  • CLMF
  • cytotoxic lymphocyte maturation factor 1, p35
  • Cytotoxic lymphocyte maturation factor 35 kDa subunit
  • IL-12, subunit p35
  • IL-12/IL-35 p35
  • IL-12A
  • IL35 subunit
  • IL35p35
  • interleukin 12, p35
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
  • interleukin-12 alpha chain
  • interleukin-12 subunit alpha
  • NF cell stimulatory factor chain 1
  • NFSK
  • NK cell stimulatory factor chain 1
  • NKSF1
  • p35

Background

This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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