![Western Blot: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [FITC] [NBP1-73637] - Western blot of Rabbit anti-Mouse IgG2a Heavy Chain Secondary antibody [FITC].Lane 1: Mouse IgG2a.Lane 2: None.Load: 50 ng per lane.Primary antibody: None.Secondary antibody: Rabbit anti-Mouse IgG2a Heavy Chain Secondary antibody [FITC] at 1:1,000 for 60 min at RT.Blocking buffer for 30 min at RT.Predicted/Observed size: 55 kDa, 55 kDa for Mouse IgG2a (Gamma 2a chain).Other band(s): None.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG2a-Heavy-Chain-Secondary-Antibody-[FITC]-Western-Blot-NBP1-73637-img0005.jpg "Western Blot: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [FITC] [NBP1-73637] - Western blot of Rabbit anti-Mouse IgG2a Heavy Chain Secondary antibody [FITC].Lane 1: Mouse IgG2a.Lane 2: None.Load: 50 ng per lane.Primary antibody: None.Secondary antibody: Rabbit anti-Mouse IgG2a Heavy Chain Secondary antibody [FITC] at 1:1,000 for 60 min at RT.Blocking buffer for 30 min at RT.Predicted/Observed size: 55 kDa, 55 kDa for Mouse IgG2a (Gamma 2a chain).Other band(s): None.")
| Reactivity | MuSpecies Glossary |
| Applications | WB, DB, ELISA, Flow, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | FITC |
| Concentration | LYOPH |
| Description | Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG2a coupled to agarose beads followed by solid phase adsorption(s) against a panel of immobilized mouse and human serum proteins resulting in a conjugated antibody designed for flow cytometry |
| Immunogen | Mouse IgG2a heavy chain |
| Specificity | Specificity was confirmed by ELISA. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Multi-step |
| Dilutions |
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| Application Notes | This product has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. |
| Storage | Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% Sodium Azide |
| Concentration | LYOPH |
| Purity | Multi-step |
| Reconstitution Instructions | Reconstitute with 1.0 ml deionized water (or equivalent). |
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![Dot Blot: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [FITC] [NBP1-73637]](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG2a-Heavy-Chain-Secondary-Antibody-[FITC]-Dot-Blot-NBP1-73637-img0004.jpg "Dot Blot: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [FITC] [NBP1-73637]")
![Dot Blot results of Rabbit Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [FITC]. Dots are Mouse IgG2a at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. blocking for 30 min at RT. Primary Antibody: Rabbit Anti-Mouse IgG2a FITC at 1ug/mL for 1hr at RT. Secondary Antibody: none. Imaged with BioRad ChemiDoc, FITC filter.](http://images.novusbio.com/fullsize/nbp1-73637_rabbit-polyclonal-rabbit-anti-mouse-igg2a-heavy-chain-secondary-antibody-fitc-23520231750166.jpg "Dot Blot results of Rabbit Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [FITC]. Dots are Mouse IgG2a at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. blocking for 30 min at RT. Primary Antibody: Rabbit Anti-Mouse IgG2a FITC at 1ug/mL for 1hr at RT. Secondary Antibody: none. Imaged with BioRad ChemiDoc, FITC filter.")
