![Western Blot: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [Alkaline Phosphatase] [NBP1-72673] - Western Blot of Rabbit anti-Mouse IgG2a Heavy Chain Secondary antibody [Alkaline Phosphatase].Lane 1: Mouse IgG2a.Lane 2: none. Load: 50ng per lane.Primary antibody: none.Secondary antibody: Rabbit anti-Mouse IgG2a Heavy Chain Secondary antibody [Alkaline Phosphatase] at 1:1,000 at 4C.Block for 30 min at RT.Reaction visualized with alkaline phosphatase substrate for 30 seconds at RT.Predicted/Observed size: 55kDa for Mouse IgG2a.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG2a-Heavy-Chain-Secondary-Antibody-[Alkaline-Phosphatase]-Western-Blot-NBP1-72673-img0006.jpg "Western Blot: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [Alkaline Phosphatase] [NBP1-72673] - Western Blot of Rabbit anti-Mouse IgG2a Heavy Chain Secondary antibody [Alkaline Phosphatase].Lane 1: Mouse IgG2a.Lane 2: none. Load: 50ng per lane.Primary antibody: none.Secondary antibody: Rabbit anti-Mouse IgG2a Heavy Chain Secondary antibody [Alkaline Phosphatase] at 1:1,000 at 4C.Block for 30 min at RT.Reaction visualized with alkaline phosphatase substrate for 30 seconds at RT.Predicted/Observed size: 55kDa for Mouse IgG2a.")
| Reactivity | MuSpecies Glossary |
| Applications | WB, DB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Alkaline Phosphatase |
| Description | Store vial at 4C before opening. DO NOT FREEZE. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Rabbit Serum |
| Immunogen | Mouse IgG2a heavy chain |
| Specificity | Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Multi-step |
| Dilutions |
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| Application Notes | This product has been tested by ELISA, dot blot, and western blot This antibody is assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 is suggested for this product. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.1% Sodium Azide |
| Purity | Multi-step |
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![Dot Blot: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [Alkaline Phosphatase] [NBP1-72673]](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG2a-Heavy-Chain-Secondary-Antibody-[Alkaline-Phosphatase]-Dot-Blot-NBP1-72673-img0005.jpg "Dot Blot: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [Alkaline Phosphatase] [NBP1-72673]")
![Dot Blot of Mouse IgG2a Antibody Alkaline Phosphatase Conjugated. Antigen: Mouse IgG2a Load: Lane 1 - 200ng Lane 2 - 66.7ng Lane 3 - 22.2ng Lane 4 - 7.4 Lane 2.5ng Primary antibody: none Secondary antibody: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [Alkaline Phosphatase]at 1:1,000 for 60 min at RT. Block for 60 min at RT Reaction visualized using alkaline phosphatase substrate for 30 seconds at RT.](http://images.novusbio.com/fullsize/nbp1-72673_rabbit-polyclonal-rabbit-anti-mouse-igg2a-heavy-chain-secondary-antibody-alkaline-phosphatase-235202317492117.jpg "Dot Blot of Mouse IgG2a Antibody Alkaline Phosphatase Conjugated. Antigen: Mouse IgG2a Load: Lane 1 - 200ng Lane 2 - 66.7ng Lane 3 - 22.2ng Lane 4 - 7.4 Lane 2.5ng Primary antibody: none Secondary antibody: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [Alkaline Phosphatase]at 1:1,000 for 60 min at RT. Block for 60 min at RT Reaction visualized using alkaline phosphatase substrate for 30 seconds at RT.")