![ELISA: Rat IgG2a ELISA Kit (Colorimetric) [NBP3-00475] - Samples were spiked with high concentrations of Rat IgG2a and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-00475_rat-igg2a-elisa-kit-colorimetric-132202416133644.jpg "ELISA: Rat IgG2a ELISA Kit (Colorimetric) [NBP3-00475] - Samples were spiked with high concentrations of Rat IgG2a and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
| Reactivity | RtSpecies Glossary |
| Applications | ELISA |
| Suitable Sample Type | Serum, plasma, cell supernatant, and other biological fluids |
| Standard Curve Range | 6.25 - 400 ng/mL |
| Sensitivity | 3.75 ng/mL |
| Standard Curve Range | 6.25 - 400 ng/mL |
| Sensitivity | 3.75 ng/mL |
| Assay Type | Sandwich-ELISA |
| Inter-Assay | %CV < 6.68 |
| Intra-Assay | %CV < 5.01 |
| Spike Recovery | 86 - 100% |
| Sample Volume | 100 uL |
| Kit Type | ELISA Kit (Colorimetric) |
| Storage | Storage of components varies. See protocol for specific instructions. |
| Components |
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![ELISA: Rat IgG2a ELISA Kit (Colorimetric) [NBP3-00475] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-00475_rat-igg2a-elisa-kit-colorimetric-4320249133250.jpg "ELISA: Rat IgG2a ELISA Kit (Colorimetric) [NBP3-00475] - Standard Curve Reference")
