| Reactivity | MuSpecies Glossary |
| Applications | ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Sheep |
| Conjugate | FITC |
| Concentration | LYOPH |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP2-69567 | |||
| Biotin | NBP2-69570 | |||
| FITC | NBP2-69568 | |||
| FITC | NBP2-69573 | |||
| HRP | NBP2-69572 | |||
| TRITC | NBP2-69565 | |||
| Unconjugated | NBP2-69579 | |||
| Description | The lyophilized immunoconjugate is shipped at ambient temperature and may be stored at 4C; prolonged storage at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. |
| Immunogen | Highly purified normal homogenous IgG2a(1b) isolated from mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure. |
| Specificity | The reactivity of the antiserum is restricted to the Fc portion of the IgG2a molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against mouse serum of the allelic type Igh-1b, including C57Bl and SJL/J, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG2a(1b). No precipitation reaction is obtained with purified IgG2a(1a) (Balb/C, CBA, AKR and NZB), other IgG subclasses, IgA, IgG/Fab fragments or other serum proteins. |
| Clonality | Polyclonal |
| Host | Sheep |
| Purity | Ion exchange chromatography |
| Dilutions |
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| Application Notes | (In)direct immunofluorescence. |
| Storage | Store at 4C in the dark. |
| Buffer | Lyophilized from PBS (pH 7.2) |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Ion exchange chromatography |
| Reconstitution Instructions | Reconstitute by by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20C. |
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