Rat anti-Mouse IgG2a Secondary Antibody (344701) [Unconjugated] Summary
| Immunogen |
Cocktail of mouse IgG1 and IgG2A |
| Specificity |
The specificity of this antibody was confirmed by direct ELISA on mouse IgG2A monoclonal antibodies, and it does not cross-react with IgG1, IgG2B, IgG3, IgM, IgA, or IgE antibodies. By flow cytometry, this isotype-specific secondary antibody detects primary antibodies of the mouse IgG2A isotype, and does not detect primary antibodies of mouse IgG1, IgG2B, or IgG3 class. |
| Source |
N/A |
| Isotype |
IgG1 |
| Clonality |
Monoclonal |
| Host |
Rat |
| Purity Statement |
Protein A or G purified from hybridoma culture supernatant |
Applications/Dilutions
| Dilutions |
- Flow Cytometry 0.25 ug/10^6 cells
|
Packaging, Storage & Formulations
| Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
|
| Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution Instructions |
Reconstitute at 0.5 mg/mL in sterile PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Rat anti-Mouse IgG2a Secondary Antibody (344701) [Unconjugated]
Background
IgG is the most abundant of the five classes of mammalian immunoglobulins in serum. IgG molecules (~150 kDa) consist of two ~50 kDa heavy chains and two 25 kDa light chains connected by disulfide bonds. Antibody specificity is conferred by a combination of somatic rearrangement and hypermutation within variable regions. Mouse IgG isotypes IgG1, IgG2a and IgG2b share approximately 64-78% amino acid sequence identity within the heavy chain constant region domains (CH1, CH2 and CH3) and may vary in their interaction with complement, NK and mast cells, induction or inhibition by specific cytokines, and ability to cross the placenta.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are
guaranteed for 1 year from date of receipt.
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