![ELISA: IgG1 Antibody (RM117) [Unconjugated] [NBP2-62028] - ELISA of human immunoglobulins shows NBP2-62028 only reacts to human IgG1. No cross reactivity with Human IgG2, IgG3, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with different immunoglobulins.NBP2-62028 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.](http://images.novusbio.com/fullsize/IgG1-Antibody-RM117-Unconjugated-ELISA-NBP2-62028-img0004.jpg "ELISA: IgG1 Antibody (RM117) [Unconjugated] [NBP2-62028] - ELISA of human immunoglobulins shows NBP2-62028 only reacts to human IgG1. No cross reactivity with Human IgG2, IgG3, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with different immunoglobulins.NBP2-62028 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA, Flow, ICC/IF, IHC, ELISA |
| Clone | RM117 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Peptide corresponding to the hinge region of Human IgG1 |
| Specificity | This antibody reacts to the heavy chain of human IgG1. It does not cross react to any other IgG subclasses (IgG2, IgG3, or IgG4), and shows no cross reactivity to IgM, IgA, IgD, IgE. This antibody does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | 50% Glycerol/PBS, 1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
| Publication using NBP2-62028 | Applications | Species |
|---|---|---|
| Gobin J Assessment of High Purity Mesenchymal Stromal Cells Derived Extracellular Vesicles Presenting NRP1 Show Functional Suppression of Activated Immune Cells Thesis 2022-01-01 (IP, Human) | IP | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for IgG1 Antibody (NBP2-62028)Find related products by research area.
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Read full blog post. |
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CD16 - Find me on macrophages, neutrophils and NK cells CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells.... Read full blog post. |
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![ELISA: IgG1 Antibody (RM117) [Unconjugated] [NBP2-62028] - Sandwich ELISA using NBP2-62028 as the capture antibody, and biotinylated anti-human light chains (kappa+lambda) antibody NBP2-77396 as the detection antibody, followed by an AP conjugated streptavidin.](http://images.novusbio.com/fullsize/IgG1-Antibody-RM117-Unconjugated-ELISA-NBP2-62028-img0007.jpg "ELISA: IgG1 Antibody (RM117) [Unconjugated] [NBP2-62028] - Sandwich ELISA using NBP2-62028 as the capture antibody, and biotinylated anti-human light chains (kappa+lambda) antibody NBP2-77396 as the detection antibody, followed by an AP conjugated streptavidin.")
![ELISA: IgG1 Antibody (RM117) [Unconjugated] [NBP2-62028] - ELISA showing RM117 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM117 and a monkey IgG bindingantibody (positive control) was used as the detection antibody.](http://images.novusbio.com/fullsize/IgG1-Antibody-RM117-Unconjugated-ELISA-NBP2-62028-img0009.jpg "ELISA: IgG1 Antibody (RM117) [Unconjugated] [NBP2-62028] - ELISA showing RM117 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM117 and a monkey IgG bindingantibody (positive control) was used as the detection antibody.")
![ELISA: IgG1 Antibody (RM117) [NBP2-62028] - Sandwich ELISA for detection of human IgG1 in monkey serum, using NBP2-62028 as the capture antibody and biotinylated-NBP2-77396 as the detection antibody. HRP conjugated streptavidin and TMB were used to yield the colorimetric reaction.](http://images.novusbio.com/fullsize/IgG1-Antibody-RM117-ELISA-NBP2-62028-img0010.jpg "ELISA: IgG1 Antibody (RM117) [NBP2-62028] - Sandwich ELISA for detection of human IgG1 in monkey serum, using NBP2-62028 as the capture antibody and biotinylated-NBP2-77396 as the detection antibody. HRP conjugated streptavidin and TMB were used to yield the colorimetric reaction.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
