Reactivity | HuSpecies Glossary |
Applications | IHC |
Clone | IG1707R |
Clonality | Monoclonal |
Host | Rabbit |
Conjugate | Unconjugated |
Format | Azide and BSA Free |
Concentration | 1 mg/ml |
Description | 1.0 mg/ml of recombinant monoclonal antibody purified by Protein A. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53149). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
Additional Information | Recombinant Monoclonal Antibody. |
Immunogen | Recombinant full-length human IgG Heavy Chain protein |
Localization | Cytoplasm, Cell Surface and Secreted |
Marker | B-Cell Marker |
Specificity | Recognizes a protein of 75kDa, identified as gamma heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. |
Isotype | IgG |
Clonality | Monoclonal |
Host | Rabbit |
Purity | Protein A purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
Theoretical MW | 75 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
Buffer | 10 mM PBS |
Preservative | No Preservative |
Concentration | 1 mg/ml |
Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for IgG Heavy Chain Antibody (NBP2-54449)Find related products by research area.
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