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Goat anti-Human IgG Fc Secondary Antibody [DyLight 488]

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Immunocytochemistry: Goat anti-Human IgG Fc Secondary Antibody [DyLight 488] [NBP1-74980] - SKBR3 human breast cancer cell line. Image from verified customer review.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
DyLight 488
Concentration
LYOPH

Goat anti-Human IgG Fc Secondary Antibody [DyLight 488] Summary

Description
Purity > 95% based on SDS-PAGE. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. After reconstituted, prepare fresh working dilution daily.



Product is stable for up to 4 weeks at 2-8C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20C.
Immunogen
Purified Human IgG, Fc fragment
Specificity
Based on IEP, this antibody reacts with heavy gamma chains on human IgG
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:20 - 1:2000
  • Immunocytochemistry/ Immunofluorescence
  • Immunomicroscopy 1:20 - 1:2000
Reviewed Applications
Read 2 Reviews rated 5
using
NBP1-74980 in the following applications:

Publications
Read Publication using NBP1-74980.

Reactivity Notes

Based on IEP, no reactivity is observed to non-immunoglobulin human serum immunoglobulins and light chains on all human immunoglobulins

Packaging, Storage & Formulations

Storage
Store lyophilized antibody at 4C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20C or below for long term storage.
Buffer
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative
0.05% Sodium Azide
Concentration
LYOPH
Purity
Affinity purified
Reconstitution Instructions
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG Fc Antibody (NBP1-74980)(1)

Reviews for IgG Fc Antibody (NBP1-74980) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NBP1-74980:
Filter by Applications
ICC
(2)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry IgG Fc NBP1-74980
Enlarge
5
reviewed by:
Verified Customer
ICC Human 02/27/2019
View

Summary

ApplicationImmunocytochemistry
Sample TestedHuman breast cancer cell line SKBR3 and this product
SpeciesHuman
Lot19-126-121311
Immunocytochemistry IgG Fc NBP1-74980
Enlarge
5
reviewed by:
Verified Customer
ICC Human 06/28/2018
View

Summary

ApplicationImmunocytochemistry
Sample TestedMDA 468 cell lines
SpeciesHuman
Lot19-126-121311

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for IgG Fc Antibody (NBP1-74980) (0)

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Additional IgG Fc Products

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Verified Customer
02/27/2019
Application: ICC
Species: Human

Verified Customer
06/28/2018
Application: ICC
Species: Human

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