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Goat anti-Human IgG Fc Secondary Antibody

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Immunocytochemistry/ Immunofluorescence: Goat anti-Human IgG Fc Secondary Antibody [NBP1-74974] - SKBR3 human breast cancer cell line. Image from verified customer review. Analysis using the DyLight-550 conjugated ...read more
Immunocytochemistry/ Immunofluorescence: Goat anti-Human IgG Fc Secondary Antibody [NBP1-74974] - MCF7 human breast cancer cell line. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Goat anti-Human IgG Fc Secondary Antibody [NBP1-74974] - SKBR3 human breast cancer cell line. Image from verified customer review.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Func, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Goat anti-Human IgG Fc Secondary Antibody Summary

Description
Purity > 95% based on SDS-PAGE. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Immunogen
Purified Human IgG, Fc fragment
Specificity
Based on IEP, this antibody reacts with heavy gamma chains on human IgG
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Affinity purified

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunoassay
  • Immunocytochemistry/ Immunofluorescence
  • Immunodiffusion
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:100-1:2000
Application Notes
This antibody is suitable for all immunoassay applications.
Publications
Read Publications using
NBP1-74974 in the following applications:

Reactivity Notes

Based on IEP, no reactivity is observed to non-immunoglobulin human serum immunoglobulins and light chains on all human immunoglobulins

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.05% Sodium Azide
Purity
Affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG Fc Antibody (NBP1-74974)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ELISA.


Filter By Application
ELISA
(1)
All Applications
Filter By Species
Human
(1)
All Species
Showing Publications 1 - 2 of 2.
Publications using NBP1-74974 Applications Species
Herranen A. Unraveling the pleomorphic forms of Borrelia burgdorferi Thesis et al Thesis 2014-01-01 (ELISA, Human)

Details:
This reference used the Alkaline Phosphatase version of NBP1-74974.
ELISA Human
Nagahama K, Isomura A, Shimoyamada H et al Membranous nephropathy with masked polyclonal IgG deposits associated with primary Sjögren's syndrome CEN Case Rep 2020-08-10 [PMID: 32770308]

Details:
Citation using the DyLight 488 version of this antibody.

Reviews for IgG Fc Antibody (NBP1-74974) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG Fc Antibody (NBP1-74974) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

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Blogs on IgG Fc

There are no specific blogs for IgG Fc, but you can read our latest blog posts.
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Contact Information

Product PDFs

Calculators

Concentration Calculator

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Bioinformatics