Goat anti-Rat IgG Secondary Antibody [HRP]

Images

 
Western blot shows detection of Rat Anti-Human/Mouse Wnt-5a Monoclonal Antibody (MAB645) using a 1:1000 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under ...read more
Simple Western lane view shows detection of Rat Anti-Mouse S100A9 Monoclonal Antibody ( MAB2065) using a 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted ...read more
Detection of Vimentin in human kidney using 5 ug/mL of Rat monoclonal anti-human Vimentin primary antibody (MAB2105) followed by 30 min incubation with Goat anti Rat HRP conjugated secondary antibodies (Catalog # ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, Simple Western, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
HRP

Order Details

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Catalog# & Conjugate Size Price
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Goat anti-Rat IgG Secondary Antibody [HRP] Summary

Immunogen
Rat IgG
Specificity
Detects rat IgG1, IgG2A, IgG2B heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with human IgG and rabbit IgG is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGHG1
Purity Statement
Antigen Affinity-purified

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Simple Western
  • Western Blot
Publications
Read Publications using
HAF005 in the following applications:

Packaging, Storage & Formulations

Storage
Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied as a 0.2 um filtered solution in Sodium Phosphate and Proclin 300.
Preservative
Proclin 300

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Goat anti-Rat IgG Secondary Antibody [HRP]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgG Antibody (HAF005)(56)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Horse, Transgenic Mouse, Xenograft.

We have publications tested in 4 applications: IHC, IHC-P, Neutralization, Western Blot.


Filter By Application
IHC
(1)
IHC-P
(1)
Neutralization
(1)
Western Blot
(26)
All Applications
Filter By Species
Human
(8)
Mouse
(11)
Rat
(5)
Horse
(1)
Transgenic Mouse
(1)
Xenograft
(1)
All Species
Showing Publications 1 - 10 of 56. Show All 56 Publications.
Publications using HAF005 Applications Species
Guo, T;Pei, F;Zhang, M;Yamada, T;Feng, J;Jing, J;Ho, TV;Chai, Y; Vascular architecture regulates mesenchymal stromal cell heterogeneity via P53-PDGF signaling in the mouse incisor Cell stem cell 2024-04-30 [PMID: 38703771] (Western Blot, Transgenic Mouse) Western Blot Transgenic Mouse
Markus M. Xie, Bingbing Dai, Jason A. Hackney, Tianhe Sun, Juan Zhang, Janet K. Jackman et al. An agonistic anti-signal regulatory protein alpha antibody for chronic inflammatory diseases Cell Reports Medicine 8/15/2023 [PMID: 37490914]
Kunkel, TJ;Townsend, A;Sullivan, KA;Merlet, J;Schuchman, EH;Jacobson, DA;Lieberman, AP; The cholesterol transporter NPC1 is essential for epigenetic regulation and maturation of oligodendrocyte lineage cells Nature communications 2023-07-05 [PMID: 37407594]
Lund, MC;Ellman, DG;Nielsen, PV;Raffaele, S;Fumagalli, M;Guzman, R;Degn, M;Brambilla, R;Meyer, M;Clausen, BH;Lambertsen, KL; Selective Inhibition of Soluble Tumor Necrosis Factor Alters the Neuroinflammatory Response following Moderate Spinal Cord Injury in Mice Biology 2023-06-12 [PMID: 37372129] (Western Blot, Mouse) Western Blot Mouse
H Pazoki, H Mirjalali, M Niyyati, SJ Seyed Taba, N Mosaffa, S Shahrokh, H Asadzadeh, A Kupz, MR Zali Toxoplasma gondii profilin induces NLRP3 activation and IL-1beta production/secretion in THP-1�cells Microbial pathogenesis, 2023-04-18;180(0):106120. 2023-04-18 [PMID: 37080500] (Western Blot, Human) Western Blot Human
H Pazoki, H Mirjalali, M Niyyati, SJ Seyed Taba, N Mosaffa, S Shahrokh, H Asadzadeh, A Kupz, MR Zali Toxoplasma gondii profilin induces NLRP3 activation and IL-1beta production/secretion in THP-1�cells Microbial pathogenesis, 2023;180(0):106120. 2023 [PMID: 37080500] (Western Blot, Human) Western Blot Human
C Wang, R Nistala, M Cao, Y Pan, M Behrens, D Doll, RD Hammer, P Nistala, HM Chang, ETH Yeh, X Kang Dipeptidylpeptidase 4 promotes survival and stemness of acute myeloid leukemia stem cells Cell Reports, 2023-02-17;42(2):112105. 2023-02-17 [PMID: 36807138] (Western Blot, Xenograft) Western Blot Xenograft
M Sándor, FG Thiel, M Schmid, A Demcsák, NC Morales Gr, BC Németh, S Vajda, A Hoerning, M Sahin-Tóth Novel p.G250A Mutation Associated with Chronic Pancreatitis Highlights Misfolding-Prone Region in Carboxypeptidase A1 (CPA1) International Journal of Molecular Sciences, 2022-12-07;23(24):. 2022-12-07 [PMID: 36555104] (Western Blot, Human) Western Blot Human
S Sengupta, S Jati, S Maity, M Sen Wnt5A Signaling Regulates Gut Bacterial Survival and T Cell Homeostasis mSphere, 2022-12-06;7(6):e0050722. 12/6/2022 [PMID: 36472447]
S Nyström, A Kendall, S Adepu, A Lindahl, E Skiöldebra The expression of nerve growth factor in healthy and inflamed equine chondrocytes analysed by capillary western immunoassay Research in Veterinary Science, 2022-08-22;151(0):156-163. 2022-08-22 [PMID: 36029605] (Western Blot, Horse) Western Blot Horse
Show All 56 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for IgG Antibody (HAF005) (0)

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Blogs on IgG.

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CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ...  Read full blog post.

Read our latest blog and use the new citation tool on bio-techne.com

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Bioinformatics

Gene Symbol IGHG1