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Goat anti-Rabbit IgG Secondary Antibody [Biotin]

 (11 Publications)
 
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Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Survivin at approximately 24 kDa (as indicated) using 5 µg/mL (Lane A), 1.25 µg/mL (Lane B), or 0 µg/mL Lane C, negative control) of Rabbit Anti-Human Survivin Antigen Affinity-purified Polyclonal Antibody(Catalog # AF886) followed by 1:500 dilution of Biotin-conjugated Anti-Rabbit IgG Secondary Antibody (BAF008). Streptavidin-HRP was used for detection. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Survivin at approximately 24 kDa (as indicated) using 5 µg/mL ...read more
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP70/HSPA1A at approximately 71 kDa (as indicated) using 5 µg/mL (Lane ...read more

Product Details

Summary
Reactivity RbSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Allophycocyanin
F0111
Fluorescein
F0112
HRP
HAF008
HRP Polymer
VC003-025
HRP Polymer
VC003-050
HRP Polymer
VC003-125
HRP Polymer
VC003-250
Phycoerythrin
F0110
Unconjugated
AF008
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF008
Carrier Free
AF008
Carrier Free
F0110
Carrier Free
F0111
Carrier Free
F0112
Carrier Free
HAF008
Carrier Free
VC003-025
Carrier Free
VC003-050
Carrier Free
VC003-125
Carrier Free
VC003-250
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Goat anti-Rabbit IgG Secondary Antibody [Biotin] Summary

Immunogen
Rabbit IgG
Specificity
Detects rabbit IgG heavy and light chains. In direct ELISAs,  less than 5% cross-reactivity with human IgG, mouse IgG and chicken IgY is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGHG1
Purity Statement
Antigen Affinity-purified

Applications/Dilutions

Dilutions
  • Simple Western 1:500 dilution
  • Western Blot
Publications
Read Publications using
BAF008 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Goat anti-Rabbit IgG Secondary Antibody [Biotin]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgG Antibody (BAF008)(11)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: ELISA Development, Functional Assay, IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
ELISA Development
(2)
Functional Assay
(1)
IHC
(2)
IHC-Fr
(1)
IHC-P
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(5)
Mouse
(2)
Rat
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using BAF008 Applications Species
Humphreys, PEA;Woods, S;Bates, N;Rooney, KM;Mancini, FE;Barclay, C;O'Flaherty, J;Martial, FP;Domingos, MAN;Kimber, SJ; Optogenetic manipulation of BMP signaling to drive chondrogenic differentiation of hPSCs Cell reports 2023-11-28 [PMID: 38032796] (IHC, Human) IHC Human
X He, C Yan, S Zhao, Y Zhao, R Huang, Y Li The preventive effects of probiotic Akkermansia muciniphila on D-galactose/AlCl3 mediated Alzheimer's disease-like rats Experimental gerontology, 2022-09-21;0(0):111959. 2022-09-21 [PMID: 36152776] (IHC, Rat) IHC Rat
Tang Y, Sun X, et al. Inhibition of Ezrin suppresses cell migration and invasion in human nasopharyngeal carcinoma. Oncol Lett 7/1/2019 [PMID: 31289527]
X Zhang, G Shi, F Gao, P Liu, H Wang, X Tan TSPAN1 upregulates MMP2 to promote pancreatic cancer cell migration and invasion via PLC? Oncol. Rep., 2019-01-30;0(0):. 2019-01-30 [PMID: 30720116] (IHC-P, Human) IHC-P Human
Tian J, Zhang R, Piao H et al. Silencing Tspan1 inhibits migration and invasion, and induces the apoptosis of human pancreatic cancer cells Mol Med Rep 9/1/2018 [PMID: 30066932]
F da Silva L, AB da Rocha R, A Hastreiter, A Nogueira-P, E Makiyama, C Colli, RA Fock An insight into the role of magnesium in the immunomodulatory properties of mesenchymal stem cells J. Nutr. Biochem., 2018-02-13;55(0):200-208. 2018-02-13 [PMID: 29554498] (Western Blot, Mouse) Western Blot Mouse
Jun-Ying Ding, Zhi-Hua Wang, Zheng-Zheng Zhang, Xu-Ran Cui, Yan-Ying Hong, Qing-Quan Liu Effects of three IL-15 variants on NCI-H446 cell proliferation and expression of cell cycle regulatory molecules Oncotarget 12/8/2017 [PMID: 29296227]
H Feng, J Wang, J Xu, C Xie, F Gao, Z Li The expression of SIRT1 regulates the metastaticplasticity of chondrosarcoma cells by inducing epithelial-mesenchymal transition Sci Rep, 2017-01-23;7(0):41203. 2017-01-23 [PMID: 28112277] (Western Blot, Human) Western Blot Human
Reinhard M, Frystyk J, Jespersen B, Bjerre M, Christiansen J, Flyvbjerg A, Ivarsen P Effect of hyperinsulinemia during hemodialysis on the insulin-like growth factor system and inflammatory biomarkers: a randomized open-label crossover study. BMC Nephrol, 2013-04-04;14(0):80. 2013-04-04 [PMID: 23557110] (ELISA Development, Functional Assay, Human) ELISA Development, Functional Assay Human
Ma P, Bian F, Wang Z, Zheng X, Chotikavanich S, Pflugfelder SC, Li DQ Human corneal epithelium-derived thymic stromal lymphopoietin links the innate and adaptive immune responses via TLRs and Th2 cytokines. Invest. Ophthalmol. Vis. Sci., 2009-01-17;50(6):2702-9. 2009-01-17 [PMID: 19151401] (IHC-Fr, Human) IHC-Fr Human
Show All 11 Publications.

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Product General Protocols

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Bioinformatics

Gene Symbol IGHG1