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IgG Antibody (IG266) [Allophycocyanin/Cy7]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, MA, CyTOF-ready
Clone
IG266
Clonality
Monoclonal
Host
Mouse
Conjugate
Allophycocyanin/Cy7

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-34648AF350
Alexa Fluor 405
NBP2-34648AF405
Alexa Fluor 488
NBP2-34648AF488
Alexa Fluor 532
NBP2-34648AF532
Alexa Fluor 594
NBP2-34648AF594
Alexa Fluor 647
NBP2-34648AF647
Alexa Fluor 700
NBP2-34648AF700
Alexa Fluor 750
NBP2-34648AF750
Allophycocyanin
NBP2-34648APC
Biotin
NBP2-34648B
CoraFluor 1
NBP2-34648CL1
DyLight 350
NBP2-34648UV
DyLight 405
NBP2-34648V
DyLight 488
NBP2-34648G
DyLight 550
NBP2-34648R
DyLight 594
NBP2-34648DL594
DyLight 650
NBP2-34648C
DyLight 680
NBP2-34648FR
DyLight 755
NBP2-34648IR
FITC
NBP2-34648F
HRP
NBP2-34648H
Janelia Fluor 525
NBP2-34648JF525
Janelia Fluor 549
NBP2-34648JF549
Janelia Fluor 585
NBP2-34648JF585
Janelia Fluor 635
NBP2-34648JF635
Janelia Fluor 646
NBP2-34648JF646
Janelia Fluor 669
NBP2-34648JF669
PE
NBP2-34648PE
PE/Atto594
NBP2-34648PEATT594
PE/Cy5.5
NBP2-34648PECY55
PE/Cy7
NBP2-34648PECY7
Unconjugated
NBP2-34250-0.1mg
mFluor Violet 450 SE
NBP2-34648MFV450
mFluor Violet 500 SE
NBP2-34648MFV500
mFluor Violet 610 SE
NBP2-34648MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-34250-0.1mg
IHC-Prediluted
NBP2-45064
Azide and BSA Free
NBP2-34648-0.1mg
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IgG Antibody (IG266) [Allophycocyanin/Cy7] Summary

Immunogen
Purified human IgG
Localization
Cytoplasm, Cell Surface and Secreted
Marker
B-Cell Marker
Specificity
Recognizes a protein of 75kDa, identified as gamma heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This monoclonal antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin s lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
IGHG1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Protein Array
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for IgG Antibody (IG266) [Allophycocyanin/Cy7]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Blogs on IgG.

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Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.

  Read full blog post.
CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.
Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ...  Read full blog post.
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Bioinformatics

Gene Symbol IGHG1