Reactivity | HuSpecies Glossary |
Applications | IHC |
Clone | IGHA/7567R |
Clonality | Monoclonal |
Host | Rabbit |
Conjugate | Unconjugated |
Format | Azide and BSA Free |
Concentration | 1 mg/ml |
Description | Positive Controls: Daudi, 293T, Raji or hPBL cells. Human tonsil or spleen. Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required. |
Additional Information | Recombinant Monoclonal Antibody |
Immunogen | Full-length native protein (purified) corresponding to Human IgA |
Localization | Cytoplasm. Cell surface. Secreted. |
Specificity | This monoclonal antibody is specific to heavy chain of IgA and shows minimal cross-reaction with heavy chains of other immunoglobulins It is reactive with both IgA1 and IgA2 subclasses of Alpha heavy chain It reacts with the third constant domain (CH3) of the alpha chain of IgA molecules |
Isotype | IgG Kappa |
Clonality | Monoclonal |
Host | Rabbit |
Purity | Protein A or G purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
|
Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
Storage | Store at -20 to -70C. Avoid freeze-thaw cycles. |
Buffer | 10mM PBS |
Preservative | No Preservative |
Concentration | 1 mg/ml |
Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.