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IFN-alpha K Antibody (1009720) [Unconjugated]

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Western blot shows lysates of HEK001 human epidermal keratinocyte cell line and NHEK human normal epidermal keratinocytes. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IFN-alpha 6/IFNA6 Monoclonal Antibody (Catalog # MAB10212) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IFN-alpha 6/IFNA6 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HEK001 human epidermal keratinocyte cell line and NHEK human normal epidermal keratinocytes. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IFN-alpha 6/IFNA6 Monoclonal Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
1009720
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB10212-SP
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IFN-alpha K Antibody (1009720) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human IFN-alpha 6/IFNA6
Leu28-Lys207
Accession # Q90W0
Specificity
Detects human IFN-alpha 6/IFNA6 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
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Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFN-alpha K Antibody (1009720) [Unconjugated]

  • IFNA6
  • IFN-alpha 6
  • IFNalpha K
  • IFN-alpha K
  • IFN-kappa
  • interferon kappa
  • interferon, kappa
  • RP11-27J8.1
  • UNQ6124/PRO20084

Background

Interferon kappa, also known as IFN-kappa, is a 207 aminoacids protein that in humans is encoded by the IFNK gene. IFN-kappa is a member of the type I interferon family which also includes IFN-alpha, -beta, -epsilon, and -omega. Type I interferons are a group of related glycoproteins that play an important role in host defenses against viral infections. Human IFN-kappa has been detected in keratinocytes, monocytes, and monocyte-derived dendritic cells and is reported to have contact-dependent antiviral activity. Human papillomavirus (HPV) 16 oncogene expression, which is necessary for the development of cervical cancer, has been shown to down-regulate human IFN-kappa expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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