![Western Blot: HSPC111 Antibody (1G4) [H00051491-M01] - HSPC111 monoclonal antibody (M01), clone 1G4 Analysis of HSPC111 expression in K-562.](http://images.novusbio.com/fullsize/HSPC111-Antibody-1G4-Western-Blot-H00051491-M01-img0010.jpg "Western Blot: HSPC111 Antibody (1G4) [H00051491-M01] - HSPC111 monoclonal antibody (M01), clone 1G4 Analysis of HSPC111 expression in K-562.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clone | 1G4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | HSPC111 (AAH40106, 1 a.a. ~ 178 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPKAKGKTRRQKFGYSVNRKRLNRNARRKAAPRIECSHIRHAWDHAKSVRQNLAEMGLAVDPNRAVPLRKRKVKAMEVDIEERPKELVRKPYVLNDLEAEASLPEKKGNTLSRDLIDYVRYMVENHGEDYKAMARDEKNYYQDTPKQIRSKINVYKRFYPAEWQDFLDSLQKRKMEVE |
| Specificity | HSPC111 - hypothetical protein HSPC111 |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NOP16 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: HSPC111 Antibody (1G4) [H00051491-M01] - Analysis of monoclonal antibody to HSPC111 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/HSPC111-Antibody-1G4-Immunocytochemistry-Immunofluorescence-H00051491-M01-img0006.jpg "Immunocytochemistry/Immunofluorescence: HSPC111 Antibody (1G4) [H00051491-M01] - Analysis of monoclonal antibody to HSPC111 on HeLa cell. Antibody concentration 10 ug/ml.")
![Immunohistochemistry-Paraffin: HSPC111 Antibody (1G4) [H00051491-M01] - Analysis of monoclonal antibody to HSPC111 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/HSPC111-Antibody-1G4-Immunohistochemistry-Paraffin-H00051491-M01-img0008.jpg "Immunohistochemistry-Paraffin: HSPC111 Antibody (1G4) [H00051491-M01] - Analysis of monoclonal antibody to HSPC111 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.")
![Sandwich ELISA: HSPC111 Antibody (1G4) [H00051491-M01] - Detection limit for recombinant GST tagged HSPC111 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HSPC111-Antibody-1G4-Sandwich-ELISA-H00051491-M01-img0009.jpg "Sandwich ELISA: HSPC111 Antibody (1G4) [H00051491-M01] - Detection limit for recombinant GST tagged HSPC111 is approximately 1ng/ml as a capture antibody.")
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")