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hnRNP AB Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: hnRNP AB Antibody [NBP2-16865] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: hnRNP A/B protein stained by hnRNP A/B antibody diluted at 1:500. Blue: ...read more
Immunohistochemistry-Paraffin: hnRNP AB Antibody [NBP2-16865] - A549 xenograft, using HNRNPAB antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Genetic Strategies: Knockout Validated: hnRNP AB Antibody [NBP2-16865] - Wild-type (WT) and hnRNP AB knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ...read more
Immunohistochemistry-Whole-Mount : hnRNP AB Antibody [NBP2-16865] -Fixed zebrafish embryos. hnRNP AB antibody dilution: 1:200.
Western Blot: hnRNP AB Antibody [NBP2-16865] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE NBP2-16865 diluted at 1:1000
Western Blot: hnRNP AB Antibody [NBP2-16865] - Zebrafish tissue extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP AB antibody (NBP2-16865) diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Ze, Mu, Rt, Po, Bv, Ch, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IP, ChIP, IHC, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

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hnRNP AB Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human hnRNP AB. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Mouse (97%), Rat (97%), Porcine (100%), Bovine (98%), Rhesus Macaque (100%), Chicken (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HNRNPAB
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) Assay dependent
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry Whole-Mount Assay dependent
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation Assay dependent
  • Knockout Validated
  • Western Blot 1:500-1:3000
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for hnRNP AB Antibody

  • catalytic polypeptide 1-binding protein 1
  • heterogeneous nuclear ribonucleoprotein A/B
  • hnRNP A/B
  • hnRNP type A/B protein

Background

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are produced by RNA polymerase II and are components of the heterogeneous nuclear RNA (hnRNA) complexes. They are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene, which binds to one of the components of the multiprotein editosome complex, has two repeats of quasi-RRM (RNA recognition motif) domains that bind to RNAs. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HNRNPAB
Uniprot