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HIV-1 gp120 Antibody - BSA Free

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Product Details

Summary
Reactivity V-ViSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

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HIV-1 gp120 Antibody - BSA Free Summary

Immunogen
Purified gp120 from strain IIIB
Specificity
NB100-66437 is specific for gp120, an exterior envelope glycoprotein on the surface of the virus particle that acts as a receptor for CD4 and chemokine receptors on macrophages and T-lymphocytes. It does not react with human T cells, B cells, normal bovine serum or normal human serum. In western blotting a predominant gp120 band is observed.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
env
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
IgG purified

Alternate Names for HIV-1 gp120 Antibody - BSA Free

  • Envelope surface glycoprotein gp120
  • gp120 glycoprotein
  • gp120
  • Human immunodeficiency virus 1
  • SU
  • Surface protein

Background

HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for HIV-1 gp120 Antibody (NB100-66437). (Showing 1 - 1 of 1 FAQ).

  1. We have purchased your product HIV-1 gp120 Antibody, Cat# NB100-66437, can you tell me what's concentration you recommended for IHC fluorescence staining? Thanks.
    • For IHC, the optimal dilution will have to be determined, as samples can vary significantly. For this antibody a starting dilution of 1:100-1:200 should suffice and then it will have to be determined from there.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol env
Uniprot