HIPPI Antibody

Images

 
Western Blot: HIPPI Antibody [NBP1-28913] - Staining of Mouse Brain lysate (35 ug protein in RIPA buffer) with antibody at 1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: HIPPI Antibody [NBP1-28913] - Staining of paraffin embedded Human Testes tissue with antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

HIPPI Antibody Summary

Immunogen
Peptide with sequence C-EQPQEYDDPNATISN corresponding to internal region according to NP_060480.1.
Epitope
internal region
Predicted Species
Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IFT57
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 1 - 3 ug/mL
Application Notes
WB: Approx. 49 kDa band observed in human and mouse brain lysates (calculated MW of 49.1 kDa band according to human NP_060480.1 and of 48.8 kDa band according to mouse NP_082956.2).
Publications
Read Publication using NBP1-28913.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HIPPI Antibody

  • Dermal papilla-derived protein 8
  • DERP8
  • ESRRBL1huntingtin interacting protein-1 interacting protein
  • estrogen-related receptor beta like 1
  • Estrogen-related receptor beta-like protein 1
  • FLJ10147
  • HIPPIHIP1-interacting protein
  • intraflagellar transport 57 homolog (Chlamydomonas)
  • intraflagellar transport protein 57 homolog
  • MHS4R2HIP1 protein interactor

Background

HIPPI is required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway . Has pro-apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and tri

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HIPPI Antibody (NBP1-28913)(1)

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol IFT57
Entrez
Uniprot