Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - Azide and BSA Free Summary
| Immunogen |
Rat Hepatic Sinusoidal Endothelial Cells |
| Isotype |
IgG2a Kappa |
| Clonality |
Monoclonal |
| Host |
Mouse |
| Purity |
Protein A purified |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
| Dilutions |
- Flow Cytometry reported in scientific literature (Motoyama et al; PMID: 9428229)
- Immunocytochemistry/ Immunofluorescence
- Immunohistochemistry 1-5 ug/ml
- Immunohistochemistry-Frozen 1-5 ug/ml
- Immunohistochemistry-Paraffin 1-5 ug/ml
- Western Blot 1-5 ug/ml
|
| Application Notes |
In WB, Hepatic Sinusoidal Endothelial Cells antibody (clone SE-1) generates a specific band around 45-50 kDa molecular weight position. For IHC use neutral buffered formalin fixated (perfusion fixation is recommended) paraffin embedded tissues after proteinase K treatment or acetone fixed frozen sections. |
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer |
Tris-Glycine, 0.15 M NaCl |
| Preservative |
No Preservative |
| Concentration |
1 mg/ml |
| Purity |
Protein A purified |
Alternate Names for Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - Azide and BSA Free
Background
Hepatic sinusoidal endothelial cells (HSEC) plays a key role in various hepatic pathophysiological conditions and are known for their morphological uniqueness - numerous open fenestrations clustered in groups (sieve plates), lack of distinct basal lamina, and abundant vacuolar/vesicular structures. HSECs are distinct from vascular endothelial cells functionally/morphologically and besides providing sinusoidal barrier, they implicates in the balance or distribution of lipids, cholesterol, and vitamin A, and in the uptake of various substances via their specialized endocytotic mechanisms. SE-1 monoclonal antibody has been specifically designed to recognize an antigen expressed in HSEC, but not in other cell types including vascular endothelial cells. Experiments on developing liver have revealed that the expression of SE-1 antigen is possibly regulated via intimate association of HSECs with hepatocytes. Hepatic sinusoidal endothelial cells (SE-1) antibody has been extensively used as one of the most reliable markers for rat HSEC by Western blot and immunohistochemistry analysis and is a useful tool for studying the role of HSE in various hepato-pathophysiological conditions.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Product General Protocols
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Secondary Antibodies
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Blogs on Hepatic Sinusoidal Endothelial Cells
![Western Blot: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - Azide and BSA Free [NBP2-80759] - Total protein from human, mouse and rat liver was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-H.S.E.C in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Hepatic-Sinusoidal-Endothelial-Cells-Antibody-SE-1-Azide-and-BSA-Free-Western-Blot-NBP2-80759-img0001.jpg "Western Blot: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - Azide and BSA Free [NBP2-80759] - Total protein from human, mouse and rat liver was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-H.S.E.C in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - Azide and BSA Free [NBP2-80759] - Release of structural liver cells into the perfusate after cold ischemia. Representative images of surface marker expression of hepatocytes (top), LSEC (middle), and stellate cells (below). Scale bars: 5 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-020-57589-4), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Hepatic-Sinusoidal-Endothelial-Cells-Antibody-SE-1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80759-img0005.jpg "Immunocytochemistry/Immunofluorescence: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - Azide and BSA Free [NBP2-80759] - Release of structural liver cells into the perfusate after cold ischemia. Representative images of surface marker expression of hepatocytes (top), LSEC (middle), and stellate cells (below). Scale bars: 5 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-020-57589-4), licensed under a CC-BY license.")
![Immunohistochemistry-Frozen: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - Azide and BSA Free [NBP2-80759] - Frozen rat liver tissue sections. DAPI (blue), SE1 (green), cytochrome P450 (red). Tissue sections were acetone fixed. SE1 antibody at 1:500, incubated at 4C overnight. Multiplexed with cytochrome P450 from another vendor. IHC-Fr image submitted by a veri](http://images.novusbio.com/fullsize/Hepatic-Sinusoidal-Endothelial-Cells-Antibody-SE-1-Azide-and-BSA-Free-Immunohistochemistry-Frozen-NBP2-80759-img0003.jpg "Immunohistochemistry-Frozen: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - Azide and BSA Free [NBP2-80759] - Frozen rat liver tissue sections. DAPI (blue), SE1 (green), cytochrome P450 (red). Tissue sections were acetone fixed. SE1 antibody at 1:500, incubated at 4C overnight. Multiplexed with cytochrome P450 from another vendor. IHC-Fr image submitted by a veri")
![Flow Cytometry: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - Azide and BSA Free [NBP2-80759] - Using the FITC direct conjugate Flow Cytometry: Surface staining of Rat Liver sinusoidal endothelial cells with Mouse anti-Rat Hepatic Sinusoidal Endothelial Cells [FITC] [NB110-68095F] and negative control. Total viable cells were used for analysis. Image courtesy of customer.](http://images.novusbio.com/fullsize/Hepatic-Sinusoidal-Endothelial-Cells-Antibody-SE-1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80759-img0004.jpg "Flow Cytometry: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - Azide and BSA Free [NBP2-80759] - Using the FITC direct conjugate Flow Cytometry: Surface staining of Rat Liver sinusoidal endothelial cells with Mouse anti-Rat Hepatic Sinusoidal Endothelial Cells [FITC] [NB110-68095F] and negative control. Total viable cells were used for analysis. Image courtesy of customer.")
![Immunohistochemistry: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - Azide and BSA Free [NBP2-80759] - Analysis of frozen normal rat liver tissue sections using anti-Hepatic Sinusoidal Endothelial Cells antibody (green). Nuclei were counterstained with DAPI (blue). Image from verified customer review. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Hepatic-Sinusoidal-Endothelial-Cells-Antibody-SE-1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80759-img0002.jpg "Immunohistochemistry: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - Azide and BSA Free [NBP2-80759] - Analysis of frozen normal rat liver tissue sections using anti-Hepatic Sinusoidal Endothelial Cells antibody (green). Nuclei were counterstained with DAPI (blue). Image from verified customer review. Image from the standard format of this antibody.")
