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HAI-1/HGFA Inhibitor 1 Antibody [Biotin]

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HAI-1 was detected in perfusion fixed frozen sections of mouse intestine using Goat Anti-Mouse HAI-1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1141) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
HAI-1 was detected in perfusion fixed frozen sections of mouse intestine using Goat Anti-Mouse HAI-1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1141) at 15 µg/mL overnight at 4 °C. Tissue ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, ELISA(Det)
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Unconjugated
AF1141
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF1141
Carrier Free
AF1141
Carrier Free
AF1141-SP
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HAI-1/HGFA Inhibitor 1 Antibody [Biotin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse HAI-1
Specificity
Detects mouse HAI‑1 in Western blots. In Western blots, approximately 10% cross‑reactivity with recombinant human (rh) Siglec-7 and rhSiglec-9 is observed and less than 5% cross-reactivity with rhSiglec-2 and rhSiglec-3 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SPINT1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture:Mouse HAI-1 Antibody (Catalog # MAB1141)
Standard: Recombinant Mouse HAI-1 (Catalog # 1141-PI)
Readout System
Publications
Read Publication using
BAF1141 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HAI-1/HGFA Inhibitor 1 Antibody [Biotin]

  • HAI
  • HAI1
  • HAI-1
  • hepatocyte growth factor activator inhibitor 1
  • Hepatocyte growth factor activator inhibitor type 1
  • kunitz-type protease inhibitor 1
  • MANSC2
  • serine peptidase inhibitor, Kunitz type 1
  • serine protease inhibitor, Kunitz type 1
  • SPINT1

Background

Hepatocyte growth factor activator inhibitor-1 (HAI-1) encoded by the Spint1 gene is a Kunitz-type serine protease inhibitor, identified as a strong inhibitor of HGF activator (HGFA) and matripase (1). The membrane-anchored HAI-1 consists of two Kunitz domains, a LDL-receptor-like domain, and a C-terminal transmembrane domain (2). Two soluble forms are generated by ectodomain shedding, one with a single Kunitz domain and the other with two Kunitz domains. HAI-1 is not only an inhibitor but also a specific receptor of active HGFA, acting as a reservoir of this enzyme on the cell surface (3). The shedding of HAI-1 and HGFA/HAI-1 complex is enhanced by treatment with phorbol 12-myristate, 13-acetate or IL-1 beta . The regulated shedding is completely inhibited by a synthetic zinc metalloprotease inhibitor (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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HAI-1/HGFA
Inhibitor 1 Antibody ...
BAF1141
HAI-1/HGFA Inhibitor 1 Antibody [Biotin]
Species: Mu
Applications: WB, IHC, ELISA(Det)

Publications for HAI-1/HGFA Inhibitor 1 Antibody (BAF1141)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC.


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Bioinformatics

Gene Symbol SPINT1