![Immunocytochemistry/Immunofluorescence: HA Tag Antibody [NB600-363] - Detection of HA-tagged Tubulin in CHO cells transfected with mutant beta-tubulin cDNA encoding an HA epitope tag. Photo contributed by R. Bhattacharya and F. Cabral, University of Texas Medical School at Houston.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Immunocytochemistry-Immunofluorescence-NB600-363-img0005.jpg "Immunocytochemistry/Immunofluorescence: HA Tag Antibody [NB600-363] - Detection of HA-tagged Tubulin in CHO cells transfected with mutant beta-tubulin cDNA encoding an HA epitope tag. Photo contributed by R. Bhattacharya and F. Cabral, University of Texas Medical School at Houston.")
| Reactivity | ETSpecies Glossary |
| Applications | WB, ELISA, Flow, GS, ICC/IF, IHC, IP, ChIP, KO |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.1 mg/ml |
| Immunogen | This HA Tag Antibody was developed by immunizing rabbits with HA cleavage site (YPYDVPDYA) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Use in Gel Super Shift Assays reported in scientific literature (PMID:34289349). Use in ChIP reported in (PMID: 30659200).. Use in IHC reported in (PMID: 22880041).. Use in FLOW reported in scientific literature (Pryce R et al).. Knockout validation (PMID: 32354171).. Recommended dilution for coating ELISA plates is 1:100 - 1:500. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.1 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Tanya Kutyavin |
Simple Western | Human | 06/08/2018 |
Summary
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reviewed by:
Filipa Franquinho |
WB | Human | 05/04/2013 |
Summary
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Enlarge |
reviewed by:
Maria Andreou |
WB | Other | 09/26/2011 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for HA Tag Antibody (NB600-363)Find related products by research area.
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Developmental regulator Daam2 promotes glial cell tumors by degrading Von Hippel-Lindau protein By Jamshed Arslan Pharm.D. Glioblastoma is an aggressive type of cancer that forms from the star-shaped glial cells of the central nervous system, called astrocytes. Intriguingly, several genes linked to glioblasto... Read full blog post. |
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| Tanya Kutyavin 06/08/2018 |
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| Application: | Simple Western | |
| Species: | Human |
| Filipa Franquinho 05/04/2013 |
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| Application: | WB | |
| Species: | Human |
| Maria Andreou 09/26/2011 |
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| Application: | WB | |
| Species: | Other |
![Western Blot: HA Tag Antibody [NB600-363] - 200, 100, or 50 ng of E. coli whole cell lysate expressing amulti-tag fusion protein.Antibody: Affinity purified rabbitanti-HA antibody used for WB at 0.04 ug/ml(1:25,000). Detection: Chemiluminescence with anexposure time of 3 seconds.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Western-Blot-NB600-363-img0019.jpg "Western Blot: HA Tag Antibody [NB600-363] - 200, 100, or 50 ng of E. coli whole cell lysate expressing amulti-tag fusion protein.Antibody: Affinity purified rabbitanti-HA antibody used for WB at 0.04 ug/ml(1:25,000). Detection: Chemiluminescence with anexposure time of 3 seconds.")
![Western Blot: HA Tag Antibody [NB600-363] - Wnt/beta-catenin signaling regulates HySp5 expression. Immunoprecipitation (IP) of HA-tagged HySp5-420 expressed in HEK293T cells together or not with hudeltabeta-Catenin (upper) or huTCF1 (lower). IP was performed with an anti-HA antibody and Co-IP products were detected with the anti-beta-catenin or anti-TCF1 antibodies. Same results were obtained in two independent experiments. Each data point represents one biological independent experiment. Statistical p values: *p <= 0.05, **p <= 0.01, ***p <= 0.001, ****p <= 0.0001 (unpaired t test). Error bars indicate SD. PP primer pair, RLA relative luciferase activity Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-08242-2), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Western-Blot-NB600-363-img0018.jpg "Western Blot: HA Tag Antibody [NB600-363] - Wnt/beta-catenin signaling regulates HySp5 expression. Immunoprecipitation (IP) of HA-tagged HySp5-420 expressed in HEK293T cells together or not with hudeltabeta-Catenin (upper) or huTCF1 (lower). IP was performed with an anti-HA antibody and Co-IP products were detected with the anti-beta-catenin or anti-TCF1 antibodies. Same results were obtained in two independent experiments. Each data point represents one biological independent experiment. Statistical p values: *p <= 0.05, **p <= 0.01, ***p <= 0.001, ****p <= 0.0001 (unpaired t test). Error bars indicate SD. PP primer pair, RLA relative luciferase activity Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-08242-2), licensed under a CC-BY license.")
![Western Blot: HA Tag Antibody [NB600-363] - ZFN expression levels. After co-transfection of HEK293T cells with ZFN expression vectors and pEGFP, cell lysates were probed with antibodies against the HA tag and EGFP. Amount of transfected ZFN plasmids was 75 ng, 300 ng, and 1200 ng. NT, non-transfected cells. Selection-independent generation of gene knockout mouse embryonic stem cells using zinc-finger nucleases. PLoS One (2011)](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Western-Blot-NB600-363-img0017.jpg "Western Blot: HA Tag Antibody [NB600-363] - ZFN expression levels. After co-transfection of HEK293T cells with ZFN expression vectors and pEGFP, cell lysates were probed with antibodies against the HA tag and EGFP. Amount of transfected ZFN plasmids was 75 ng, 300 ng, and 1200 ng. NT, non-transfected cells. Selection-independent generation of gene knockout mouse embryonic stem cells using zinc-finger nucleases. PLoS One (2011)")
![Immunocytochemistry/Immunofluorescence: HA Tag Antibody [NB600-363] - HA-tagged proteins were detected in immersion fixed HEK293 human embryonic kidney cell line transfected with HA-tagged MGAT2 using 1 ug/mL goat anti-HA Tag polyclonal (NB600-362, Novus Biologicals), 1 ug/mL rabbit anti-HA Tag polyclonal (NB600-363, Novus Biologicals). Cells were stained using the appropriate secondary antibody, donkey anti-mouse IgG-NL557 (NL007) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Immunocytochemistry-Immunofluorescence-NB600-363-img0013.jpg "Immunocytochemistry/Immunofluorescence: HA Tag Antibody [NB600-363] - HA-tagged proteins were detected in immersion fixed HEK293 human embryonic kidney cell line transfected with HA-tagged MGAT2 using 1 ug/mL goat anti-HA Tag polyclonal (NB600-362, Novus Biologicals), 1 ug/mL rabbit anti-HA Tag polyclonal (NB600-363, Novus Biologicals). Cells were stained using the appropriate secondary antibody, donkey anti-mouse IgG-NL557 (NL007) and counterstained with DAPI (blue).")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
