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Recombinant Human GSTM3 His Protein

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Recombinant Human GSTM3 Protein [NBP1-99061]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE, Enzyme Activity
Concentration
1 mg/ml

Order Details

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Recombinant Human GSTM3 His Protein Summary

Description
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-225 of Human GSTM3

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMSCESS MVLGYWDIRG LAHAIRLLLE FTDTSYEEKR YTCGEAPDYD RSQWLDVKFK LDLDFPNLPY LLDGKNKITQ SNAILRYIAR KHNMCGETEE EKIRVDIIEN QVMDFRTQLI RLCYSSDHEK LKPQYLEELP GQLKQFSMFL GKFSWFAGEK LTFVDFLTYD ILDQNRIFDP KCLDEFPNLK AFMCRFEALE KIAAYLQSDQ FCKMPINNKM AQWGNKPVC

Details of Functionality
Specific activity is > 15,000 pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
GSTM3
Purity
>95%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • Enzyme Activity
  • SDS-Page
Theoretical MW
29.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 10% glycerol, 0.1 M NaCl
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95%, by SDS-PAGE

Alternate Names for Recombinant Human GSTM3 His Protein

  • glutathione S-transferase mu 3 (brain)
  • GST5Mu-3
  • MGC3310
  • MGC3704
  • S-(hydroxyalkyl)glutathione lyase M3

Background

Glutathione S-transferase mu 3, also known as GSTM3, is member of the glutathione s-transferase (GST) family of proteins. There are eight families of GST proteins, namely alpha, kappa, mu, omega, pi, sigma, theta and zeta, each of which is composed of proteins that have a variety of functions throughout the cell. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. Recombinant human GSTM3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol GSTM3