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Fish Growth Hormone ELISA Kit (Colorimetric)

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Fish Growth Hormone ELISA Kit (Colorimetric) [NBP3-21153] - Standard Curve Reference

Product Details

Summary
Reactivity FiSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and tissue homogenates
Standard Curve Range
312.5 pg/mL - 5000 pg/mL
Sensitivity
312.5 pg/mL

Order Details

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Fish Growth Hormone ELISA Kit (Colorimetric) Summary

Description
Assay Length: 1-5 hours
Specificity
This assay has high sensitivity and excellent specificity for detection of fish Growth Hormone. No significant cross-reactivity or interference between fish Growth Hormone and analogues was observed.
Standard Curve Range
312.5 pg/mL - 5000 pg/mL
Sensitivity
312.5 pg/mL
Assay Type
Competitive ELISA
Inter-Assay
%CV < 15
Intra-Assay
%CV < 15
Spike Recovery
90 - 98%
Sample Volume
50 - 100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
GH1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Strip (For 96 wells)
  2. Assay plate
  3. Conjugate
  4. HRP-avidin
  5. Instruction manual
  6. Standard
  7. Stop Solution
  8. Substrate A
  9. Substrate B
  10. Wash Buffer (20 x concentrate)

Alternate Names for Fish Growth Hormone ELISA Kit (Colorimetric)

  • GH1
  • GHB5
  • GHIGHD1B
  • GHN
  • GH-N
  • GHNGrowth hormone
  • GH-Nsomatotropin
  • growth hormone 1Pituitary growth hormone
  • Growth Hormone
  • hGH-N
  • IGHD1A
  • IGHD2
  • Somatotropin

Background

Human growth hormone (hGH) plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. The effects of hGH in the body can be generally described as anabolic. It stimulates amino acid uptake and protein synthesis in muscle and other tissues. Growth Hormone (GH1) is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Genes for growth hormone (GH) are found in a gene cluster on 17q, which consists of two growth hormone genes and three CSH genes. The GHN1 gene is transcribed exclusively in the pituitary, whereas the other 4 genes are expressed only in placental tissues. Several isoforms of GH1 exist. Human growth hormone (hGH) is also used as a reporter protein, because it is secreted from mammalian cells it can be assayed by ELISA on culture supernatant using antibodies to determine the level of expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Additional Growth Hormone Products

Research Areas for Growth Hormone ELISA Kit (NBP3-21153)

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Blogs on Growth Hormone.

Growth hormone (GH, somatotropin, hGH, pituitary growth hormone)
GH is a member of the large family of growth factors that includes prolactin, placental lactogens, proliferins, and somatolactin. Additionally, GH is a 191-amino acid, single-chain polypeptide that is synthesized, stored, and secreted by somatotropic ...  Read full blog post.

Somatostatin Receptor 2: Treating Patients Who Cannot Stop Growing
Acromegaly is a rare life-shortening disease caused by elevated levels of growth hormone (GH) secreted by a tumor on the pituitary gland. Treatments include somatostatin analogs, which activate somatostatin receptor 2 (SSTR2), reducing GH secretion an...  Read full blog post.

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Bioinformatics

Gene Symbol GH1