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Growth Hormone Antibody

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Growth Hormone was detected in perfusion fixed frozen sections of rat pituitary using Goat Anti-Rat Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1566) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Growth Hormone was detected in perfusion fixed frozen sections of rat pituitary using Goat Anti-Rat Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1566) at 15 µg/mL overnight at 4 °C. Tissue ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF1566
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Catalog# & Formulation Size Price
Carrier Free
AF1566-SP
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Growth Hormone Antibody Summary

Immunogen
E. coli-derived recombinant rat Growth Hormone
Leu27-Phe216
Accession # P01244
Specificity
Detects rat Growth Hormone in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Growth Hormone is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GH1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF1566 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Growth Hormone Antibody

  • GH1
  • GHB5
  • GHIGHD1B
  • GHN
  • GH-N
  • GHNGrowth hormone
  • GH-Nsomatotropin
  • growth hormone 1Pituitary growth hormone
  • Growth Hormone
  • hGH-N
  • IGHD1A
  • IGHD2
  • Somatotropin

Background

Growth Hormone, also named somatotropin, belongs to the somatotropin/prolactin superfamily. It is synthesized in the anterior pituitary gland. Growth Hormone regulates body growth by stimulating the secretion of IGF-I by liver and other tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Growth Hormone Antibody (AF1566)(9)

We have publications tested in 4 confirmed species: Mouse, Rat, Gecko, Porcine.

We have publications tested in 4 applications: IF, IHC, IHC-P, Western Blot.


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(1)
IHC
(1)
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(1)
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(2)
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Showing Publications 1 - 9 of 9.
Publications using AF1566 Applications Species
Jinah Ha, Jungwoo Shin, Eunji Seok, Soohyun Kim, Sojung Sun, Hyunwon Yang Estradiol and progesterone regulate NUCB2/nesfatin-1 expression and function in GH3 pituitary cells and THESC endometrial cells Animal Cells and Systems 2023-12-11 [PMID: 37351263]
Debdeep Chatterjee, Reeba S Jacob, Soumik Ray, Ambuja Navalkar, Namrata Singh, Shinjinee Sengupta et al. Co-aggregation and secondary nucleation in the life cycle of human prolactin/galanin functional amyloids eLife 2022-03-08 [PMID: 35257659]
I Hofmann, E Kemter, S Fiedler, N Theobalt, L Fonteyne, E Wolf, R Wanke, A Blutke A new method for physical disector analyses of numbers and mean volumes of immunohistochemically labelled cells in paraffin sections Journal of neuroscience methods, 2021-07-01;361(0):109272. 2021-07-01 [PMID: 34216707] (IHC, Porcine) IHC Porcine
M Yang, X Lin, F Segers, R Suganthan, GA Hildrestra, JE Rinholm, PA Aas, MML Sousa, S Holm, N Bolstad, D Warren, RK Berge, RF Johansen, A Yndestad, E Kristianse, A Klungland, L Luna, L Eide, B Halvorsen, P Aukrust, M Bjørås OXR1A, a Coactivator of PRMT5 Regulating Histone Arginine Methylation Cell Rep, 2020-03-24;30(12):4165-4178.e7. 2020-03-24 [PMID: 32209476] (Western Blot, Mouse) Western Blot Mouse
H Suzuki, T Yamamoto Chemokine CXCL14-like immunoreactivity in the &alphaMSH-producing cells and PRL-producing cells of the flat-tailed house gecko pituitary J. Vet. Med. Sci., 2020-02-10;82(4):408-413. 2020-02-10 [PMID: 32037367] (IF, Gecko) IF Gecko
Arne Hinrichs, Barbara Kessler, Mayuko Kurome, Andreas Blutke, Elisabeth Kemter, Maren Bernau et al. Growth hormone receptor-deficient pigs resemble the pathophysiology of human Laron syndrome and reveal altered activation of signaling cascades in the liver Molecular Metabolism 2018-05-01 [PMID: 29678421]
Takehiro Tsukada, Ken Fujiwara, Kotaro Horiguchi, Morio Azuma, Dini Ramadhani, Alimuddin Tofrizal et al. Folliculostellate Cells Are Required for Laminin Release from Gonadotrophs in Rat Anterior Pituitary ACTA HISTOCHEMICA ET CYTOCHEMICA 2014-10-23 [PMID: 25861130]
Giles A, Madec F, Friedrichsen S, Featherstone K, Chambers T, Harper CV, Resch J, Brabant G, Davis JR Wnt signaling in estrogen-induced lactotroph proliferation. J. Cell. Sci., 2011-01-18;124(0):540-7. 2011-01-18 [PMID: 21245194] (IHC-P, Rat) IHC-P Rat
Kasturi BS, Stein DG Traumatic brain injury causes long-term reduction in serum growth hormone and persistent astrocytosis in the cortico-hypothalamo-pituitary axis of adult male rats. J. Neurotrauma, 2009-08-01;26(8):1315-24. 2009-08-01 [PMID: 19317601] (Western Blot, Rat) Western Blot Rat

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Secondary Antibodies

 

Isotype Controls

Additional Growth Hormone Products

Blogs on Growth Hormone.

Check out the latest blog posts on Growth Hormone.
Growth hormone (GH, somatotropin, hGH, pituitary growth hormone)
GH is a member of the large family of growth factors that includes prolactin, placental lactogens, proliferins, and somatolactin. Additionally, GH is a 191-amino acid, single-chain polypeptide that is synthesized, stored, and secreted by somatotropic ...  Read full blog post.
Somatostatin Receptor 2: Treating Patients Who Cannot Stop Growing
Acromegaly is a rare life-shortening disease caused by elevated levels of growth hormone (GH) secreted by a tumor on the pituitary gland. Treatments include somatostatin analogs, which activate somatostatin receptor 2 (SSTR2), reducing GH secretion an...  Read full blog post.
Read our latest blog and use the new citation tool on bio-techne.com

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Bioinformatics

Gene Symbol GH1
Uniprot