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Glucagon Quantikine ELISA Kit

Product Details

Summary
Reactivity All-MultiSpecies Glossary
Applications ELISA
Conjugate
HRP

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Glucagon Quantikine ELISA Kit Summary

Background
The Quantikine Glucagon Immunoassay is a 4.5 hour solid-phase ELISA designed to measure Glucagon in cell culture supernates, serum, and plasma. It contains natural porcine Glucagon as the standard. The antibodies were raised against a human Glucagon synthetic peptide. This immunoassay has been shown to accurately quantitate human, mouse, rat, and porcine Glucagon.
Specificity
Natural Glucagon.  This assay cross-reacts <12% with Oxyntomodulin.
Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DGCG0.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glucagon Quantikine ELISA Kit

  • GCG
  • glicentin-related polypeptide
  • GLP1
  • GLP-1
  • Glucagon
  • glucagon-like peptide 1
  • glucagon-like peptide 2
  • GRPP

Background

Glucagon is a 29 amino acid (aa) peptide produced by the pancreas that plays a critical role in glucose metabolism and homeostasis (1-4). The Glucagon precursor mRNA is expressed by alpha cells ( alpha -cells) of the pancreas, L cells of the intestine, and in the brain (1, 2). Only the pancreatic alpha -cells express the prohormone convertase PC2, also called PCSK2, which is required to produce Glucagon (2). Intestinal L cells instead express the prohormone convertase PC1, which processes the precursor to the Glucagon-overlapping peptides glicentin and oxyntomodulin. L cells also produce two Glucagon-like peptides, GLP-1 and GLP-2 that are derived from the same Glucagon precursor and influence glucose metabolism, but do not share any common sequence with Glucagon (1, 2). The aa sequence of the mature Glucagon peptide is identical in human, mouse, rat, pig, dog, horse, cow, sheep, and Xenopus. 
In normal metabolism, Glucagon is secreted in response to low blood glucose (hypoglycemia) and downregulated in response to high blood glucose (hyperglycemia). Although Glucagon binding sites are found in liver, brain, pancreas, kidney, intestine, and adipose tissue, the main activity of Glucagon receptors occurs in the liver, where Glucagon stimulates gluconeogenesis and glycogenolysis, thereby increasing blood glucose (1-4). It is particularly important that the brain receive sufficient glucose, since it is unable to store more than a minute quantity. Therefore the release of Glucagon from alpha -cells is under control by both hormones and neurotransmitters, and is very responsive to circulating glucose concentration. Insulin, and/or the zinc that islet beta cells secrete with it, downregulates Glucagon secretion in intact islets (5, 6). Glucagon secretion is also downregulated by the neurotransmitter gamma -aminobutyric acid (GABA), somatostatin produced by islet δ-cells, and GLP-1, but is enhanced by the neurotransmitter L-glutamate, amino acids (especially arginine), and Glucagon itself (2-4, 7). Through receptors on the alpha -cells, these substances affect potassium, sodium, and calcium channel activity and alter intracellular calcium concentration (2-4). Glucose suppression of Glucagon secretion is probably indirect, acting through paracrine signals from other islet cells (8). 
Like insulin, Glucagon is dysregulated in type 2 diabetes (T2D) and contributes to its pathology (2-4). Glucagon secretion is less responsive to insulin-mediated suppression in times of high circulating glucose, causing glucagonemia, and increasing the risk of hyperglycemia. Glucagon is also regulated by some of the same messengers that regulate insulin (10-12). Leptin inhibits alpha -cell glucagon secretion and stimulates beta -cell insulin secretion, but glucagon blunts the leptin-mediated insulin secretion (10). Islet alpha -cells express ghrelin receptors and respond to ghrelin by increasing Glucagon secretion (11). Glucocorticoids, activated by 11 beta -HSD1, depress Glucagon secretion in hypoglycemia and insulin secretion in hyperglycemia (12). Although genetic polymorphisms of the Glucagon receptor are associated with T2D, downregulation of Glucagon secretion or deletion of the Glucagon receptor in mice that are susceptible to T2D actually improves glycemic control (13, 14).

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&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Glucagon (DGCG0)(17)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.


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Human
(6)
Mouse
(9)
Rat
(3)
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Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using DGCG0 Applications Species
Evans-Molina, C;Pettway, YD;Saunders, DC;Sharp, SA;Bate, TS;Sun, H;Durai, H;Mei, S;Coldren, A;Davis, C;Reihsmann, CV;Hopkirk, AL;Taylor, J;Bradley, A;Aramandla, R;Poffenberger, G;Eskaros, A;Jenkins, R;Shi, D;Kang, H;Rajesh, V;Thaman, S;Feng, F;Cartailler, JP;Powers, AC;Abraham, K;Gloyn, AL;Niland, JC;Brissova, M; Heterogeneous endocrine cell composition defines human islet functional phenotypes bioRxiv : the preprint server for biology 2024-11-21 [PMID: 39605606] (Human) Human
Shin, SK;Kwon, EY; Kaempferol ameliorates metabolic syndrome by inhibiting inflammation and oxidative stress in high-fat diet-induced obese mice Nutrition research and practice 2024-06-01 [PMID: 38854471] (Mouse) Mouse
Li, Y;Zhou, X;Cheng, C;Ding, G;Zhao, P;Tan, K;Chen, L;Perrimon, N;Veenstra, JA;Zhang, L;Song, W; Gut AstA mediates sleep deprivation-induced energy wasting in Drosophila Cell discovery 2023-05-23 [PMID: 37221172] (Mouse) Mouse
EC Lien, AM Westermark, Y Zhang, C Yuan, Z Li, AN Lau, KM Sapp, BM Wolpin, MG Vander Hei Low glycaemic diets alter lipid metabolism to influence tumour growth Nature, 2021-10-20;0(0):. 2021-10-20 [PMID: 34671163] (Human) Human
Y Bai, K Mo, G Wang, W Chen, W Zhang, Y Guo, Z Sun Intervention of Gastrodin in Type 2 Diabetes Mellitus and Its Mechanism Frontiers in Pharmacology, 2021-09-16;12(0):710722. 2021-09-16 [PMID: 34603025] (Rat) Rat
S Lang, R Wei, T Wei, L Gu, J Feng, H Yan, J Yang, T Hong Glucagon receptor antagonism promotes the production of gut proglucagon-derived peptides in diabetic mice Peptides, 2020-06-16;131(0):170349. 2020-06-16 [PMID: 32561493] (Mouse) Mouse
CC Hsiao, CC Lin, YS Hou, JY Ko, CJ Wang Low-Energy Extracorporeal Shock Wave Ameliorates Streptozotocin Induced Diabetes and Promotes Pancreatic Beta Cells Regeneration in a Rat Model Int J Mol Sci, 2019-10-05;20(19):. 2019-10-05 [PMID: 31590394] (Rat) Rat
D Zhou, YW Chen, ZH Zhao, RX Yang, FZ Xin, XL Liu, Q Pan, H Zhou, JG Fan Sodium butyrate reduces high-fat diet-induced non-alcoholic steatohepatitis through upregulation of hepatic GLP-1R expression Exp. Mol. Med., 2018-12-03;50(12):157. 2018-12-03 [PMID: 30510243] (Human) Human
I Roncero-Ra, R Jimenez-Lu, JF Alcala-Dia, C Vals-Delga, AP Arenas-Lar, OA Rangel-Zuñ, A Leon-Acuña, MM Malagon, J Delgado-Li, P Perez-Mart, JM Ordovas, A Camargo, J Lopez-Mira Alpha cell function interacts with diet to modulate prediabetes and Type 2 diabetes J. Nutr. Biochem., 2018-09-01;62(0):247-256. 2018-09-01 [PMID: 30336335] (Human) Human
V Sancho, G Daniele, D Lucchesi, R Lupi, A Ciccarone, G Penno, C Bianchi, A Dardano, R Miccoli, S Del Prato Metabolic regulation of GLP-1 and PC1/3 in pancreatic ?-cell line PLoS ONE, 2017-11-09;12(11):e0187836. 2017-11-09 [PMID: 29121068] (Mouse) Mouse
Show All 17 Publications.

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