![Western Blot: Glu-Glu Epitope Tag Antibody [NB600-354] - Analysis using the HRP conjugate of NB600-354. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.2 ug/ml (1:5,000).](http://images.novusbio.com/fullsize/Glu-Glu-Epitope-Tag-Antibody-Western-Blot-NB600-354-img0003.jpg "Western Blot: Glu-Glu Epitope Tag Antibody [NB600-354] - Analysis using the HRP conjugate of NB600-354. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.2 ug/ml (1:5,000).")
| Reactivity | ETSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | Rabbits were immunized with glu-glu cleavage site (EYMPME) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support. Rabbit anti- human glu-glu affinity purified antibodies were coupled to peptide immobilized on solid support. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Suggested working dilutions: * ELISA Coating 1:100-1:500Primary 1:1000-1:30,000 Western Blots Colorimetric detection 1:1000-1:10,000ECL 1:1000-1:30,000 Cyto 1:100-1:400 *The investigator should determine the optimal working dilution for a specific application. ELISA, Western blot and immunocytochemistry. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Kalynn Alexander |
Flow | Mouse | 08/24/2023 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
Research Areas for Glu-Glu Epitope Tag Antibody (NB600-354)Find related products by research area.
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![Western Blot: Glu-Glu Epitope Tag Antibody [NB600-354] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).](http://images.novusbio.com/fullsize/Glu-Glu-Epitope-Tag-Antibody-Western-Blot-NB600-354-img0002.jpg "Western Blot: Glu-Glu Epitope Tag Antibody [NB600-354] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).")
![Western Blot: Glu-Glu Epitope Tag Antibody [NB600-354] - Triton X-100(1%0 Whole cell lysate(20mcg) from Cos7 cells transiently transfected with control vector (v) or an expression vector driving expression of Glu-Glu tagged RalBP1 (a to d).](http://images.novusbio.com/fullsize/Glu-Glu-Epitope-Tag-Antibody-Western-Blot-NB600-354-img0001.jpg "Western Blot: Glu-Glu Epitope Tag Antibody [NB600-354] - Triton X-100(1%0 Whole cell lysate(20mcg) from Cos7 cells transiently transfected with control vector (v) or an expression vector driving expression of Glu-Glu tagged RalBP1 (a to d).")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
