| Leonid Andronov, Mengting Han, Yanyu Zhu, Ashwin Balaji, Anish R Roy, Andrew E S Barentine, Puja Patel, Jaishree Garhyan, Lei S Qi, W E Moerner Nanoscale cellular organization of viral RNA and proteins in SARS-CoV-2 replication organelles. Nature communications 2024-05-31 [PMID: 38821943] |
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| Yifan Wang, L. Robert Hollingsworth, Lamba Omar Sangaré, Tatiana C. Paredes-Santos, Shruthi Krishnamurthy, Bennett H. Penn, Hao Wu, Jeroen P. J. Saeij, Louis M. Weiss Host E3 ubiquitin ligase ITCH mediates Toxoplasma gondii effector GRA35-triggered NLRP1 inflammasome activation and cell-autonomous immunity mBio 2024-03-01 [PMID: 38376248] |
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ICC/IF |
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| Hirano T, Ebine K, Ueda T et al. The SYP123-VAMP727 SNARE complex delivers secondary cell wall components for root hair shank hardening in Arabidopsis The Plant cell 2023-09-15 [PMID: 37713604] (WB) |
WB |
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| Callado Pérez A, Demers M, Fassihi A et al. A brainstem circuit for the expression of defensive facial reactions in rat Current biology : CB 2023-09-25 [PMID: 37703878] (IHC) |
IHC |
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| Toth BA, Chang KS, Fechtali S, Burgess CR. Dopamine release in the nucleus accumbens promotes REM sleep and cataplexy iScience 2023-09-15 [PMID: 37664637] (Immunohistochemistry) |
Immunohistochemistry |
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| Ansell TB, Corey RA, Viti LV et al. The energetics and ion coupling of cholesterol transport through Patched1 Science Advances 2023-08-25 [PMID: 37611095] (Western Blot) |
Western Blot |
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| Jie Yang, Kelsey R. Baron, Daniel E. Pride, Anette Schneemann, Xiaoyan Guo, Wenqian Chen, Albert S. Song, Giovanni Aviles, Martin Kampmann, R. Luke Wiseman, Gabriel C. Lander DELE1 oligomerization promotes integrated stress response activation Nature structural & molecular biology 2023-09-27 [PMID: 37550454] |
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| Krauchunas AR, Marcello MR, Looper A et al. The EGF-motif-containing protein SPE-36 is a secreted sperm protein required for fertilization in C. elegans Current biology : CB 2023-07-24 [PMID: 37453426] (WB) |
WB |
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Thagun C, Suzuki T, Kodama Y, Numata K C-Terminal Domain Controls Protein Quality and Secretion of Spider Silk in Tobacco Cells Advanced biology 2023-07-06 [PMID: 37409415] (IB)
Details:
N. tabacum |
IB |
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| Toth BA, Chang KS, Burgess CR. Striatal dopamine regulates sleep states and narcolepsy-cataplexy bioRxiv 2023-06-03 [PMID: 37397994] (Immunohistochemistry) |
Immunohistochemistry |
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Xing L, Simon JM, Ptacek TS et al. Autism-linked UBE3A gain-of-function mutation causes interneuron and behavioral phenotypes when inherited maternally or paternally in mice Cell reports 2023-06-28 [PMID: 37389991] (WB)
Details:
WB 1:2000 |
WB |
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Samuel T, Rapic S, O'Brien C et al. Quantitative intravital imaging for real-time monitoring of pancreatic tumor cell hypoxia and stroma in an orthotopic mouse model Science advances 2023-06-09 [PMID: 37285434] (WB)
Details:
1:50 WB dilution |
WB |
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| Bao K, Isik Can U, Miller MM et al. A bifurcated role for c-Maf in Th2 and Tfh2 cells during helminth infection Mucosal immunology 2023-04-21 [PMID: 37088263] (IHC) |
IHC |
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| Xing D, Hage FG, Feng W et al. Endothelial Cells Overexpressing CXCR1/2 are Renoprotective in Rats with Acute Kidney Injury American journal of physiology. Renal physiology 2023-02-16 [PMID: 36794755] (IHC-P, Rat) |
IHC-P |
Rat |
| Li Q, Kaur A, Okada K et al. Differential requirement for BRCA1-BARD1 E3 ubiquitin ligase activity in DNA damage repair and meiosis in the Caenorhabditis elegans germ line PLOS Genetics 2023-01-30 [PMID: 36716349] (Western Blot) |
Western Blot |
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| Sanchita Rauth, Koelina Ganguly, Pranita Atri, Seema Parte, Rama Krishna Nimmakayala, Venkatesh Varadharaj, Palanisamy Nallasamy, Raghupathy Vengoji, Ayoola O Ogunleye, Imayavaramban Lakshmanan, Ramakanth Chirravuri, Mika Bessho, Jesse L Cox, Jason M Foster, Geoffrey A Talmon, Tadayoshi Bessho, Apar Kishor Ganti, Surinder K Batra, Moorthy P Ponnusamy Elevated PAF1-RAD52 axis confers chemoresistance to human cancers. Cell reports 2023-01-28 [PMID: 36709426] |
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| Yuka Nago-Iwashita, Yuki Moriya, Satoshi Hara, Ryohei Ogawa, Rina Aida, Katsuya Miyajima, Takenobu Shimura, Shin-Ichi Muramatsu, Soichiro Ide, Kazutaka Ikeda, Hiroshi Ichinose Overexpression of tyrosine hydroxylase in dopaminergic neurons increased sensitivity to methamphetamine. Neurochemistry international 2023-03-14 [PMID: 36709046] |
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| Boffa I, Polishchuk E, De Stefano L et al. Liver-directed gene therapy for ornithine aminotransferase deficiency EMBO molecular medicine 2023-04-11 [PMID: 36647689] (Western Blot) |
Western Blot |
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Obara K, Shirai K, Hamada Y et al. Direct implantation of hair-follicle-associated pluripotent (HAP) stem cells repairs intracerebral hemorrhage and reduces neuroinflammation in mouse model PloS one 2023-01-13 [PMID: 36638123] (IHC-P, Mouse)
Details:
Dilution used in IHC-P 1:1000 |
IHC-P |
Mouse |
Stupin A Atg1 modulates mitochondrial dynamics to promote germline stem cell maintenance in Drosophila Biochemical and Biophysical Research Communications 2022-11-01 [PMID: 36621115] (IF/IHC)
Details:
Drosophila expressing GFP |
IF/IHC |
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| Vrathasha V, Nikonov S, Bell B et al. Transplanted human induced pluripotent stem cells- derived retinal ganglion cells embed within mouse retinas and are electrophysiologically functional iScience 2022-10-01 [PMID: 36388952] |
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| Yamada S, Mizukoshi T, Tokunaga A, Sakakibara SI. Inka2, a novel Pak4 inhibitor, regulates actin dynamics in neuronal development PLOS Genetics 2022-10-27 [PMID: 36301793] (Immunoprecipitation, Immunocytochemistry/ Immunofluorescence) |
Immunoprecipitation, Immunocytochemistry/ Immunofluorescence |
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| McNally KP, Beath EA, Danlasky BM et al. Cohesin is required for meiotic spindle assembly independent of its role in cohesion in C. elegans PLOS Genetics 2022-10-24 [PMID: 36279281] (Immunocytochemistry/ Immunofluorescence) |
Immunocytochemistry/ Immunofluorescence |
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| Le-Trilling VTK, Ebel JF, Baier F et al. Acute cytomegalovirus infection modulates the intestinal microbiota and targets intestinal epithelial cells European journal of immunology 2023-02-01 [PMID: 36250419] (Immunohistochemistry) |
Immunohistochemistry |
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| Seo-Yeon Jeong, Gurusamy Hariharasudhan, Min-Ji Kim, Ji-Yeon Lim, Sung Mi Jung, Eun-Ji Choi, In-Youb Chang, Younghoon Kee, Ho Jin You, Jung-Hee Lee SIAH2 regulates DNA end resection and replication fork recovery by promoting CtIP ubiquitination Nucleic Acids Research 2022-10-14 [PMID: 36155803] |
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| Zhang W, Lanzoni G, Hani H et al. Patch Grafting of Cells into Solid Organs Such as Liver and Pancreas Biomaterials 2022-08-27 [PMID: 36030102] |
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Rourke C, Jaramillo-Lambert A TOP-2 is differentially required for the proper maintenance of the cohesin subunit REC-8 on meiotic chromosomes in C. elegans spermatogenesis and oogenesis Genetics 2022-08-11 [PMID: 35951744] (IHC-Fr, Human)
Details:
IHC-Fr 1:500 |
IHC-Fr |
Human |
| Nam YW, Pala R, El-Sayed NS et al. Subtype-Selective Positive Modulation of KCa2.3 Channels Increases Cilia Length ACS chemical biology 2022-08-19 [PMID: 35947779] (WB, Mouse) |
WB |
Mouse |
| Ma JH, Lee E, Yoon SH et al. Therapeutic effect of NLRP3 inhibition on hearing loss induced by systemic inflammation in a CAPS-associated mouse model EBioMedicine 2022-07-20 [PMID: 35870427] (IHC-Fr) |
IHC-Fr |
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| Hanna DN, Smith PM, Novitskiy SV et al. SMAD4 suppresses colitis-associated carcinoma through inhibition of CCL20/CCR6-mediated inflammation Gastroenterology 2022-07-18 [PMID: 35863523] |
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| Osaki Y, Manolopoulou M, Ivanova AV et al. Blocking cell cycle progression through CDK4/6 protects against chronic kidney disease JCI insight 2022-06-22 [PMID: 35730565] (FLOW, IF/IHC) |
FLOW, IF/IHC |
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| Feng C, Wang Y, Zha X et al. Cold-sensitive ventromedial hypothalamic neurons control homeostatic thermogenesis and social interaction-associated hyperthermia Cell metabolism 2022-06-07 [PMID: 35675799] (IF/IHC) |
IF/IHC |
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| Takaaki Miyamoto, Kousuke Tsuchiya, Kiminori Toyooka, Yumi Goto, Ayaka Tateishi, Keiji Numata Relaxation of the Plant Cell Wall Barrier via Zwitterionic Liquid Pretreatment for Micelle‐Complex‐Mediated DNA Delivery to Specific Plant Organelles Angewandte Chemie (International Ed. in English) 2022-06-24 [PMID: 35670289] |
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| George AJ, Dong B, Lail H et al. The E3 ubiquitin ligase RNF216/TRIAD3 is a key coordinator of the hypothalamic-pituitary-gonadal axis iScience 2022-06-17 [PMID: 35620441] (ELISA, Western Blot) |
ELISA, Western Blot |
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| Ma L, Chen C, Liu D et al. Apoptotic extracellular vesicles are metabolized regulators nurturing the skin and hair Bioactive materials 2023-01-01 [PMID: 35600968] (ICC/IF) |
ICC/IF |
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| Law SSY, Liou G, Nagai Y et al. Polymer-coated carbon nanotube hybrids with functional peptides for gene delivery into plant mitochondria Nature communications 2022-05-16 [PMID: 35577779] (WB) |
WB |
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| Kim H, Gao EB, Draper A et al. Rescue of behavioral and electrophysiological phenotypes in a Pitt-Hopkins syndrome mouse model by genetic restoration of Tcf4 expression eLife 2022-05-10 [PMID: 35535852] |
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| Gu T, Hao D, Woo J et al. The disordered N-terminal domain of DNMT3A recognizes H2AK119ub and is required for postnatal development Nature genetics 2022-05-01 [PMID: 35534561] (WB) |
WB |
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| Lao M, Hurtado A, de Castro AC et al. Sox9 is required for nail bed differentiation and digit tip regeneration The Journal of investigative dermatology 2022-04-07 [PMID: 35398376] |
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| Morales L, Gonz�lez-Alonso A, Desfilis E, Medina L. Precise Mapping of Otp Expressing Cells Across Different Pallial Regions Throughout Ontogenesis Using Otp-Specific Reporter Transgenic Mice Frontiers in Neural Circuits 2022-02-17 [PMID: 35250495] |
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| Thagun C, Horii y, Mori M et al. Non-transgenic Gene Modulation via Spray Delivery of Nucleic Acid/Peptide Complexes into Plant Nuclei and Chloroplasts ACS nano 2022-02-23 [PMID: 35195009] (WB) |
WB |
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| Sun J, Yoon J, Lee M et al. Zic5 stabilizes Gli3 via a non-transcriptional mechanism during retinal development Cell reports 2022-02-01 [PMID: 35108539] (ICC/IF) |
ICC/IF |
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| Chalif JI, MartInez-Silva ML, Pagiazitis JG et al. Control of mammalian locomotion by ventral spinocerebellar tract neurons Cell 2022-01-20 [PMID: 35063074] (IHC-P) |
IHC-P |
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| Chen B, Scurrah CR, McKinley ET et al. Differential pre-malignant programs and microenvironment chart distinct paths to malignancy in human colorectal polyps Cell 2021-12-22 [PMID: 34910928] (FLOW) |
FLOW |
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| Shu XE, Mao Y, Jia L, Qian SB Dynamic eIF3a O-GlcNAcylation controls translation reinitiation during nutrient stress Nature chemical biology 2021-12-09 [PMID: 34887587] (IP) |
IP |
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| Joshi B, Wagh G, Kaur H, Patra C Zebrafish Model to Study Angiotensin II-Mediated Pathophysiology Biology 2021-11-13 [PMID: 34827169] (IF/IHC) |
IF/IHC |
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| McGuire MR, Mukhopadhyay D, Myers SL Et al. Progesterone receptor membrane component 1 (PGRMC1) binds and stabilizes cytochromes P450 through a heme-independent mechanism The Journal of biological chemistry 2021-10-19 [PMID: 34678314] (WB) |
WB |
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| Becker R Regulation of non-centrosomal microtubule organizing center formation in skeletal muscle cells Thesis 2022-01-01 |
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IF/IHC |
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| Wu Y, Devotta A, JosE-Edwards DS et al. Xbp1 and Brachyury establish an evolutionarily conserved subcircuit of the notochord gene regulatory network eLife 2022-01-20 [PMID: 35049502] (ISH) |
ISH |
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| Ramos H, Monette A, Niu M et al. The double-stranded RNA-binding protein, Staufen1, is an IRES-transacting factor regulating HIV-1 cap-independent translation initiation Nucleic acids research 2021-12-06 [PMID: 34893869] (WB) |
WB |
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| Rahman F, Johnson JL, Zhang J et al. DYNC1LI2 regulates localization of the chaperone-mediated autophagy receptor LAMP2A and improves cellular homeostasis in cystinosis Autophagy 2022-05-04 [PMID: 34643468] |
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Hausmann A, Felmy B, Kunz L et al. Intercrypt sentinel macrophages tune antibacterial NF-kappa B responses in gut epithelial cells via TNF J Exp Med 2021-11-01 [PMID: 34529751] (ICC/IF)
Details:
Citation using the Alexa Fluor 647 format of this antibody. |
ICC/IF |
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| Delpech JC, Pathak D, Varghese M et al. Wolframin-1-expressing neurons in the entorhinal cortex propagate tau to CA1 neurons and impair hippocampal memory in mice Science Translational Medicine 2021-09-15 [PMID: 34524859] (Electron Microscopy) |
Electron Microscopy |
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| Zhang W, Lanzoni G, Hani H Et al. Patch grafting, strategies for transplantation of organoids into solid organs such as liver Biomaterials 2021-08-01 [PMID: 34517276] (ICC/IF) |
ICC/IF |
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| Kong JH, Young CB, Pusapati GV Et al. Gene-teratogen interactions influence the penetrance of birth defects by altering Hedgehog signaling strength Development (Cambridge, England) 2021-10-01 [PMID: 34486668] |
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| Huang YH, Chen CW, Sundaramurthy V et al. Systematic Profiling of DNMT3A Variants Reveals Protein Instability Mediated by the DCAF8 E3 Ubiquitin Ligase Adaptor Cancer Discovery 2022-01-01 [PMID: 34429321] (Western Blot) |
Western Blot |
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| Autry AE, Wu Z, Kohl J, Bambah-Mukku D Perifornical Area Urocortin-3 Neurons Promote Infant-directed Neglect and Aggression Elife 2021-08-23 [PMID: 34423776] |
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| Girish N, Liu CY, Gadeock S et al. Persistence of Lgr5+ colonic epithelial stem cells in mouse models of inflammatory bowel disease American journal of physiology. Gastrointestinal and liver physiology 2021-07-14 [PMID: 34260310] (IHC-P) |
IHC-P |
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| Bourgeois Js, Wang L, Rabino Af Et Al. ARHGEF26 enhances Salmonella invasion and inflammation in cells and mice PLoS pathogens 2021-07-01 [PMID: 34242364] (Cytometric Bead Assay Standard) |
Cytometric Bead Assay Standard |
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| Singh B, Khattab F, Chae H et al. KATP channel blockers control glucagon secretion by distinct mechanisms: The direct stimulation of alpha-cells involving a [Ca2+]c rise and an indirect inhibition mediated by somatostatin Molecular metabolism 2021-06-09 [PMID: 34118477] (IF/IHC) |
IF/IHC |
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| Xu PF, Borges RM, Fillatre J et al. Construction of a mammalian embryo model from stem cells organized by a morphogen signalling centre Nature communications 2021-06-02 [PMID: 34078907] (IF/IHC) |
IF/IHC |
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| Constantine Mylonas, Choongman Lee, Alexander L Auld, Ibrahim I Cisse, Laurie A Boyer A dual role for H2A.Z.1 in modulating the dynamics of RNA polymerase II initiation and elongation. Nature structural & molecular biology 2021-07-21 [PMID: 33972784] |
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| McElvain LE, Chen Y, Moore JD et al. Specific populations of basal ganglia output neurons target distinct brain stem areas while collateralizing throughout the diencephalon Neuron 2021-04-01 [PMID: 33823137] |
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| Maniates KA, Olson BS, Abbott AL Sperm fate is promoted by the mir-44 microRNA family in the Caenorhabditis elegans hermaphrodite germline Genetics 2021-03-08 [PMID: 33683352] |
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| Yu D, G Febbo I, J Maroteaux M et al. The Transcription Factor Shox2 Shapes Neuron Firing Properties and Suppresses Seizures by Regulation of Key Ion Channels in Thalamocortical Neurons Cerebral cortex (New York, N.Y. : 1991) 2021-03-02 [PMID: 33675359] (IF/IHC, WB) |
IF/IHC, WB |
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| Oikawa K, Imai T, Thagun C et al. Mitochondrial movement during its association with chloroplasts in Arabidopsis thaliana Communications biology 2021-03-05 [PMID: 33674706] (WB) |
WB |
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| Tang C, Han J, Dalvi S et al. A human model of Batten disease shows role of CLN3 in phagocytosis at the photoreceptor-RPE interface Commun Biol 2021-02-05 [PMID: 33547385] (WB) |
WB |
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| González-Prieto R, Eifler-Olivi K, Claessens LA, et al. Global non-covalent SUMO interaction networks reveal SUMO-dependent stabilization of the non-homologous end joining complex Cell reports 2021-01-26 [PMID: 33503430] |
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| Morales L, Castro-Robles B, AbellAn A et al. A novel telencephalon-opto-hypothalamic morphogenetic domain produces most of the glutamatergic neurons of the medial extended amygdala J Comp Neurol 2021-01-02 [PMID: 33386618] |
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| Vergarajauregui S, Becker R, Steffen U et al. AKAP6 orchestrates the nuclear envelope microtubule-organizing center by linking golgi and nucleus via AKAP9 eLife 2020-12-09 [PMID: 33295871] (Non-species specific) |
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Non-species specific |
| Bin Yu, Shoeb Ikhlas, Chunsheng Ruan, Xingxing Zhong, Dongsheng Cai Innate and Adaptive Immunity of Murine Neural Stem Cell-Derived piRNA Exosomes/Microvesicles against Pseudotyped SARS-CoV-2 and HIV-Based Lentivirus iScience 2020-11-13 [PMID: 33205008] |
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| Wolter JM, Mao H, Fragola G et al. Cas9 gene therapy for Angelman syndrome traps Ube3a-ATS long non-coding RNA Nature 2020-10-21 [PMID: 33087932] |
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| Sade N, Weng F, Tajima H et al. A Cytoplasmic Receptor-like Kinase Contributes to Salinity Tolerance Plants (Basel) 2020-10-17 [PMID: 33080797] |
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| Im JY, Kim DM, Park H et al. Bacterial Lipoteichoic Acid Attenuates Toll-Like Receptor Dependent Dendritic Cells Activation and Inflammatory Response Biochim Biophys Acta Mol Cell Res 2020-10-08 [PMID: 33069758] (WB) |
WB |
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| Danlasky BM, Panzica MT, McNally KP et al. Evidence for anaphase pulling forces during C. elegans meiosis Journal of Cell Biology 2020-12-07 [PMID: 33064834] (Immunocytochemistry/ Immunofluorescence) |
Immunocytochemistry/ Immunofluorescence |
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IF/IHC |
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| Kong JH, Young CB, Pusapati GV et al. A Membrane-Tethered Ubiquitination Pathway Regulates Hedgehog Signaling and Heart Development Developmental Cell 2020-11-01 [PMID: 32966817] |
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| Sidorov MS, Kim H, Rougie M et al. Visual Sequences Drive Experience-Dependent Plasticity in Mouse Anterior Cingulate Cortex Cell Reports 2020-09-15 [PMID: 32937128] (Immunohistochemistry) |
Immunohistochemistry |
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| Donato C, Kunz L, Castro-Giner F et al. Hypoxia Triggers the Intravasation of Clustered Circulating Tumor Cells Cell reports 2020-09-08 [PMID: 32905777] (KD, ICC/IF, WB) |
KD, ICC/IF, WB |
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WB |
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| Lourds M Fernando, Jeandele Elliot, Anna K Allen The Caenorhabditis elegans proteasome subunit RPN-12 is required for hermaphrodite germline sex determination and oocyte quality. Developmental dynamics : an official publication of the American Association of Anatomists 2022-01-18 [PMID: 32767462] |
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ICC/IF |
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| Saha C, Mohanraju P, Stubbs A et al. Guide-free Cas9 from pathogenic Campylobacter jejuni bacteria causes severe damage to DNA Sci Adv 2020-06-01 [PMID: 32596446] (WB) |
WB |
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| Cuijpers SAG, Willemstein E, Ruppert JG et al. Chromokinesin KIF4A teams up with Stathmin 1 to regulate abscission in a SUMO-dependent manner J. Cell. Sci. 2020-06-26 [PMID: 32591481] (WB) |
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| Shin E Y, Park J H et al. Integrin-mediated adhesions in regulation of cellular senescence. Sci Adv 2020-01-05 [PMID: 32494696] (WB, IF/IHC) |
WB, IF/IHC |
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| Min H, Youn E, Shim YH Maternal Caffeine Intake Disrupts Eggshell Integrity and Retards Larval Development by Reducing Yolk Production in a Caenorhabditis elegans Model Nutrients 2020-05-07 [PMID: 32392893] (WB) |
WB |
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| Chen Z, Wang ZH, Zhang G et al. Mitochondrial DNA segregation and replication restrict the transmission of detrimental mutation J. Cell Biol. 2020-07-06 [PMID: 32375181] (ICC/IF) |
ICC/IF |
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WB |
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| Monette A, Niu M, Chen L et al. Pan-retroviral Nucleocapsid-Mediated Phase Separation Regulates Genomic RNA Positioning and Trafficking Cell Rep 2020-04-23 [PMID: 32320662] (WB) |
WB |
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| Kashiwagi H, Ishimoto H, Izumi SI et al. Human PZP and common marmoset A2ML1 as pregnancy related proteins Sci Rep 2020-03-22 [PMID: 32198464] (IF/IHC) |
IF/IHC |
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| Hu X, Deng Q, Ma L et al. Meningeal lymphatic vessels regulate brain tumor drainage and immunity Cell Res 2020-02-26 [PMID: 32094452] (IHC-Fr) |
IHC-Fr |
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| Tsukamoto T, Gearhart M D et al. Insights into the Involvement of Spliceosomal Mutations in Myelodysplastic Disorders from Analysis of SACY-1/DDX41 in Caenorhabditis elegans. Genetics 2020-01-04 [PMID: 32060018] (WB) |
WB |
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| Corbet C, Bastien E, Santiago de Jesus JP et al. TGFbeta2-induced formation of lipid droplets supports acidosis-driven EMT and the metastatic spreading of cancer cells Nat Commun 2020-01-23 [PMID: 31974393] (IF/IHC) |
IF/IHC |
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| Dubey R, Stivala CE, Nguyen HQ et al. Lipid droplets can promote drug accumulation and activation Nat. Chem. Biol. 2020-02-01 [PMID: 31932720] (WB) |
WB |
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WB |
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ICC/IF |
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| Wang Z H, Liu Y et al. Electron transport chain biogenesis activated by a JNK-insulin-Myc relay primes mitochondrial inheritance in Drosophila. Elife 2019-10-15 [PMID: 31612862] (IF/IHC) |
IF/IHC |
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WB |
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WB |
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| Shi C, Pan FC, Kim JN et al. Differential Cell Susceptibilities to Kras(G12D) in the Setting of Obstructive Chronic Pancreatitis Cell Mol Gastroenterol Hepatol 2019-01-01 [PMID: 31310834] (IF/IHC) |
IF/IHC |
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Lee M, Hwang Y S et al. Developmentally regulated GTP-binding protein 1 modulates ciliogenesis via an interaction with Dishevelled. J Cell Biol 2019-03-07 [PMID: 31270137] (IP, IF/IHC)
Details:
Xenopus laevis (African clawed frog) |
IP, IF/IHC |
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| Nilangekar K, Murmu N, Sahu G et al. Generation and Characterization of Germline-Specific Autophagy and Mitochondrial Reactive Oxygen Species Reporters in Drosophila Front Cell Dev Biol 2019-04-20 [PMID: 31001531] (WB) |
WB |
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WB |
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WB |
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IF/IHC |
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WB |
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| Zhao S, Fortier TM, Baehrecke EH. Autophagy Promotes Tumor-like Stem Cell Niche Occupancy Curr Biol 2018-10-02 [PMID: 30270184] (ICC/IF) |
ICC/IF |
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ICC/IF |
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WB |
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| Wang L, Chen SR, Ma H et al. Regulating nociceptive transmission by VGluT2-expressing spinal dorsal horn neurons J Neurochem 2018-11-01 [PMID: 30203849] (IF/IHC) |
IF/IHC |
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| Sebastiaan De Schepper, Simon Verheijden, Javier Aguilera-Lizarraga, Maria Francesca Viola, Werend Boesmans, Nathalie Stakenborg, Iryna Voytyuk, Inga Schmidt, Bram Boeckx, Isabelle Dierckx de Casterlé, Veerle Baekelandt, Erika Gonzalez Dominguez, Matthias Mack, Inge Depoortere, Bart De Strooper, Ben Sprangers, Uwe Himmelreich, Stefaan Soenen, Martin Guilliams, Pieter Vanden Berghe, Elizabeth Jones, Diether Lambrechts, Guy Boeckxstaens Self-Maintaining Gut Macrophages Are Essential for Intestinal Homeostasis. Cell 2019-06-14 [PMID: 30173915] |
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ICC/IF |
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| Yao H, Price TT, Cantelli G et al. Leukaemia hijacks a neural mechanism to invade the central nervous system Nature 2018-07-20 [PMID: 30022166] (IF/IHC) |
IF/IHC |
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| Nakano Y, Kelly MC, Rehman AU et al. Defects in the Alternative Splicing-Dependent Regulation of REST Cause Deafness Cell 2018-07-03 [PMID: 29961578] (ICC/IF) |
ICC/IF |
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| Beer-Hammer S, Lee SC, Mauriac SA et al. Gai Proteins are Indispensable for Hearing. Cell. Physiol. Biochem. 2018-06-21 [PMID: 29940568] (IF/IHC) |
IF/IHC |
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| Bell P, Yu H, Kuntz L et al. Preparation of Nonhuman Primate Eyes for Histological Evaluation After Retinal Gene Transfer Hum Gene Ther Methods 2018-06-01 [PMID: 29848071] (IF/IHC) |
IF/IHC |
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IF/IHC |
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| Herrera LA, Starr DA The E3 Ubiquitin Ligase MIB-1 Is Necessary To Form the Nuclear Halo in Caenorhabditis elegans Sperm G3 (Bethesda) 2018-07-02 [PMID: 29776970] (IF/IHC) |
IF/IHC |
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WB, ICC/IF |
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IP |
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ICC/IF |
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IP, WB |
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IF/IHC |
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| Yoon S, Kawasaki I, Shim YH The B-type cyclin CYB-1 maintains the proper position and number of centrosomes during spermatogenesis in Caenorhabditis elegans J Cell Sci 2017-08-15 [PMID: 28705837] (ICC/IF) |
ICC/IF |
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ELISA |
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| Patra C, Kontarakis Z, Kaur H et al. The zebrafish ventricle: A hub of cardiac endothelial cells for in vitro cell behavior studies Sci Rep 2017-06-05 [PMID: 28578380] (IF/IHC) |
IF/IHC |
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| Tsukamoto T, Gearhart MD, Spike CA et al. LIN-41 and OMA Ribonucleoprotein Complexes Mediate a Translational Repression-to-Activation Switch Controlling Oocyte Meiotic Maturation and the Oocyte-to-Embryo Transition in Caenorhabditis elegans Genetics 2017-01-01 [PMID: 28576864] (WB) |
WB |
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| Knutson AK, Egelhofer T, Rechtsteiner A, Strome S Germ Granules Prevent Accumulation of Somatic Transcripts in the Adult Caenorhabditis elegans Germline Genetics 2017-01-01 [PMID: 28258184] (ICC/IF) |
ICC/IF |
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ICC/IF |
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| Davidson R, Pontasch JA, Wu JQ. Sbg1 Is a Novel Regulator for the Localization of the beta-Glucan Synthase Bgs1 in Fission Yeast PLoS One 2016-11-30 [PMID: 27898700] (IP, WB) |
IP, WB |
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| Yovchev MI, Oertel M. Fetal Liver Stem/Progenitor Cell Transplantation: A Model to Study Tissue Mass Replacement and Cell-Based Therapies. Methods Mol. Biol. 2016-11-10 [PMID: 27830548] |
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| Bao K, Carr T, Wu J et al. BATF Modulates the Th2 Locus Control Region and Regulates CD4+ T Cell Fate during Antihelminth Immunity J Immunol 2016-11-01 [PMID: 27798167] (IF/IHC) |
IF/IHC |
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IHC-P |
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| Ahn JH, Rechsteiner A, Strome S, Kelly WG. A Conserved Nuclear Cyclophilin Is Required for Both RNA Polymerase II Elongation and Co-transcriptional Splicing in Caenorhabditis elegans. PLoS Genet. 2016-08-01 [PMID: 27541139] (IP, ICC/IF) |
IP, ICC/IF |
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| Liu Y, Lee IJ, Sun M et al. Roles of the novel coiled-coil protein Rng10 in septum formation during fission yeast cytokinesis Mol Biol Cell 2016-07-08 [PMID: 27385337] (IP) |
IP |
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| Barrionuevo FJ, Hurtado A, Kim GJ et al. Sox9 and Sox8 protect the adult testis from male-to-female genetic reprogramming and complete degeneration Elife 2016-06-21 [PMID: 27328324] (IF/IHC) |
IF/IHC |
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| Zeng Y, Guo W, Xu G et al. Xyloketal-derived small molecules show protective effect by decreasing mutant Huntingtin protein aggregates in Caenorhabditis elegans model of Huntington's disease Drug Des Devel Ther 2016-04-13 [PMID: 27110099] (WB) |
WB |
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| Myeku N, Clelland CL, Emrani S et al. Tau-driven 26S proteasome impairment and cognitive dysfunction can be prevented early in disease by activating cAMP-PKA signaling. Nat. Med. 2016-01-01 [PMID: 26692334] (WB) |
WB |
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| Sorrentino NC, Maffia V, Strollo S et al. A Comprehensive Map of CNS Transduction by Eight Recombinant Adeno-associated Virus Serotypes Upon Cerebrospinal Fluid Administration in Pigs. Mol. Ther. 2015-12-07 [PMID: 26639405] (WB, IHC-Fr, IHC-FrFl) |
WB, IHC-Fr, IHC-FrFl |
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| Gross S, Balderes D, Liu J et al. Nkx2.2 is expressed in a subset of enteroendocrine cells with expanded lineage potential. Am J Physiol Gastrointest Liver Physiol 2015-12-15 [PMID: 26492922] |
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| Rueckemann JW, DiMauro AJ, Rangel LM et al. Transient optogenetic inactivation of the medial entorhinal cortex biases the active population of hippocampal neurons Hippocampus 2015-08-25 [PMID: 26299904] (IHC-Fr) |
IHC-Fr |
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| Akhtar Ali M, Younis S, Wallerman O et al. Transcriptional modulator ZBED6 affects cell cycle and growth of human colorectal cancer cells Proc Natl Acad Sci U S A 2015-06-10 [PMID: 26056301] (WB) |
WB |
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| Gautam M, Fujita D, Kimura K et al. Transplantation of adipose tissue-derived stem cells improves cardiac contractile function and electrical stability in a rat myocardial infarction model J Mol Cell Cardiol 2015-03-01 [PMID: 25724725] (IHC-P) |
IHC-P |
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| Doi K, Ogata F, Eto H et al. Differential contributions of graft- and host-derived cells in tissue regeneration/remodeling after fat grafting Plast. Reconstr. Surg. 2015-02-20 [PMID: 25724061] (IHC-P) |
IHC-P |
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| Matthews DW, Deschenes M, Furuta T et al. Feedback in the brainstem: an excitatory disynaptic pathway for control of whisking J Comp Neurol 2015-04-15 [PMID: 25503925] (IF/IHC) |
IF/IHC |
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| Li M, Bian C, Yu X. Poly(ADP-ribosyl)ation is recognized by ECT2 during mitosis. Cell Cycle 2014-01-01 [PMID: 25486481] (WB) |
WB |
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Davidson R, Laporte D, Wu JQ. Regulation of Rho GEF Rgf3 by the arrestin Art1 in fission yeast cytokinesis. Mol. Biol. Cell. 2014-12-03 [PMID: 25473118] (IP)
Details:
GFP antibody used in IP for pulling down the YFP-tagged protein expressed at its native level from fission yeast cell extracts (Figure 3E) |
IP |
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| Meng L, Ward AJ, Chun S et al. Towards a therapy for Angelman syndrome by targeting a long non-coding RNA. Nature. 2014-12-01 [PMID: 25470045] (WB) |
WB |
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| Liu D, Yovchev Mi, Zhang J et al. Identification and Characterization of Mesenchymal-Epithelial Progenitor-Like Cells in normal and Injured Rat Liver. Am. J. Pathol. 2014-11-06 [PMID: 25447047] |
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WB |
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| Nelson C, Ambros V, Baehrecke Eh. miR-14 Regulates Autophagy during Developmental Cell Death by Targeting ip3-kinase 2. Mol. Cell. 2014-10-08 [PMID: 25306920] (WB) |
WB |
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| Bratton B, Wright Cw. NF-kB-inducing kinase (nIK) contains an amino terminal inhibitor of apoptosis (IAP) binding motif (IBM) that potentiates nIK degradation by c-IAP1* J. Biol. Chem. et al. 2014-09-22 [PMID: 25246529] (WB) |
WB |
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Cain NE, Tapley EC, McDonald KL et al. The SUN protein UNC-84 is required only in force-bearing cells to maintain nuclear envelope architecture. J. Cell Biol. 2014-07-21 [PMID: 25023515] (IHC-WhMt)
Details:
GFP antibody used for immunofluorescence staining of C elegans embryos - antibody diluted 1:200 in UNC-83 antiserum for simultaneous staining of KASH and SUN chimeric proteins. |
IHC-WhMt |
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| Shigunov Patricia, Sotelo-Silveira Jose, Stimamiglio Marco Augusto et al. Ribonomic analysis of human DZIP1 reveals its involvement in ribonucleoprotein complexes and stress granules. BMC Mol Biol. 2014-07-03 [PMID: 24993635] (IP) |
IP |
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| Wang N, Lo Presti L, Zhu YH et al. The novel proteins Rng8 and Rng9 regulate the myosin-V Myo51 during fission yeast cytokinesis. J. Cell Biol. 2014-05-12 [PMID: 24798735] (IP) |
IP |
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| Buzgariu W, Crescenzi M, Galliot B. Robust G2 pausing of adult stem cells in Hydra. Differentiation 2014-04-21 [PMID: 24703763] (IHC-WhMt, ICC/IF) |
IHC-WhMt, ICC/IF |
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| Walter TJ, Vanderpool C, Cast AE, Huppert SS. Intrahepatic Bile Duct Regeneration in Mice Does Not Require Hnf6 or Notch Signaling through Rbpj. Am. J. Pathol. 2014-03-13 [PMID: 24631193] (IHC-P) |
IHC-P |
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| Klos KS, Warmka JK, Drachenberg DM et al. Building Bridges toward Invasion: Tumor Promoter Treatment Induces a Novel Protein Kinase C-Dependent Phenotype in MCF10A Mammary Cell Acini. PLoS ONE 2014-03-06 [PMID: 24599099] (ICC/IF) |
ICC/IF |
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| Montgomery Scott R, Nargizyan Taya, Meliton Vicente et al. A novel osteogenic oxysterol compound for therapeutic development to promote bone growth: activation of hedgehog signaling and osteogenesis through smoothened binding. J Bone Miner Res. 2014-08-01 [PMID: 24591126] |
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| Hagiwara M, Kokubu E, Sugiura S et al. Vinculin and Rab5 Complex Is Requited for Uptake of Staphyrococcus aureus and Interleukin-6 Expression. PLoS ONE 2014-01-27 [PMID: 24466349] (IP, WB) |
IP, WB |
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| Tian G, Ropelewski P, Nemet I et al. An unconventional secretory pathway mediates the Cilia targeting of peripherin/rds. J. Neurosci. 2014-01-15 [PMID: 24431457] (WB) |
WB |
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| Nachtergaele S, Whalen DM, Mydock LK et al. Structure and function of the Smoothened extracellular domain in vertebrate Hedgehog signaling. Elife. 2013-10-29 [PMID: 24171105] (WB) |
WB |
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| Oldenbroek M, Robertson SM, Guven-Ozkan T et al. Regulation of maternal Wnt mRNA translation in C. elegans embryos. Development. 2013-11-01 [PMID: 24131629] (IP) |
IP |
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| Hullinger R, Ugalde J, Puron-Sierra L et al. The MRI contrast agent gadoteridol enhances distribution of rAAV1 in the rat hippocampus. Gene Ther. 2013-09-19 [PMID: 24048419] (IHC-Fr) |
IHC-Fr |
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| Zhu YH, Ye Y, Wu Z et al. Cooperation between Rho-GEF Gef2 and its binding partner Nod1 in the regulation of fission yeast cytokinesis Mol Biol Cell 2013-08-23 [PMID: 23966468] (IP) |
IP |
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| Park BH, Kook S, Lee S et al. An isoform of C/EBPbeta, LIP, regulates expression of the chemokine receptor CXCR4 and modulates breast cancer cell migration. J Biol Chem. 2013-10-04 [PMID: 23966000] (IHC-P) |
IHC-P |
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| Chang TK, Shravage BV, Hayes SD et al. Uba1 functions in Atg7- and Atg3-independent autophagy Nat Cell Biol 2013-07-23 [PMID: 23873149] (EM) |
EM |
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| Huang WC, Yang CC, Chen IH et al. Treatment of Glucocorticoids Inhibited Early Immune Responses and Impaired Cardiac Repair in Adult Zebrafish PLoS One 2013-06-28 [PMID: 23805247] (IHC-Fr, ICC/IF) |
IHC-Fr, ICC/IF |
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| Wang G, Li Y, Wang XY et al. Slit/Robo1 signaling regulates neural tube development by balancing neuroepithelial cell proliferation and differentiation Exp Cell Res 2013-02-27 [PMID: 23438940] (IF/IHC, ICC/IF) |
IF/IHC, ICC/IF |
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| Yang JR, Hsu CW, Liao SC et al. Transplantation of embryonic stem cells improves the regeneration of periodontal furcation defects in a porcine model J Clin Periodontol 2013-02-26 [PMID: 23431981] (IHC-P) |
IHC-P |
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| Shravage BV, Hill JH, Powers CM et al. Atg6 is required for multiple vesicle trafficking pathways and hematopoiesis in Drosophila Development 2013-02-15 [PMID: 23406899] (IHC-P) |
IHC-P |
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| Vergara MN, Gutierrez C, O'Brien DR et al. Ex vivo electroporation of retinal cells: a novel, high efficiency method for functional studies in primary retinal cultures Exp Eye Res 2013-02-02 [PMID: 23370269] (ICC/IF) |
ICC/IF |
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| Shinojima N, Hossain A, Takezaki T et al. TGF-beta mediates homing of bone marrow-derived human mesenchymal stem cells to glioma stem cells Cancer Res 2013-02-01 [PMID: 23365134] (ICC/IF) |
ICC/IF |
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| Wu Y, Wang K, Karapetyan A et al. Connective Tissue Fibroblast Properties Are Position-Dependent during Mouse Digit Tip Regeneration. PLoS One 2013-01-01 [PMID: 23349966] (ICC/IF, IF/IHC) |
ICC/IF, IF/IHC |
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| Hauser J, Verma-Gaur J, Grundstrom T Broad feedback inhibition of pre-B-cell receptor signaling components Mol Immunol 2013-01-01 [PMID: 23318223] |
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| Cho JH, Kam JW, Cloutier JF Slits and Robo-2 regulate the coalescence of subsets of olfactory sensory neuron axons within the ventral region of the olfactory bulb Dev Biol 2012-11-15 [PMID: 22981605] (ICC/IF, IF/IHC) |
ICC/IF, IF/IHC |
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| Xia L, Yin S, Wang J Inner ear gene transfection in neonatal mice using adeno-associated viral vector: a comparison of two approaches PLoS One 2012-01-01 [PMID: 22912830] (IHC-Fr) |
IHC-Fr |
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| Georg I, Barrionuevo F, Wiech T, Scherer G. Sox9 and Sox8 Are Required for Basal Lamina Integrity of Testis Cords and for Suppression of FOXL2 During Embryonic Testis Development in Mice Biol Reprod 2012-10-25 [PMID: 22837482] |
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WB |
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| Lin CS, Lin FY, Ho LJ et al. PKCdelta signalling regulates SR-A and CD36 expression and foam cell formation Cardiovasc Res 2012-06-13 [PMID: 22687273] (WB) |
WB |
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| Ahmed M, Xu J, Xu PX EYA1 and SIX1 drive the neuronal developmental program in cooperation with the SWI/SNF chromatin-remodeling complex and SOX2 in the mammalian inner ear Development 2012-01-01 [PMID: 22513373] (IF/IHC) |
IF/IHC |
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| Jin H, Zangar RC. High-throughput, multiplexed analysis of 3-nitrotyrosine in individual proteins. Curr Protoc Toxicol 2012-02-01 [PMID: 22511115] |
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| Meng L, Person RE, Beaudet AL Ube3a-ATS is an atypical RNA polymerase II transcript that represses the paternal expression of Ube3a Hum Mol Genet 2012-07-01 [PMID: 22493002] (ICC/IF) |
ICC/IF |
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| Powell AE, Wang Y, Li Y et al. The pan-ErbB negative regulator Lrig1 is an intestinal stem cell marker that functions as a tumor suppressor Cell 2012-03-30 [PMID: 22464327] (IHC-Fr, ICC/IF) |
IHC-Fr, ICC/IF |
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| Lee IJ, Wu JQ. Characterization of Mid1 domains for targeting and scaffolding in fission yeast cytokinesis. J Cell Sci 2012-06-15 [PMID: 22427686] (WB) |
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| Ahmed M, Wong EY, Sun J et al. Eya1-six1 interaction is sufficient to induce hair cell fate in the cochlea by activating atoh1 expression in cooperation with sox2. Dev Cell;22(2):377-90. 2012-02-14 [PMID: 22340499] |
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| Canton J, Ndjamen B, Hatsuzawa K, Kima PE. Disruption of the fusion of Leishmania parasitophorous vacuoles with ER vesicles results in the control of the infection. Cell Microbiol doi: 10.1111/j.1462-5822.2012.01767.x. 2012-02-06 [PMID: 22309219] |
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| Ye Y, Lee IJ, Runge KW et al. Roles of putative Rho-GEF Gef2 in division-site positioning and contractile-ring function in fission yeast cytokinesis Mol Biol Cell 2012-02-03 [PMID: 22298427] (IP) |
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| Nachtergaele S, Mydock LK, Krishnan K et al. Oxysterols are allosteric activators of the oncoprotein Smoothened. Nat Chem Biol 2012-02-01 [PMID: 22231273] (WB) |
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| Venters SJ, Cuenca PD, Hyer J et al. Retinal and anterior eye compartments derive from a common progenitor pool in the avian optic cup. Mol Vis 2011-01-01 [PMID: 22219630] |
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| Huang H-S, Allen JA, Mabb AM et al. Topoisomerase inhibitors unsilence the dormant allele of Ube3a in neurons. Nature. 2011-12-21 [PMID: 22190039] |
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| Liang H-E, Reinhardt RL, Bando JK et al. Divergent expression patterns of IL-4 and IL-13 define unique functions in allergic immunity. Nature Immunology. 2011-12-04 [PMID: 22138715] |
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| Fang L, Green SR, Baek JS et al. In vivo visualization and attenuation of oxidized lipid accumulation in hypercholesterolemic zebrafish. J Clin Invest. 2011-11-21 [PMID: 22105168] |
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| Pane A, Jiang P, Zhao DY et al. The Cutoff protein regulates piRNA cluster expression and piRNA production in the Drosophila germline. The EMBO Journal. 2011-09-27 [PMID: 21952049] |
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| Tian H, Biehs B, Warming S et al. A reserve stem cell population in small intestine renders Lgr5-positive cells dispensable. Nature. 2011-09-18 [PMID: 21927002] |
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| Ninagawa N, Murakami R, Isobe E et al. Mesenchymal stem cells originating from ES cells show high telomerase activity and therapeutic benefits. Differentiation. 2011-08-18 [PMID: 21855203] |
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| Schmidt FI, Bleck CK, Helenius A et al. Vaccinia extracellular virions enter cells by macropinocytosis and acid-activated membrane rupture. EMBO J 2011-07-01 [PMID: 21792173] |
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| Damulewicz M, Pyza E. The clock input to the first optic neuropil of Drosophila melanogaster expressing neuronal circadian plasticity. PLoS One 2011-01-01 [PMID: 21760878] |
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| Safina A, Sotomayor P, Limoge M et al. TAK1-TAB2 signaling contributes to bone destruction by breast carcinoma cells. Mol Cancer Res. 2011-06-02 [PMID: 21700681] |
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| Wang H, Murphy R, Taaffe D et al. Efficient cochlear gene transfection in guinea-pigs with adeno-associated viral vectors by partial digestion of round window membrane. Gene Ther 2012-03-01 [PMID: 21697953] (ICC/IF) |
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| Chen X, Nelson CD, Li X et al. PSD-95 is required to sustain the molecular organization of the postsynaptic density J Neurosci 2011-04-29 [PMID: 21525273] |
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| Laporte D, Coffman VC, Lee IJ, Wu JQ. Assembly and architecture of precursor nodes during fission yeast cytokinesis. J Cell Biol 2011-03-21 [PMID: 21422229] (IP) |
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| Ray KC, Bell KM, Yan J et al. Epithelial tissues have varying degrees of susceptibility to Kras(G12D)-initiated tumorigenesis in a mouse model PLoS One 2011-02-02 [PMID: 21311774] (IF/IHC) |
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| Sparks EE, Perrien DS, Huppert KA et al. Defects in hepatic Notch signaling result in disruption of the communicating intrahepatic bile duct network in mice Dis Model Mech 2011-01-01 [PMID: 21282722] (IF/IHC, ICC/IF) |
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| Torres J, Funk HM, Zegers MM et al. The syntaxin 4 N terminus regulates its basolateral targeting by munc18c-dependent and -independent mechanisms. J Biol Chem 2011-03-01 [PMID: 21278252] |
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| S Dindot, P Piccolo, NC Grove et al. Intrathecal injection of HDAd vectors results in long-term transgene expression in neuroependymal cells and neurons. Hum Gene Ther. 2010-12-22 [PMID: 21175294] |
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| Papadopoulos C, Arato K, Lilienthal E et al. Splice variants of the dual specificity tyrosine phosphorylation-regulated kinase 4 (DYRK4) differ in their subcellular localization and catalytic activity. J Biol Chem 2011-02-01 [PMID: 21127067] |
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| Fridolfsson HN, Starr DA. Kinesin-1 and dynein at the nuclear envelope mediate the bidirectional migrations of nuclei. J Cell Biol 2010-10-04 [PMID: 20921138] (ICC/IF) |
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| Xue X, Jaulin F, Espenel C et al. PH-domain-dependent selective transport of p75 by kinesin-3 family motors in non-polarized MDCK cells. J Cell Sci 2010-05-01 [PMID: 20427314] (IP) |
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| Jaramillo-Lambert A, Engebrecht J. A Single Unpaired and Transcriptionally Silenced X Chromosome Locally Precludes Checkpoint Signaling in the Caenorhabditis elegans Germ Line. Genetics. [PMID: 20008570] |
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| Shafikhani, HS, Engel, Joanne. Pseudomonas aeruginosa type III-secreted toxin ExoT inhibits host-cell division by targeting cytokinesis at multiple steps. PNAS 103:15605-15610. 2006-01-01 [PMID: 17030800] |
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| Schr�der C, Zissel L, Mersiowsky S et al. An EOMES induced epigenetic deflection initiates lineage commitment at mammalian gastrulation bioRxiv 2023-03-17 (WB, IP, Human) |
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| Fridolfsson HN, Ly N, Meyerzon M, Starr DA. UNC-83 coordinates kinesin-1 and dynein activities at the nuclear envelope during nuclear migration. Dev Biol 2010-02-15 [PMID: 20005871] (WB, ICC/IF) |
WB, ICC/IF |
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| Tokui K, Adachi H, Waza M et al. 17-DMAG ameliorates polyglutamine-mediated motor neuron degeneration through well-preserved proteasome function in an SBMA model mouse. Hum Mol Genet;18(5):898-910. 2009-01-01 [PMID: 19066230] |
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| Hoffman GE, Le WW, Sita LV. The importance of titrating antibodies for immunocytochemical methods. Curr Protoc Neurosci. 2008-10-01 [PMID: 18972376] |
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| Tretyakova I, Zolotukhin AS, Tan W et al. Nuclear export factor family protein participates in cytoplasmic mRNA trafficking. J Biol Chem 2005-09-09 [PMID: 16014633] |
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| Day YJ, Li Y, Rieger JM et al. A2A adenosine receptors on bone marrow-derived cells protect liver from ischemia-reperfusion injury J Immunol 2005-04-09 [PMID: 15814735] |
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| Green CB, Zhao X, Hoyer LL. Use of green fluorescent protein and reverse transcription-PCR to monitor Candida albicans agglutinin-like sequence gene expression in a murine model of disseminated candidiasis. Infect Immun 2005-03-01 [PMID: 15731087] |
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| Kanazawa, Y, Verma, IM. Little evidence of bone moarrow-derived hepatocytes in the replacement of injured liver. PNAS 100:11850-11853. 2003-01-01 [PMID: 12920184] |
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| Meyerzon M, Fridolfsson HN, Ly N et al. UNC-83 is a nuclear-specific cargo adaptor for kinesin-1-mediated nuclear migration. Development;136(16):2725-2733. 2009-01-01 [PMID: 9605495] |
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| Radel S Investigating post-translational modification of cyclin D1 in breast cancer Thesis 2023-06-26 (WB, IP) |
WB, IP |
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Odahara M, Ara M, Nakagawa R et al. A multiple shoot induction system for peptide-mediated gene delivery into plastids in <i>Arabidopsis thaliana</i>, <i>Nicotiana benthamiana</i>, and <i>Fragaria</i>×<i>ananassa</i> Plant Biotechnology 2023-09-07 (WB)
Details:
Dilution: 1:1000 |
WB |
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| Reinhardt, R L, Hong, S, Kang, S-J et al. Visualization of IL-12/23p40 in vivo reveals immunostimulatory dendritic cell migrants that promote Th1 differentiation. J Immunol 177:1618-1627. 2006-01-01 [PMID: 16849470] |
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| Groff KJ Generation of focal Depdc5 knockout mouse model and implications for focal epilepsy Thesis 2023-01-01 (WB) |
WB |
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| Samuel T In Vivo Effects of Stereotactic Body Radiation Therapy on the Pancreatic Tumor Microenvironment Thesis 2023-01-01 |
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| Zhukovskaya A, Zimmerman C, Willmore L et al. Differences in an aversive teaching signal produce brainwide and behavioral substrates of susceptibility bioRxiv 2023-11-06 |
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| Fang X, Nie L, Putluri S et al. Sertoli Cell-Specific Activation of Transforming Growth Factor Beta Receptor 1 Leads to Testicular Granulosa Cell Tumor Formation Cells 2023-11-27 |
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| Steeg C, Adler G, Sparwasser T et al. Limited role of CD4+Foxp3+ regulatory T cells in the control of experimental cerebral malaria. J Immunol;183(11):7014-22. 2009-12-01 [PMID: 19890049] |
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| Bowman AB, Yoo SY, Dantuma NP et al. Neuronal dysfunction in a polyglutamine disease model occurs in the absence of ubiquitin-proteasome system impairment and inversely correlates with the degree of nuclear inclusion formation. Hum Mol Genet;14(5):679-91. 2005-03-01 [PMID: 15661755] |
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| Goetsch PD, Strome S DREAM Interrupted: Severing MuvB from DREAM's pocket protein impairs gene repression but not DREAM assembly on chromatin BioRxiv 2019-06-13 |
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| Yu D, Maroteaux M, Song Y et al. The transcription factor Shox2 shapes thalamocortical neuron firing properties by regulation of key ion channels bioRxiv (WB, IF/IHC) |
WB, IF/IHC |
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| Dufeys C Exploring the role of fibroblastic AMPKalpha1 in ventricular remodelling after myocardial infarction: implication of connexin 43 and miR-125b Thesis 2019-01-01 |
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| Chowdhury, A Tamalin/GRAS-1 Connects Glutamate Receptor Activity to the Insulin/IGF Signaling Cascade to Regulate Neuroprotection in a Nematode Model of Excitotoxicity Thesis 2020-01-01 (IP, C. elegans) |
IP |
C. elegans |
| Steward O, Yee K, Metcalfe M et al. Rostro-caudal specificity of corticospinal tract projections in mice bioRxiv Jul 22 2020 (IHC) |
IHC |
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| Cherian JR Characterizing the Mechanisms of Ectopic Expression of Dream Complex Target Genes and the HTA Phenotype in C. Elegans Thesis 2020-01-01 (C. elegans) |
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C. elegans |
| Meyerzon M, Fridolfsson HN, Ly N et al. UNC-83 is a nuclear-specific cargo adaptor for kinesin-1-mediated nuclear migration Development 2009-07-17 [PMID: 19605495] (ICC/IF) |
ICC/IF |
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| Green EL Nicotinamide, neural cells, and Parkinsons disease: exploring nicotinamides effects on proliferation, neuronal differentiation, and neuroprotection within in vitro models Thesis 2021-01-01 |
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| Shafikhani SH, Mostov K, Engel J. Focal adhesion components are essential for mammalian cell cytokinesis Cell Cycle 2008-09-13 [PMID: 18787414] (WB) |
WB |
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Show All 279 Publications.
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![Total cell lysates (20ug) with protein of interest tagged with GFP was loaded. Blotted with GFP antibody [HRP] NB600-308H, without any secondary antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/GFP-Antibody-Western-Blot-NB600-308-img0036.jpg "Total cell lysates (20ug) with protein of interest tagged with GFP was loaded. Blotted with GFP antibody [HRP] NB600-308H, without any secondary antibody. Image from verified customer review.")
![Western Blot of GFP antibody. Lane 1: Wild type GFP (0.1 ug) was used to spike HeLa whole cell lysate. Lane 2: none. Load: 30 ug per lane.Primary antibody: GFP antibody at 1:1000 for overnight at 4C.Secondary antibody: IRDye800(TM) Goat-a-Rabbit IgG [H&L] MX10 at 1:10,000 for 45 min at RT.Block: 5% BLOTTO in PBS overnight at 4C.Predicted/Observed size: 27 kDa for epitope tag GFP. Other band(s): none.](http://images.novusbio.com/fullsize/GFP-Antibody-Western-Blot-NB600-308-img0032.jpg "Western Blot of GFP antibody. Lane 1: Wild type GFP (0.1 ug) was used to spike HeLa whole cell lysate. Lane 2: none. Load: 30 ug per lane.Primary antibody: GFP antibody at 1:1000 for overnight at 4C.Secondary antibody: IRDye800(TM) Goat-a-Rabbit IgG [H&L] MX10 at 1:10,000 for 45 min at RT.Block: 5% BLOTTO in PBS overnight at 4C.Predicted/Observed size: 27 kDa for epitope tag GFP. Other band(s): none.")
![Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB600-308] - Myc is post-transcriptionally regulated by the IIS-GSK3 cascade.(A) Germaria from ovaries of ctrl, chicoRNAi, & bskRNAi driven by nos-Gal4 expressing LacZ under the control of myc endogenous promoter stained with anti-beta -galactosidase & anti-1B1. Scale bars, 10 μm. (B) A representative live imaging of germaria from flies expressing GFP-tagged Sgg under its endogenous promoter. Scale bar, 10 μm. (C) A germarium from wt flies endogenously expressing Myc-GFP stained with anti-GFP (green) & anti-p-GSK3 (red). Germarium regions are indicated. Myc protein highly corresponds to inhibited form of GSK3. Scale bar, 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31612862), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-308_rabbit-polyclonal-gfp-antibody-31020241529193.jpg "Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB600-308] - Myc is post-transcriptionally regulated by the IIS-GSK3 cascade.(A) Germaria from ovaries of ctrl, chicoRNAi, & bskRNAi driven by nos-Gal4 expressing LacZ under the control of myc endogenous promoter stained with anti-beta -galactosidase & anti-1B1. Scale bars, 10 μm. (B) A representative live imaging of germaria from flies expressing GFP-tagged Sgg under its endogenous promoter. Scale bar, 10 μm. (C) A germarium from wt flies endogenously expressing Myc-GFP stained with anti-GFP (green) & anti-p-GSK3 (red). Germarium regions are indicated. Myc protein highly corresponds to inhibited form of GSK3. Scale bar, 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31612862), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.")
![Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB600-308] - Myc protein pattern in the ovary.(A) A representative low-magnification image of ovarioles from flies endogenously expressing Myc-GFP stained with anti-GFP, anti-1B1, & DAPI. Note that Myc level is low in early germarium stages, becomes high from germarium region 2B, & reduces from the stage-10 egg chamber. Scale bar, 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31612862), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-308_rabbit-polyclonal-gfp-antibody-310202415291968.jpg "Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB600-308] - Myc protein pattern in the ovary.(A) A representative low-magnification image of ovarioles from flies endogenously expressing Myc-GFP stained with anti-GFP, anti-1B1, & DAPI. Note that Myc level is low in early germarium stages, becomes high from germarium region 2B, & reduces from the stage-10 egg chamber. Scale bar, 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31612862), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.")
![Western Blot: GFP Antibody [NB600-308] - MISP interacts & co-localizes with IQGAP1. (a) HEK293T cells transiently overexpressing GFP-IQGAP1 & FLAG-MISP were used for co-immunoprecipitation experiments from both sides (GFP trap or FLAG M2 beads). (b) Endogenous immunoprecipitation using IQGAP1 antibody or control immunoglobulin (IgG) was carried out in HeLa cell lysates & MISP was detected in the eluate. (c) In vitro interaction between purified MBP-MISP & GST-IQGAP1 was detected by GST & MBP pull-down experiments. (d) Immunoprecipiation experiments showing that GFP-MISP binds endogenous IQGAP1 (upper panel) & MISP binds to IQGAP1 endogenously using specific antibodies (lower panel) in mitotically arrested (taxol & nocodazole, respectively) HeLa cell lysates. (a–d) Images were gained from the same Western blot membrane after cutting/cropping & presented with different exposure times or contrast enhancement for better presentation purposes. The dividing lane marks the grouping of images of the same (or different) membrane. Uncropped blots, where applicable, are included in Supplementary Fig. 4. (e) HeLa cells were immunostained for MISP & IQGAP1 & co-localization was visualized in mitosis in single-plane confocal images, scale bar: 5 μm. Lower pictures: After manual thresholding white spots mark the co-localizing areas in the merge images. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29679050), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-308_rabbit-polyclonal-gfp-antibody-310202416173520.jpg "Western Blot: GFP Antibody [NB600-308] - MISP interacts & co-localizes with IQGAP1. (a) HEK293T cells transiently overexpressing GFP-IQGAP1 & FLAG-MISP were used for co-immunoprecipitation experiments from both sides (GFP trap or FLAG M2 beads). (b) Endogenous immunoprecipitation using IQGAP1 antibody or control immunoglobulin (IgG) was carried out in HeLa cell lysates & MISP was detected in the eluate. (c) In vitro interaction between purified MBP-MISP & GST-IQGAP1 was detected by GST & MBP pull-down experiments. (d) Immunoprecipiation experiments showing that GFP-MISP binds endogenous IQGAP1 (upper panel) & MISP binds to IQGAP1 endogenously using specific antibodies (lower panel) in mitotically arrested (taxol & nocodazole, respectively) HeLa cell lysates. (a–d) Images were gained from the same Western blot membrane after cutting/cropping & presented with different exposure times or contrast enhancement for better presentation purposes. The dividing lane marks the grouping of images of the same (or different) membrane. Uncropped blots, where applicable, are included in Supplementary Fig. 4. (e) HeLa cells were immunostained for MISP & IQGAP1 & co-localization was visualized in mitosis in single-plane confocal images, scale bar: 5 μm. Lower pictures: After manual thresholding white spots mark the co-localizing areas in the merge images. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29679050), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB600-308] - BRC-1 & BRD-1 are inter-dependent for localization.A) Co-localization between BRD-1::GFP (green) & TagRFP-T::BRC-1 (red) at late pachytene in live worms. Scale bar = 10 μm. B) Stills of germline nuclei from live worms expressing GFP::BRC-1 & mCherry::Histone H2B (mCherry::his-58; gfp::brc-1); merge & GFP channel; top two panels, respectively. GFP::BRC-1 expression in brd-1(ok1623) mutant at indicated meiotic stages. Bottom two panels show BRD-1::GFP localization in wild type & the brc-1(xoe4) mutant. Images are projections through half of the gonad. TZ = transition zone; EP = early pachytene; MP = mid pachytene; LP = late pachytene; DP = diplotene; DK = diakinesis. Scale bar = 5 μm. C) Immunoblot of whole worm extracts from indicated worms probed with anti-GFP & alpha -tubulin antibodies. Lane 1 = N2: wild type; Lane 2 = JEL515: gfp::brc-1; Lane 3 = JEL520: gfp::brc-1 brd-1(ok1623); Lane 4 = JEL744: brc-1(xoe4) brd-1::gfp; Lane 5 = JEL657: brd-1::gfp; Lane 6 = JEL678: brc-1(tm1145) brd-1::gfp. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30383767), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-308_rabbit-polyclonal-gfp-antibody-310202416165637.jpg "Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB600-308] - BRC-1 & BRD-1 are inter-dependent for localization.A) Co-localization between BRD-1::GFP (green) & TagRFP-T::BRC-1 (red) at late pachytene in live worms. Scale bar = 10 μm. B) Stills of germline nuclei from live worms expressing GFP::BRC-1 & mCherry::Histone H2B (mCherry::his-58; gfp::brc-1); merge & GFP channel; top two panels, respectively. GFP::BRC-1 expression in brd-1(ok1623) mutant at indicated meiotic stages. Bottom two panels show BRD-1::GFP localization in wild type & the brc-1(xoe4) mutant. Images are projections through half of the gonad. TZ = transition zone; EP = early pachytene; MP = mid pachytene; LP = late pachytene; DP = diplotene; DK = diakinesis. Scale bar = 5 μm. C) Immunoblot of whole worm extracts from indicated worms probed with anti-GFP & alpha -tubulin antibodies. Lane 1 = N2: wild type; Lane 2 = JEL515: gfp::brc-1; Lane 3 = JEL520: gfp::brc-1 brd-1(ok1623); Lane 4 = JEL744: brc-1(xoe4) brd-1::gfp; Lane 5 = JEL657: brd-1::gfp; Lane 6 = JEL678: brc-1(tm1145) brd-1::gfp. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30383767), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: GFP Antibody [NB600-308] - BRC-1 & BRD-1 are inter-dependent for localization.A) Co-localization between BRD-1::GFP (green) & TagRFP-T::BRC-1 (red) at late pachytene in live worms. Scale bar = 10 μm. B) Stills of germline nuclei from live worms expressing GFP::BRC-1 & mCherry::Histone H2B (mCherry::his-58; gfp::brc-1); merge & GFP channel; top two panels, respectively. GFP::BRC-1 expression in brd-1(ok1623) mutant at indicated meiotic stages. Bottom two panels show BRD-1::GFP localization in wild type & the brc-1(xoe4) mutant. Images are projections through half of the gonad. TZ = transition zone; EP = early pachytene; MP = mid pachytene; LP = late pachytene; DP = diplotene; DK = diakinesis. Scale bar = 5 μm. C) Immunoblot of whole worm extracts from indicated worms probed with anti-GFP & alpha -tubulin antibodies. Lane 1 = N2: wild type; Lane 2 = JEL515: gfp::brc-1; Lane 3 = JEL520: gfp::brc-1 brd-1(ok1623); Lane 4 = JEL744: brc-1(xoe4) brd-1::gfp; Lane 5 = JEL657: brd-1::gfp; Lane 6 = JEL678: brc-1(tm1145) brd-1::gfp. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30383767), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-308_rabbit-polyclonal-gfp-antibody-31020241616050.jpg "Western Blot: GFP Antibody [NB600-308] - BRC-1 & BRD-1 are inter-dependent for localization.A) Co-localization between BRD-1::GFP (green) & TagRFP-T::BRC-1 (red) at late pachytene in live worms. Scale bar = 10 μm. B) Stills of germline nuclei from live worms expressing GFP::BRC-1 & mCherry::Histone H2B (mCherry::his-58; gfp::brc-1); merge & GFP channel; top two panels, respectively. GFP::BRC-1 expression in brd-1(ok1623) mutant at indicated meiotic stages. Bottom two panels show BRD-1::GFP localization in wild type & the brc-1(xoe4) mutant. Images are projections through half of the gonad. TZ = transition zone; EP = early pachytene; MP = mid pachytene; LP = late pachytene; DP = diplotene; DK = diakinesis. Scale bar = 5 μm. C) Immunoblot of whole worm extracts from indicated worms probed with anti-GFP & alpha -tubulin antibodies. Lane 1 = N2: wild type; Lane 2 = JEL515: gfp::brc-1; Lane 3 = JEL520: gfp::brc-1 brd-1(ok1623); Lane 4 = JEL744: brc-1(xoe4) brd-1::gfp; Lane 5 = JEL657: brd-1::gfp; Lane 6 = JEL678: brc-1(tm1145) brd-1::gfp. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30383767), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB600-308] - Other IIS components are not altered by bsk RNAi.(A) Confocal images for germaria expressing Chico-GFP, myc-Dp110 or PDK1-GFP in the background of ctrl or bskRNAi from ovaries stained with anti-GFP or anti-myc. Scale bars, 10 μm. (B) Ovaries expressing LacZ driven by the puc promoter stained with anti-beta -galactosidase, anti-1B1, & DAPI. Note that JNK activity is moderately induced in late germarium & decreased in the following stages, while JNK activity is much stronger in follicle cells of a maturing egg. Scale bars, 20 μm. (C) Germaria from ovaries of ctrl & bskRNAi driven by nos-Gal4 stained with anti-AKT & anti-1B1. Scale bars, 10 μm. (D) Quantification of AKT intensity from cysts in germarium region 2A & 2B of ovaries with ctrl & bskRNAi driven by nos-Gal4. Intensities are normalized to the value of ctrl at region 2B. n = 7 & 8 germaria for ctrl & bskRNAi, respectively. Error bars represent SEM. *p<0.005. (E) Visualization of the InR mRNA by FISH in germaria from ovaries of ctrl & bskRNAi driven by nos-Gal4. Note that InR mRNA level is decreased by bsk RNAi. Germaria are outlined with dotted lines. Scale bars, 10 μm. (F) Quantification of InR mRNA density in region 2A & 2B germ cells from ovaries with indicated genotypes. n = 9 germaria for each genotype. Error bars represent SEM. *p<0.005. (G) Quantification of relative Myc-GFP intensity in region 2B cysts of ovaries illustrated in Figure 5H. n = 10 germaria for each genotype. Error bars represent SEM. *p<0.005.10.7554/eLife.49309.017Figure 5—figure supplement 1—source data 1.Relative AKT intensity, InR mRNA density, & Myc-GFP intensity in the germarium.Relative AKT intensity, InR mRNA density, & Myc-GFP intensity in the germarium. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31612862), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-308_rabbit-polyclonal-gfp-antibody-31020241616189.jpg "Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB600-308] - Other IIS components are not altered by bsk RNAi.(A) Confocal images for germaria expressing Chico-GFP, myc-Dp110 or PDK1-GFP in the background of ctrl or bskRNAi from ovaries stained with anti-GFP or anti-myc. Scale bars, 10 μm. (B) Ovaries expressing LacZ driven by the puc promoter stained with anti-beta -galactosidase, anti-1B1, & DAPI. Note that JNK activity is moderately induced in late germarium & decreased in the following stages, while JNK activity is much stronger in follicle cells of a maturing egg. Scale bars, 20 μm. (C) Germaria from ovaries of ctrl & bskRNAi driven by nos-Gal4 stained with anti-AKT & anti-1B1. Scale bars, 10 μm. (D) Quantification of AKT intensity from cysts in germarium region 2A & 2B of ovaries with ctrl & bskRNAi driven by nos-Gal4. Intensities are normalized to the value of ctrl at region 2B. n = 7 & 8 germaria for ctrl & bskRNAi, respectively. Error bars represent SEM. *p<0.005. (E) Visualization of the InR mRNA by FISH in germaria from ovaries of ctrl & bskRNAi driven by nos-Gal4. Note that InR mRNA level is decreased by bsk RNAi. Germaria are outlined with dotted lines. Scale bars, 10 μm. (F) Quantification of InR mRNA density in region 2A & 2B germ cells from ovaries with indicated genotypes. n = 9 germaria for each genotype. Error bars represent SEM. *p<0.005. (G) Quantification of relative Myc-GFP intensity in region 2B cysts of ovaries illustrated in Figure 5H. n = 10 germaria for each genotype. Error bars represent SEM. *p<0.005.10.7554/eLife.49309.017Figure 5—figure supplement 1—source data 1.Relative AKT intensity, InR mRNA density, & Myc-GFP intensity in the germarium.Relative AKT intensity, InR mRNA density, & Myc-GFP intensity in the germarium. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31612862), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.")
![Western Blot: GFP Antibody [NB600-308] - GFP Expression within A. thaliana upon infiltration with SWNT-PM-CytKH9/pDNA complexes.a Reporter construct design of transient GFP expression for the nucleus & mitochondria. b Representative confocal laser scanning microscopy images of A. thaliana root cells from a minimum of five seedlings for each condition (n = 5) 18 h post infiltration with SWNT-PM-CytKH9/pDNA complexes containing pDONR-35S-GFP or pDONR-Cox2-GFP reporter constructs for nuclear or mitochondrial expression, respectively. Mitochondria were stained with MitoTracker Red (CMXRos), & colocalization analysis was performed on GFP expression & MitoTracker signals. Scale bars represent 20 μm. c, d Representative western blots & their quantification from cytosolic (c) & mitochondrial (d) fractions isolated from approximately 60 seedlings 18 h post infiltration with SWNT NCs probed for GFP expression. Actin & cytochrome c were used as loading controls for cytosolic & mitochondrial proteins, respectively. Data points from five biological replicates (n = 5) are represented as the mean ± standard deviation. Statistical significance was determined by Brown-Forsythe & Welch one-way ANOVA test. For the cytosolic samples, P-values are 0.0027, & 0.0020 between DNA only & SWNT-PM-CytKH9, SWNT-PM-Cytcox, respectively, & 0.0253 between SWNT-PM-CytKH9 & SWNT-PM-Cytcox. For the mitochondrial samples, P-values are 0.0019, & 0.0333 between DNA only & SWNT-PM-CytKH9, & SWNT-PM-Cytcox, respectively, & 0.0359 between SWNT-PM-CytKH9 & SWNT-PM-Cytcox. ns – not statistically significant, *P < 0.05, **P < 0.01. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35577779), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-308_rabbit-polyclonal-gfp-antibody-310202416165624.jpg "Western Blot: GFP Antibody [NB600-308] - GFP Expression within A. thaliana upon infiltration with SWNT-PM-CytKH9/pDNA complexes.a Reporter construct design of transient GFP expression for the nucleus & mitochondria. b Representative confocal laser scanning microscopy images of A. thaliana root cells from a minimum of five seedlings for each condition (n = 5) 18 h post infiltration with SWNT-PM-CytKH9/pDNA complexes containing pDONR-35S-GFP or pDONR-Cox2-GFP reporter constructs for nuclear or mitochondrial expression, respectively. Mitochondria were stained with MitoTracker Red (CMXRos), & colocalization analysis was performed on GFP expression & MitoTracker signals. Scale bars represent 20 μm. c, d Representative western blots & their quantification from cytosolic (c) & mitochondrial (d) fractions isolated from approximately 60 seedlings 18 h post infiltration with SWNT NCs probed for GFP expression. Actin & cytochrome c were used as loading controls for cytosolic & mitochondrial proteins, respectively. Data points from five biological replicates (n = 5) are represented as the mean ± standard deviation. Statistical significance was determined by Brown-Forsythe & Welch one-way ANOVA test. For the cytosolic samples, P-values are 0.0027, & 0.0020 between DNA only & SWNT-PM-CytKH9, SWNT-PM-Cytcox, respectively, & 0.0253 between SWNT-PM-CytKH9 & SWNT-PM-Cytcox. For the mitochondrial samples, P-values are 0.0019, & 0.0333 between DNA only & SWNT-PM-CytKH9, & SWNT-PM-Cytcox, respectively, & 0.0359 between SWNT-PM-CytKH9 & SWNT-PM-Cytcox. ns – not statistically significant, *P < 0.05, **P < 0.01. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35577779), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: GFP Antibody [NB600-308] - VGLL1 regulates MMP9 transcription in gastric cancer cells. (a,b) Viability (a) & invasion (b) assay of NUGC3 cells. Cells were treated with 20 nM siScramble (SC) or siMMP9 for 48 h, & then stained with sulforhodamine B. Data are presented as mean ±SD. n = 3; * p < 0.05 (Student’s t-test). (c) MMP9 mRNA expression regulation by VGLL1 in gastric cancer cells treated with siVGLL1 or siSC (control) was measured by qPCR. (d) Changes in MMP9 expression upon knockdown or overexpression of VGLL1 assayed by western blotting in NUGC3 cells. (e) IHC of VGLL1 & MMP9 expression in the liver & lungs of an in vivo metastasis mouse model using surgical resection of tumors. Scale bar, 200 µm. (f) Construction of various luciferase reporter systems under control of the MMP9 promoter. (g) MMP9 promoter activities of the reporter systems containing modified TEA-binding sites were measured in NUGC3 cells. n = 3; * p < 0.05 (Student’s t-test). (h) Effect of TEAD4 on VGLL1-regulated MMP9 transcriptional activity. NUGC3 cells treated with siSC or siTEAD4 for 24 h were transfected with MMP9-luc, Renilla-luc, pCDNA3.1, & pcDNA3.1-myc-VGLL1 vectors for 48 h. n = 3; * p < 0.05 (Student’s t-test). (i) Interaction between VGLL1 & TEAD4. Lysates of NUGC3 cells that were transfected with pcDNA3.1-myc-VGLL1 & pEGFP-N1-TEAD4 were immunoprecipitated using anti-IgG, anti-GFP, & anti-Myc antibodies. Protein expression was analyzed by immunoblotting. (j) ChIP assays while using nuclear extracts of NUGC3 cells treated with siTEAD4. The ChIP-enriched DNA was subjected to PCR. (k) Target genes of VGLL1 & YAP. NUGC3 cells were transfected with pcDNA3, pcDNA3-myc-VGLL1, or pcDNA3-myc-YAP, & the mRNA expression levels were analyzed by RT-PCR. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31816819), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-308_rabbit-polyclonal-gfp-antibody-31020241616054.jpg "Western Blot: GFP Antibody [NB600-308] - VGLL1 regulates MMP9 transcription in gastric cancer cells. (a,b) Viability (a) & invasion (b) assay of NUGC3 cells. Cells were treated with 20 nM siScramble (SC) or siMMP9 for 48 h, & then stained with sulforhodamine B. Data are presented as mean ±SD. n = 3; * p < 0.05 (Student’s t-test). (c) MMP9 mRNA expression regulation by VGLL1 in gastric cancer cells treated with siVGLL1 or siSC (control) was measured by qPCR. (d) Changes in MMP9 expression upon knockdown or overexpression of VGLL1 assayed by western blotting in NUGC3 cells. (e) IHC of VGLL1 & MMP9 expression in the liver & lungs of an in vivo metastasis mouse model using surgical resection of tumors. Scale bar, 200 µm. (f) Construction of various luciferase reporter systems under control of the MMP9 promoter. (g) MMP9 promoter activities of the reporter systems containing modified TEA-binding sites were measured in NUGC3 cells. n = 3; * p < 0.05 (Student’s t-test). (h) Effect of TEAD4 on VGLL1-regulated MMP9 transcriptional activity. NUGC3 cells treated with siSC or siTEAD4 for 24 h were transfected with MMP9-luc, Renilla-luc, pCDNA3.1, & pcDNA3.1-myc-VGLL1 vectors for 48 h. n = 3; * p < 0.05 (Student’s t-test). (i) Interaction between VGLL1 & TEAD4. Lysates of NUGC3 cells that were transfected with pcDNA3.1-myc-VGLL1 & pEGFP-N1-TEAD4 were immunoprecipitated using anti-IgG, anti-GFP, & anti-Myc antibodies. Protein expression was analyzed by immunoblotting. (j) ChIP assays while using nuclear extracts of NUGC3 cells treated with siTEAD4. The ChIP-enriched DNA was subjected to PCR. (k) Target genes of VGLL1 & YAP. NUGC3 cells were transfected with pcDNA3, pcDNA3-myc-VGLL1, or pcDNA3-myc-YAP, & the mRNA expression levels were analyzed by RT-PCR. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31816819), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
