![Western Blot: GFP Antibody [FITC] [NB100-1183] - Lane 1: GFP. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Fluorescein Conjugated Anti-GFP at 1:1000 for 60 min at RT. Block: 1% BSA-TTBS for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa.](http://images.novusbio.com/fullsize/GFP-Antibody-[FITC]-Western-Blot-NB100-1183-img0006.jpg "Western Blot: GFP Antibody [FITC] [NB100-1183] - Lane 1: GFP. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Fluorescein Conjugated Anti-GFP at 1:1000 for 60 min at RT. Block: 1% BSA-TTBS for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa.")
| Reactivity | All-NASpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | FITC |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NB100-1365 | |||
| FITC | NB100-1183-0.025mg | |||
| Unconjugated | NB600-303 | |||
| Description | For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room GFP Antibody Fluorescein Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Fluorescein and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum |
| Immunogen | The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This product has been tested by ELISA and SDS-Page and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% Sodium Azide |
| Purity | Immunogen affinity purified |
| Publication using NB100-1183 | Applications | Species |
|---|---|---|
| Olszak T, Neves JF, Dowds CM et al. Protective mucosal immunity mediated by epithelial CD1d and IL-10 Nature 2014-05-22 [PMID: 24717441] (IHC-Fr) Details: This citation used the FITC version of this antibody. |
IHC-Fr |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
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reviewed by:
Verified Customer |
ICC | Other | 03/22/2010 |
Summary
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Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: GFP Antibody [FITC] [NB100-1183] - Staining of mouse spleen cells Tissue: spleen cells infected with MHV68-H2bYFP. As seen in: Collins CM, Speck SH (2012) Tracking Murine Gammaherpesvirus 68 Infection of Germinal Center B Cells.](http://images.novusbio.com/fullsize/GFP-Antibody-[FITC]-Immunocytochemistry-Immunofluorescence-NB100-1183-img0004.jpg "Immunocytochemistry/Immunofluorescence: GFP Antibody [FITC] [NB100-1183] - Staining of mouse spleen cells Tissue: spleen cells infected with MHV68-H2bYFP. As seen in: Collins CM, Speck SH (2012) Tracking Murine Gammaherpesvirus 68 Infection of Germinal Center B Cells.")
![Flow Cytometry: Rabbit IgG Isotype Control [FITC] [NBP2-24892] - Analysis of TLR7 in Ramos cells using 2 ug of NBP2-24892. Shaded histogram represents FITC-conjugated rabbit IgG isotype control ; red represents anti-TLR7 antibody.](https://images.novusbio.com/images/Rabbit-IgG-Isotype-Control-[FITC]-Flow-Cytometry-NBP2-24892-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [FITC] [NBP2-24892] - Analysis of TLR7 in Ramos cells using 2 ug of NBP2-24892. Shaded histogram represents FITC-conjugated rabbit IgG isotype control ; red represents anti-TLR7 antibody.")