![Western Blot: FRG1 Antibody [NBP2-99204] - Lane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:29 kDaObserved band size:29 kDa](http://images.novusbio.com/fullsize/FRG1-Antibody-Western-Blot-NBP2-99204-img0002.jpg "Western Blot: FRG1 Antibody [NBP2-99204] - Lane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:29 kDaObserved band size:29 kDa")
| Reactivity | Mu, HuSpecies Glossary |
| Applications | WB, ICC/IF, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles. |
| Immunogen | Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Mouse FRG1. |
| Predicted Species | Human (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | FRG1 |
| Purity | Antigen and protein A Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.2 um filtered solution in PBS |
| Preservative | No Preservative |
| Purity | Antigen and protein A Affinity-purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | FRG1 |
![Immunocytochemistry/Immunofluorescence: FRG1 Antibody [NBP2-99204] - Immunofluorescence staining of FRG1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human FRG1 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green).Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/FRG1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-99204-img0001.jpg "Immunocytochemistry/Immunofluorescence: FRG1 Antibody [NBP2-99204] - Immunofluorescence staining of FRG1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human FRG1 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green).Positive staining was localized to nucleus.")
![Immunoprecipitation: FRG1 Antibody [NBP2-99204] - Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg A549 Whole Cell Lysate2 uL anti-Frg1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Frg1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 28 kDaObserved band size: 38 kDa](http://images.novusbio.com/fullsize/FRG1-Antibody-Immunoprecipitation-NBP2-99204-img0003.jpg "Immunoprecipitation: FRG1 Antibody [NBP2-99204] - Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg A549 Whole Cell Lysate2 uL anti-Frg1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Frg1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 28 kDaObserved band size: 38 kDa")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")