Exosome Detection (Simple Western) Antibody Pack Summary
| Description |
This pack contains 1 vial each of: MAB50482-SP (25 ug); NBP2-67884 (100 uL); MAB1663-SP (25 ug); NB100-1965SS (0.025 ml) |
| Immunogen |
See individual datasheets |
| Specificity |
Please see each vial's data sheet for specificity information specific to each product. |
Applications/Dilutions
| Dilutions |
- Flow Cytometry
- Immunohistochemistry
- Simple Western
- Western Blot
|
| Application Notes |
See individual datasheets of components for their validated applications |
Reactivity Notes
See individual datasheets of components for their validated species
Packaging, Storage & Formulations
| Storage |
Storage is content dependent. |
| Buffer |
See individual datasheets |
Kit Components
Background
The Exosome Detection (Simple Western) Antibody Pack contains antibodies from each category recommended by the International Society for Extracellular Vesicles (ISEV) for characterization of extracellular vesicles (EVs). It includes one tetraspanin (CD63), one intracellular protein associated with multivesicular body (MVB) formation (TSG101), and one chaperone protein (HSP70). The pack also contains an antibody to determine the degree of contamination of exosome prep with secretory vesicles (Calnexin). All antibodies have been validation for use in Simple Western.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are
guaranteed for 1 year from date of receipt.
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Product General Protocols
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Video Protocols
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Additional Exosome Detection (Simple Western) Products
Blogs on Exosome Detection (Simple Western)
![Simple Western: Exosome Detection (Simple Western) Antibody Pack [NBP3-11742] - Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) by heat shocked (HS), loaded at 0.2 mg/mL. A specific band was detected for HSP70/HSPA1A at approximately 67 kDa (as indicated) using 0.5 ug/mL of Mouse Anti-Human/Mouse/Rat HSP70/HSPA1A Monoclonal Antibody (Catalog # MAB1663). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Exosome-Detection-Simple-Western-Antibody-Pack-Simple-Western-NBP3-11742-img0001.jpg "Simple Western: Exosome Detection (Simple Western) Antibody Pack [NBP3-11742] - Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) by heat shocked (HS), loaded at 0.2 mg/mL. A specific band was detected for HSP70/HSPA1A at approximately 67 kDa (as indicated) using 0.5 ug/mL of Mouse Anti-Human/Mouse/Rat HSP70/HSPA1A Monoclonal Antibody (Catalog # MAB1663). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.")
![Simple Western: Exosome Detection (Simple Western) Antibody Pack [NBP3-11742] - Simple Western lane view shows lysates of human platelets, loaded at 0.2 mg/mL. A specific band was detected for CD63 at approximately 59 kDa (as indicated) using 20 ug/mL of Rabbit Anti-Human CD63 Monoclonal Antibody (Catalog # MAB50482). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Exosome-Detection-Simple-Western-Antibody-Pack-Simple-Western-NBP3-11742-img0002.jpg "Simple Western: Exosome Detection (Simple Western) Antibody Pack [NBP3-11742] - Simple Western lane view shows lysates of human platelets, loaded at 0.2 mg/mL. A specific band was detected for CD63 at approximately 59 kDa (as indicated) using 20 ug/mL of Rabbit Anti-Human CD63 Monoclonal Antibody (Catalog # MAB50482). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.")
