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ERK1 Antibody (OTI4D7) - Azide and BSA Free

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Western Blot: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERK1.
Western Blot: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERK1.
Immunocytochemistry/ Immunofluorescence: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY ERK1.
Immunohistochemistry: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of Human tonsil tissue.
Western Blot: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of extracts (35ug) from 9 different cell lines. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: ...read more
Immunohistochemistry: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of Adenocarcinoma of Human endometrium tissue.
Immunohistochemistry: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of Adenocarcinoma of Human ovary tissue.
Immunohistochemistry: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of Human lymphoma tissue.
Immunohistochemistry: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of Human pancreas tissue.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI4D7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-71239AF350
Alexa Fluor 405
NBP2-71239AF405
Alexa Fluor 488
NBP2-71239AF488
Alexa Fluor 532
NBP2-71239AF532
Alexa Fluor 594
NBP2-71239AF594
Alexa Fluor 647
NBP2-71239AF647
Alexa Fluor 700
NBP2-71239AF700
Alexa Fluor 750
NBP2-71239AF750
Allophycocyanin
NBP2-71239APC
Biotin
NBP2-71239B
CoraFluor 1
NBP2-71239CL1
DyLight 350
NBP2-71239UV
DyLight 405
NBP2-71239V
DyLight 488
NBP2-71239G
DyLight 550
NBP2-71239R
DyLight 594
NBP2-71239DL594
DyLight 650
NBP2-71239C
DyLight 680
NBP2-71239FR
DyLight 755
NBP2-71239IR
FITC
NBP2-71239F
HRP
NBP2-71239H
Janelia Fluor 525
NBP2-71239JF525
Janelia Fluor 549
NBP2-71239JF549
Janelia Fluor 646
NBP2-71239JF646
Janelia Fluor 669
NBP2-71239JF669
PE
NBP2-71239PE
PerCP
NBP2-71239PCP
Unconjugated
NBP2-45814
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-45814
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ERK1 Antibody (OTI4D7) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human MAPK3(NP_002737) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MAPK3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:200-2000
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for ERK1 Antibody (OTI4D7) - Azide and BSA Free

  • EC 2.7.11
  • ERK1
  • ERK-1
  • ERK1p44-MAPK
  • ERT2
  • Extracellular signal-regulated kinase 1
  • extracellular signal-related kinase 1
  • HS44KDAP
  • HUMKER1A
  • Insulin-stimulated MAP2 kinase
  • MAP kinase 1
  • MAP kinase 3
  • MAP kinase isoform p44
  • MAPK 1
  • MAPK 3
  • MAPK3
  • MGC20180
  • Microtubule-associated protein 2 kinase
  • Mitogen-activated protein kinase 1
  • mitogen-activated protein kinase 3
  • P44ERK1
  • p44-ERK1
  • p44mapk
  • PRKM3
  • PRKM3EC 2.7.11.24

Background

p44 MAP Kinase (Erk1) is a member of a family of mitogen-activated protein kinases that participate in diverse cellular functions, including the regulation of cell growth and differentiation (1,2). Erk1 regulates meiosis and mitosis by phosphorylating a number of transcription factors such as ELK-1. Activation of Erk1 occurs through phosphorylation of threonine 202 and tyrosine 204 (of human MAP kinase) (3). Erk1 is also known to autophosphorylate on tyrosine (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MAPK3