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ER beta/NR3A2 Antibody (6B12)

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Western Blot: ER beta/NR3A2 Antibody (6B12) [NB200-304] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Estrogen Receptor beta ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
6B12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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ER beta/NR3A2 Antibody (6B12) Summary

Immunogen
Amino acids 1-153 of human ER beta/NR3A2 expressed in E. coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ESR2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10 - 1:500
  • Western Blot 1:500-1:3000
Application Notes
IHC usage reported in scientific literature.
Theoretical MW
59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB200-304 in the following applications:

  • 5 publications
  • WB
    5 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for ER beta/NR3A2 Antibody (6B12)

  • ER beta
  • Erb
  • ER-beta
  • ESR2
  • ESR-BETA
  • ESTRB
  • estrogen receptor 2 (ER beta)
  • estrogen receptor beta 4
  • estrogen receptor beta
  • NR3A2
  • NR3A2ESRB
  • Nuclear receptor subfamily 3 group A member 2

Background

The human ER-iis a newly discovered estrogen receptor initially cloned and characterized from testis. The size and structure of ER-iis very similar to ER-a with the ligand and DNA binding domains being highly conserved, while the amino terminus which serves as their transactivation domains have diverged significantly. Similar in function to ER-a, ER-ibinds to estrogen with a high affinity and regulates estrogen specific gene activation through direct interaction with estrogen response elements (ERE's).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ER beta/NR3A2 Antibody (NB200-304)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IF/IHC, WB.


Filter By Application
IF/IHC
(5)
WB
(5)
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Filter By Species
Human
(5)
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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ESR2
Entrez
Uniprot