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DNA Polymerase beta Antibody

Images

 
Western Blot: DNA Polymerase beta Antibody [H00005423-B01P] - Analysis of POLB expression in transfected 293T cell line by POLB polyclonal antibody. Lane 1: POLB transfected lysate(36.85 KDa). Lane 2: Non-transfected ...read more
Immunocytochemistry/ Immunofluorescence: DNA Polymerase beta Antibody [H00005423-B01P] - Analysis of purified antibody to POLB on HeLa cell. (antibody concentration 10 ug/ml)
Immunohistochemistry-Paraffin: DNA Polymerase beta Antibody [H00005423-B01P] - Analysis of purified antibody to POLB on formalin-fixed paraffin-embedded human salivary gland. (antibody concentration 3 ug/ml)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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DNA Polymerase beta Antibody Summary

Description
Quality control test: Antibody reactive against mammalian transfected lysate.
Immunogen
POLB (AAI06910.1, 1 a.a. - 335 a.a.) full-length human protein. MSKRKAPQETLNGGITDMLTELANFEKNVSQAIHKYNAYRKAASVIAKYPHKIKSGAEAKKLPGVGTKIAEKIDEFLATGKLRKLEKIRQDDTSSSINFLTRVSGIGPSAARKFVDEGIKTLEDLRKNEDKLNHHQRIGLKYFGDFEKRIPREEMLQMQDIVLNEVKKVDSEYIATVCGSFRRGAESSGDMDVLLTHPSFTSESTKQPKLLHQVVEQLQKVHFITDTLSKGETKFMGVCQLPSKNDEKEYPHRRIDIRLIPKDQYYCGVLYFTGSDIFNKNMRAHALEKGFTINEYTIRPLGVTGVAGEPLPVDSEKDIFDYIQWKYREPKDRSE
Specificity
polymerase (DNA directed), beta
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
POLB
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Antibody reactivity against mammalian transfected lysate in WB, IHC-P, ELISA and IF.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DNA Polymerase beta Antibody

  • DNA pol beta
  • DNA polymerase beta subunit
  • DNA Polymerase beta
  • EC 2.7.7.7
  • EC 4.2.99.-
  • MGC125976
  • POLB
  • polymerase (DNA directed), beta

Background

In eukaryotic cells, DNA polymerase beta (POLB) performs base excision repair (BER) required for DNA maintenance, replication, recombination, and drug resistance. Also see POLA (MIM 312040).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DNA Polymerase beta Antibody (H00005423-B01P) (0)

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Secondary Antibodies

 

Isotype Controls

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PARP Antibody Assays aid both Apoptosis and Cancer Research
The PARP (Poly(ADP-ribose) polymerase) protein is a zinc-dependant nuclear enzyme whose main role is to detect and repair DNA single-strand breaks (SSB). However, PARP antibody research has revealed there are at least 17 PARP proteins, which also play...  Read full blog post.

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Bioinformatics

Gene Symbol POLB
Entrez
Uniprot