Coagulation Factor X Antibody

Images

 
Image 1 of 1
(
Enlarge)
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human Coagulation Factor X Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

  • Loading...
View Available Formulations
Catalog# & Formulation Size Price
Datasheet
Reviews & Publications
Protocols & FAQs
Support & Research

Coagulation Factor X Antibody Summary

Immunogen
S. frugiperda insect cell line Sf 21-derived recombinant human Coagulation Factor X
Leu24-Lys488
Accession # P00742
Specificity
Detects human Coagulation Factor X in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
F10
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 25 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publication using
AF1063 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Coagulation Factor X Antibody

  • Cf10
  • Coagulation Factor X
  • EC 3.4.21
  • EC 3.4.21.6
  • F10
  • factor Xa
  • FX
  • FXa
  • Prothrombinase
  • Stuart Factor
  • Stuart-Prower factor

Background

Factor X (Coagulation factor X; also Stuart factor) is a 74-76 kDa glycoprotein member of the peptidase S1 family of molecules. It is secreted by hepatocytes, and plays a key role in the coagulation cascade. Normally, Factor X circulates as a zymogen (or inactive form). Upon disruption of the vasculature, Factor X, and the circulating zymogen Factor V interact, and form what's called the prothrombinase complex on negatively-charged membrane phospholipids of platelets and endothelial cells. This complex converts prothrombin (Factor II) into thrombin, and thus initiates clot formation. Factor X (as a zymogen) is a disulfide-linked heterodimer. Its two chains are the result of intracellular processing of a 74-76 kDa single chain precursor. This creates a 55-57 kDa C-terminal heavy chain, and a 17-18 kDa N-terminal light chain. Prothrombinase complex formation results in the cleavage of the heavy chain, leading to the generation of a 45-46 kDa, prothrombin-cleaving active chain, and a soluble 10 kDa activation fragment. Cleavage is not the result of Factor V activity, but that of either Tissue Factor or Factor IXa, and the activities of there two enzymes are tightly regulated by the carbohydrates bound to the 10 kDa activation fragment. Mature human Factor X is synthesized as a 488 amino acid (aa) preproprecursor that contains a 31 aa signal sequence, a 9 aa prosegment (aa 32-40), a 139 aa light chain (aa 41-179), and a 306 aa heavy chain (aa 183-488). The light chain possesses a Gla domain that binds to Factor V (aa 41-85) plus two EGF-like motifs (aa 86-165), while the heavy chain contains the activation peptide sequence (aa 183-234) followed by a large peptidase S1 domain (aa 235-467). Over aa 24-488, human Factor X shares 77% aa sequence identity with mouse Factor X.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-58268
Species: Hu, Mu
Applications: IHC, IHC-P, WB
DCF300
Species: Hu
Applications: ELISA
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  PDF SDS

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Coagulation Factor X Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol F10
Uniprot