![Western Blot: CDS1 Antibody (2D10) [H00001040-M01] - CDS1 immunoreactivity and CDS activity localises to mitochondria in rat brain and liver. [A] Rat brain and [B] liver fractionated and analysed by CDS1 immunoreactivity by Western blot and for CDS activity. CDS activity was monitored in triplicate and error bars denote S.E.M. WCL, whole cell lysate; Micro, microsomes; C.Mito, crude mitochondria; Cyto, cytosol; P.Mito, pure mitochondria, MAMs, mitochondrial associated membranes; CDS, CDP-diacylglycerol synthase. Image collected and cropped by CiteAb from the following publication (www.linkinghub.elsevier.com/retrieve/pii/S1388198117302421) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CDS1-Antibody-2D10-Western-Blot-H00001040-M01-img0009.jpg "Western Blot: CDS1 Antibody (2D10) [H00001040-M01] - CDS1 immunoreactivity and CDS activity localises to mitochondria in rat brain and liver. [A] Rat brain and [B] liver fractionated and analysed by CDS1 immunoreactivity by Western blot and for CDS activity. CDS activity was monitored in triplicate and error bars denote S.E.M. WCL, whole cell lysate; Micro, microsomes; C.Mito, crude mitochondria; Cyto, cytosol; P.Mito, pure mitochondria, MAMs, mitochondrial associated membranes; CDS, CDP-diacylglycerol synthase. Image collected and cropped by CiteAb from the following publication (www.linkinghub.elsevier.com/retrieve/pii/S1388198117302421) licensed under a CC-BY license.")
| Reactivity | Hu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, KD |
| Clone | 2D10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | CDS1 (NP_001254, 1 a.a. ~ 98 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MLELRHRGSCPGPREAVSPPHREGEAAGGDHETESTSDKETDIDDRYGDLDSRTDSDIPEIPPSSDRTPEILKKALSGLSSRWKNWWIRGILTLTMIS |
| Specificity | CDS1 - CDP-diacylglycerol synthase (phosphatidate cytidylyltransferase) 1 (2D10) |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CDS1 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||
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reviewed by:
Bryan Tinsley |
WB | Rat | 09/08/2014 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for CDS1 Antibody (H00001040-M01)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: CDS1 Antibody (2D10) [H00001040-M01] - Analysis of monoclonal antibody to CDS1 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/CDS1-Antibody-2D10-Immunocytochemistry-Immunofluorescence-H00001040-M01-img0005.jpg "Immunocytochemistry/Immunofluorescence: CDS1 Antibody (2D10) [H00001040-M01] - Analysis of monoclonal antibody to CDS1 on HeLa cell . Antibody concentration 10 ug/ml.")
![Western Blot: CDS1 Antibody (2D10) [H00001040-M01] - CDS1 monoclonal antibody (M01), clone 2D10. Analysis of CDS1 expression in human liver.](http://images.novusbio.com/fullsize/CDS1-Antibody-2D10-Western-Blot-H00001040-M01-img0007.jpg "Western Blot: CDS1 Antibody (2D10) [H00001040-M01] - CDS1 monoclonal antibody (M01), clone 2D10. Analysis of CDS1 expression in human liver.")
![ELISA: CDS1 Antibody (2D10) [H00001040-M01] - Detection limit for recombinant GST tagged CDS1 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CDS1-Antibody-2D10-ELISA-H00001040-M01-img0008.jpg "ELISA: CDS1 Antibody (2D10) [H00001040-M01] - Detection limit for recombinant GST tagged CDS1 is 0.1 ng/ml as a capture antibody.")
![Western Blot: CDS1 Antibody (2D10) [H00001040-M01] - Knockdown of CDS1 with siRNA does not lead to a reduction in the p55 band. Mitochondrial fractions prepared from control, negative siRNA-treated and CDS1 siRNA-treated H9c2 cells were analysed for CDS1 immunoreactivity by Western blot. Image collected and cropped by CiteAb from the following publication (www.sciencedirect.com/science/article/pii/S1388198117302421?via%3Dihub) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CDS1-Antibody-2D10-Knockdown-Validated-H00001040-M01-img0010.jpg "Western Blot: CDS1 Antibody (2D10) [H00001040-M01] - Knockdown of CDS1 with siRNA does not lead to a reduction in the p55 band. Mitochondrial fractions prepared from control, negative siRNA-treated and CDS1 siRNA-treated H9c2 cells were analysed for CDS1 immunoreactivity by Western blot. Image collected and cropped by CiteAb from the following publication (www.sciencedirect.com/science/article/pii/S1388198117302421?via%3Dihub) licensed under a CC-BY license.")
![Western Blot: CDS1 Antibody (2D10) [H00001040-M01] - Localisation of CDS1 immunoreactivity & CDS activity to mitochondria.[A, B] Differentiated H9c2 cells fractionated & analysed for [A] CDS1 immunoreactivity by Western blot; [B] CDS activity. [C-E] Rat heart fractionated & analysed for [C] CDS1 immunoreactivity & markers, [D] CDS1 immunoreactivity (entire Western blot shown) & [E] CDS activity. [F–H] Rat heart fractionated in the presence of the protease, subtilisin & analysed for [F] CDS1 immunoreactivity by western blot, [G] CDS activity & [H] PI synthase (PIS) activity. CDS & PIS activity was monitored in triplicate & error bars denote ± S.E.M. COXIV, GRP75 & cyto c are markers for mitochondria, PITPNC1 & PITP alpha are cytosolic markers & calnexin is a marker for the ER. WCL, whole cell lysate; Micro, microsomes; C.Mito, crude mitochondria; Cyto, cytosol; P.Mito, pure mitochondria, MAMs, mitochondrial associated membranes; PIS, PI synthase; CDS, CDP-diacylglycerol synthase. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29253589), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00001040-m01_mouse-monoclonal-cds1-antibody-2d10-31020241548365.jpg "Western Blot: CDS1 Antibody (2D10) [H00001040-M01] - Localisation of CDS1 immunoreactivity & CDS activity to mitochondria.[A, B] Differentiated H9c2 cells fractionated & analysed for [A] CDS1 immunoreactivity by Western blot; [B] CDS activity. [C-E] Rat heart fractionated & analysed for [C] CDS1 immunoreactivity & markers, [D] CDS1 immunoreactivity (entire Western blot shown) & [E] CDS activity. [F–H] Rat heart fractionated in the presence of the protease, subtilisin & analysed for [F] CDS1 immunoreactivity by western blot, [G] CDS activity & [H] PI synthase (PIS) activity. CDS & PIS activity was monitored in triplicate & error bars denote ± S.E.M. COXIV, GRP75 & cyto c are markers for mitochondria, PITPNC1 & PITP alpha are cytosolic markers & calnexin is a marker for the ER. WCL, whole cell lysate; Micro, microsomes; C.Mito, crude mitochondria; Cyto, cytosol; P.Mito, pure mitochondria, MAMs, mitochondrial associated membranes; PIS, PI synthase; CDS, CDP-diacylglycerol synthase. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29253589), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: CDS1 Antibody (2D10) [H00001040-M01] - Localisation of CDS1 immunoreactivity & CDS activity to mitochondria.[A, B] Differentiated H9c2 cells fractionated & analysed for [A] CDS1 immunoreactivity by Western blot; [B] CDS activity. [C-E] Rat heart fractionated & analysed for [C] CDS1 immunoreactivity & markers, [D] CDS1 immunoreactivity (entire Western blot shown) & [E] CDS activity. [F–H] Rat heart fractionated in the presence of the protease, subtilisin & analysed for [F] CDS1 immunoreactivity by western blot, [G] CDS activity & [H] PI synthase (PIS) activity. CDS & PIS activity was monitored in triplicate & error bars denote ± S.E.M. COXIV, GRP75 & cyto c are markers for mitochondria, PITPNC1 & PITP alpha are cytosolic markers & calnexin is a marker for the ER. WCL, whole cell lysate; Micro, microsomes; C.Mito, crude mitochondria; Cyto, cytosol; P.Mito, pure mitochondria, MAMs, mitochondrial associated membranes; PIS, PI synthase; CDS, CDP-diacylglycerol synthase. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29253589), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00001040-m01_mouse-monoclonal-cds1-antibody-2d10-310202415483622.jpg "Western Blot: CDS1 Antibody (2D10) [H00001040-M01] - Localisation of CDS1 immunoreactivity & CDS activity to mitochondria.[A, B] Differentiated H9c2 cells fractionated & analysed for [A] CDS1 immunoreactivity by Western blot; [B] CDS activity. [C-E] Rat heart fractionated & analysed for [C] CDS1 immunoreactivity & markers, [D] CDS1 immunoreactivity (entire Western blot shown) & [E] CDS activity. [F–H] Rat heart fractionated in the presence of the protease, subtilisin & analysed for [F] CDS1 immunoreactivity by western blot, [G] CDS activity & [H] PI synthase (PIS) activity. CDS & PIS activity was monitored in triplicate & error bars denote ± S.E.M. COXIV, GRP75 & cyto c are markers for mitochondria, PITPNC1 & PITP alpha are cytosolic markers & calnexin is a marker for the ER. WCL, whole cell lysate; Micro, microsomes; C.Mito, crude mitochondria; Cyto, cytosol; P.Mito, pure mitochondria, MAMs, mitochondrial associated membranes; PIS, PI synthase; CDS, CDP-diacylglycerol synthase. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29253589), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: CDS1 Antibody (2D10) [H00001040-M01] - Localisation of CDS1 immunoreactivity & CDS activity to mitochondria.[A, B] Differentiated H9c2 cells fractionated & analysed for [A] CDS1 immunoreactivity by Western blot; [B] CDS activity. [C-E] Rat heart fractionated & analysed for [C] CDS1 immunoreactivity & markers, [D] CDS1 immunoreactivity (entire Western blot shown) & [E] CDS activity. [F–H] Rat heart fractionated in the presence of the protease, subtilisin & analysed for [F] CDS1 immunoreactivity by western blot, [G] CDS activity & [H] PI synthase (PIS) activity. CDS & PIS activity was monitored in triplicate & error bars denote ± S.E.M. COXIV, GRP75 & cyto c are markers for mitochondria, PITPNC1 & PITP alpha are cytosolic markers & calnexin is a marker for the ER. WCL, whole cell lysate; Micro, microsomes; C.Mito, crude mitochondria; Cyto, cytosol; P.Mito, pure mitochondria, MAMs, mitochondrial associated membranes; PIS, PI synthase; CDS, CDP-diacylglycerol synthase. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29253589), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00001040-m01_mouse-monoclonal-cds1-antibody-2d10-310202415482211.jpg "Western Blot: CDS1 Antibody (2D10) [H00001040-M01] - Localisation of CDS1 immunoreactivity & CDS activity to mitochondria.[A, B] Differentiated H9c2 cells fractionated & analysed for [A] CDS1 immunoreactivity by Western blot; [B] CDS activity. [C-E] Rat heart fractionated & analysed for [C] CDS1 immunoreactivity & markers, [D] CDS1 immunoreactivity (entire Western blot shown) & [E] CDS activity. [F–H] Rat heart fractionated in the presence of the protease, subtilisin & analysed for [F] CDS1 immunoreactivity by western blot, [G] CDS activity & [H] PI synthase (PIS) activity. CDS & PIS activity was monitored in triplicate & error bars denote ± S.E.M. COXIV, GRP75 & cyto c are markers for mitochondria, PITPNC1 & PITP alpha are cytosolic markers & calnexin is a marker for the ER. WCL, whole cell lysate; Micro, microsomes; C.Mito, crude mitochondria; Cyto, cytosol; P.Mito, pure mitochondria, MAMs, mitochondrial associated membranes; PIS, PI synthase; CDS, CDP-diacylglycerol synthase. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29253589), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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