![Western Blot: CDK1/2/3 [p Thr14] Antibody (4I9L3) [NBP3-33561] - Western blot analysis of various lysates using CDK1/2/3 Rabbit mAb at 1:1000 dilution. Both NIH/3T3 cells and C6 cells were treated by Hydroxyurea (4 Mm) at 37C for 20 hours or treated by Nocodazole (100 ng/mL) at 37C for 24 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-33561_rabbit-cdk1-2-3-p-thr14-mab-4i9l3-1012202411545528.jpg "Western Blot: CDK1/2/3 [p Thr14] Antibody (4I9L3) [NBP3-33561] - Western blot analysis of various lysates using CDK1/2/3 Rabbit mAb at 1:1000 dilution. Both NIH/3T3 cells and C6 cells were treated by Hydroxyurea (4 Mm) at 37C for 20 hours or treated by Nocodazole (100 ng/mL) at 37C for 24 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 30s.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA |
| Clone | 4I9L3 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | A synthetic phosphorylated peptide around T14 of human CDK1/2/3 (P06493). Sequence: EGTYG |
| Modification | p Thr14 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 34 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 50% glycerol, 0.05% BSA |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
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