Canine Calcitonin ELISA Kit (Colorimetric) Summary
Description |
Assay time: 2 hours |
Standard Curve Range |
12.35 - 1000 pg/mL |
Sensitivity |
5.28 pg/mL |
Assay Type |
Competitive ELISA |
Inter-Assay |
%CV < 12 |
Intra-Assay |
%CV < 10 |
Spike Recovery |
84 - 100% |
Sample Volume |
50 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
CALCA |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Assay Diluent A
- Assay Diluent B
- Detection Reagent A
- Detection Reagent B
- Instruction manual
- Plate sealer for 96 wells
- Pre-coated ready to use 96-well strip plate
- Standard Diluent
- Standard
- Stop Solution
- TMB Substrate
- Wash Buffer (30 x concentrate)
|
Alternate Names for Canine Calcitonin ELISA Kit (Colorimetric)
Background
Calcitonin is a 32 amino acid peptide hormone synthesized by the parafollicular cells of the thyroid. It causes a rapid, but short lived, reduction in serum calcium and phosphate by promoting the incorporation of those ions in the bones. This effect is opposite to that of parathyroid hormone. Staining for calcitonin may be used for the identification of a spectrum of C cell proliferative abnormalities ranging from C cell hyperplasia to invasive tumors. Staining for calcitonin in medullary carcinoma of the thyroid produces a fine granular pattern in the cytoplasm. Amyloid deposits within the tumor may also exhibit varying degrees of calcitonin activity.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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