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Calcitonin Antibody (CALCA/8639R)

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Formalin-fixed, paraffin-embedded human thyroid stained with Calcitonin antibody (CALCA/8639R). Inset: PBS instead of primary antibody; secondary only negative control.
Formalin-fixed, paraffin-embedded human thyroid stained with Calcitonin antibody (CALCA/8639R). Inset: PBS instead of primary antibody; secondary only negative control.
Formalin-fixed, paraffin-embedded human medullary carcinoma stained with Calcitonin antibody (CALCA/8639R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
CALCA/8639R
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-20778AF350
Alexa Fluor 405
NBP3-20778AF405
Alexa Fluor 488
NBP3-20778AF488
Alexa Fluor 532
NBP3-20778AF532
Alexa Fluor 594
NBP3-20778AF594
Alexa Fluor 647
NBP3-20778AF647
Alexa Fluor 700
NBP3-20778AF700
Alexa Fluor 750
NBP3-20778AF750
Allophycocyanin
NBP3-20778APC
Biotin
NBP3-20778B
CoraFluor 1
NBP3-20778CL1
DyLight 350
NBP3-20778UV
DyLight 405
NBP3-20778V
DyLight 488
NBP3-20778G
DyLight 550
NBP3-20778R
DyLight 594
NBP3-20778DL594
DyLight 650
NBP3-20778C
DyLight 680
NBP3-20778FR
DyLight 755
NBP3-20778IR
FITC
NBP3-20778F
HRP
NBP3-20778H
Janelia Fluor 525
NBP3-20778JF525
Janelia Fluor 549
NBP3-20778JF549
Janelia Fluor 585
NBP3-20778JF585
Janelia Fluor 635
NBP3-20778JF635
Janelia Fluor 646
NBP3-20778JF646
Janelia Fluor 669
NBP3-20778JF669
PE
NBP3-20778PE
PerCP
NBP3-20778PCP
Unconjugated
NBP3-20778
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP3-20778
Azide and BSA Free
NBP3-20778
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Calcitonin Antibody (CALCA/8639R) Summary

Description
Positive Controls: Human thyroid.

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant fragment of human Calcitonin protein (around aa 1-141) (exact sequence is proprietary)
Localization
Cytoplasm. Secreted
Isotype
IgG Kappa
Clonality
Monoclonal
Host
Rabbit
Gene
CALCA
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1:10-1:20
Application Notes
Immunohistochemistry (Formalin-fixed): 1:10-1:20 for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Calcitonin Antibody (CALCA/8639R)

  • ATP2B2plasma membrane calcium ATPase
  • ATPase, Ca++ transporting, plasma membrane 4
  • CALC1
  • CALCA
  • Calcitonin
  • DKFZp686G08106
  • DKFZp686M088
  • EC 3.6.3
  • EC 3.6.3.8
  • Katacalcin
  • Matrix-remodeling-associated protein 1
  • matrix-remodelling associated 1
  • MXRA1
  • PCT
  • Plasma membrane calcium ATPase isoform 4
  • Plasma membrane calcium pump isoform 4
  • plasma membrane calcium pump
  • plasma membrane calcium-transporting ATPase 4
  • PMCA4b
  • PMCA4PMCA4x
  • sarcolemmal calcium pump

Background

Calcitonin is a 32 amino acid peptide hormone synthesized by the parafollicular cells of the thyroid. It causes a rapid, but short lived, reduction in serum calcium and phosphate by promoting the incorporation of those ions in the bones. This effect is opposite to that of parathyroid hormone. Staining for calcitonin may be used for the identification of a spectrum of C cell proliferative abnormalities ranging from C cell hyperplasia to invasive tumors. Staining for calcitonin in medullary carcinoma of the thyroid produces a fine granular pattern in the cytoplasm. Amyloid deposits within the tumor may also exhibit varying degrees of calcitonin activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CALCA